Effect of glutathione depletion on caspase-3 independent apoptosis pathway induced by curcumin in Jurkat cells

Piwocka, Katarzyna; Jaruga, Ewa; Skierski, Janusz; Grądzka, I; Sikora, Ewa

doi:10.1016/s0891-5849(01)00629-3

Cited by 77 publications

(35 citation statements)

References 45 publications

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“…This might explain the decrease in the fragmentation percentage in the curcumin positive control group. This scavenging activity of curcumin lead to attenuated DNA fragmentation due to the elevation of glutathione (Piwocka et al, 2001).…”

Section: Dna Fragmentation Assaymentioning

confidence: 96%

Protective Effect of Curcumin and Chlorophyllin against DNA Mutation Induced by Cyclophosphamide or Benzo[a]pyrene

Ibrahim

El-Behairy

Ghoneim

et al. 2007

Zeitschrift Für Naturforschung C

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The current study was carried out to evaluate the potency of curcumin and chlorophyllin as natural antioxidants to reduce the oxidative stress markers induced by cyclophosphamide (CP) and benzo [a]pyrene [B(a)P] which were used as free radical inducers.For this purpose, 126 male albino rats were used. The animals were assigned into 4 main groups: negative control group; oxidant-treated group (subdivided into two subgroups: cyclophosphamide-treated group and benzo[a]pyrene-treated group); curcumin-treated group; and chlorophyllin-treated group. Liver samples were collected after two days post the oxidant inoculation and at the end of the experimental period (10 weeks). These samples were examined for determination of liver microsomal malondialdehyde (MDA), DNA fragmentation, restriction fragment length polymorphism (RFLP) and 8-hydroxy deoxyguanosine (8-OHdG) concentration.Both CP and B(a)P caused increments in DNA fragmentation percentages, liver microsomal MDA, concentration of 8-OHdG and induced point mutation. Treatment of rats with either curcumin or chlorophyllin revealed lower DNA fragmentation percentages, liver microsomal MDA concentration, concentration of 8-OHdG and prevented induction of mutations, i. e., reversed the oxidative stress induced by CP and B(a)P and proved that they were capable of protecting rats against the oxidative damage evoked by these oxidants.

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Section: Dna Fragmentation Assaymentioning

confidence: 96%

Protective Effect of Curcumin and Chlorophyllin against DNA Mutation Induced by Cyclophosphamide or Benzo[a]pyrene

Ibrahim

El-Behairy

Ghoneim

et al. 2007

Zeitschrift Für Naturforschung C

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“…Curcumin is also a potent inhibitor of protein kinase C, EGF-receptor tyrosine kinase and I kappa B kinase . In addition, curcumin inhibits the expression of c-fos, c-myc and c-jun (Kakar & Roy 1994;Limtrakul et al 2001) and induces apoptosis (Piwocka et al 2001). Despite the fact that 1) curcumin and its derivatives have shown a significant protection against tumourigenesis in several animal tumour bioassay systems (Sharma 1976;Satoskar et al 1986;Huang et al 1992;Goel et al 2001;Mori et al 2001Surh et al 2001 2) depletion in the activities of antioxidant and phase II enzymes occur during tumourigenesis (Perchellet & Perchellet 1989;Sun 1990) 3) cancer chemopreventive agents enhances the activity of these enzymes in target tissues (Wattenberg & Loccia 1990).…”

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confidence: 99%

Dietary Supplementation of Curcumin Enhances Antioxidant and Phase II Metabolizing Enzymes in ddY Male Mice: Possible Role in Protection against Chemical Carcinogenesis and Toxicity

Iqbal

Sharma

Okazaki

et al. 2003

Pharmacology & Toxicology

272

147

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Dietary antioxidants protect laboratory animals against the induction of tumours by a variety of chemical carcinogens. Among possible mechanism of protection against chemical carcinogenesis could be mediated via-antioxidantdependent induction of detoxifying enzymes. Curcumin, a yellow pigment from Curcuma longa, is a major component of turmeric and is commonly used as a spice and food colouring material and exhibits antiinflammatory, antitumour, and antioxidant properties. In this study we therefore investigated the effect of dietary supplementation of curcumin on the activities of antioxidant and phase II-metabolizing enzymes involved in detoxification, and production of reactive oxygen species were quantified in ddY male mice. Dietary supplementation of curcumin (2%, w/v) to male ddY mice for 30 days significantly increased the activities of glutathione peroxidase, glutathione reductase, glucose-6-phosphate dehydrogenase and catalase to 189%, 179%, 189%, and 181% in liver and 143%, 134%, 167% and 115% in kidney respectively as compared with corresponding normal diet fed control (PϽ0.05-0.001). Parallel to these changes, curcumin feeding to mice also resulted in a considerable enhancement in the activity of phase II-metabolizing enzymes viz. glutathione S-transferase and quinone reductase to 1.7 and 1.8 times in liver and 1.1 and 1.3 times in kidney respectively as compared with corresponding normal diet fed control (PϽ0.05-0.01). In general, the increase in activities of antioxidant and phase II-metabolizing enzymes was more pronounced in liver as compared to kidney. The induction of such detoxifying enzymes by curcumin suggest the potential value of this compound as protective agent against chemical carcinogenesis and other forms of electrophilic toxicity. The significance of these results can be implicated in relation to cancer chemopreventive effects of curcumin against the induction of tumours in various target organs.

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“…The MAPK signaling pathways modulate gene expression, mitosis, proliferation, motility, metabolism, and programmed cell death [24]. Furthermore, curcumin is reported to inhibit proliferation and induce apoptosis in T lymphocytes and Jurkat cells [25,26]. Since in the present study, T 3 administration had shown a 37% augmentation in mitochondrial LPX level indicating ROS predominance over antioxidants (Table 1), it is hypothesized that the increased ROS level as a consequence of T 3 -induced hyper-thyroidism may be responsible for the increased cell proliferation probably via MAPK and/or redox active kinases signaling pathways.…”

Section: Resultsmentioning

confidence: 99%

Effect of Turmeric and its Active Principle Curcumin on T3-Induced Oxidative Stress and Hyperplasia in Rat Kidney: A Comparison

et al. 2010

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The present study was designed to compare the potential of turmeric and its active principle curcumin on T 3 -induced oxidative stress and hyperplasia. Adult male Wistar strain rats were rendered hyperthyroid by T 3 treatment (10 lg Á 100 g -1 Á day -1 intraperitoneal for 15 days in 0.1 mM NaOH) to induce renal hyperplasia. Another two groups were treated similarly with T 3 along with either turmeric or curcumin (30 mg kg -1 body weight dayorally for 15 days). The results indicate that T 3 induces both hypertrophy and hyperplasia in rat kidney as evidenced by increase in cell number per unit area, increased protein content, tubular dilation and interstitial edema. These changes were accompanied by increased mitochondrial lipid peroxidation and superoxide dismutase activity without any change in catalase activity and glutathione content suggesting an oxidative predominance. Both turmeric and curcumin were able to restore the level of mitochondrial lipid peroxidation and superoxide dismutase activity in the present dose schedule. T 3 -induced histopathological changes were restored with turmeric treatment whereas curcumin administration caused hypoplasia. This may be due to lower concentration of curcumin in the whole turmeric. Thus it is hypothesized that regulation of cell cycle in rat kidney by T 3 is via reactive oxygen species and curcumin reveres the changes by scavenging them. Although the response trends are comparable for both turmeric and curcumin, the magnitude of alteration is more in the later. Turmeric in the current dose schedule is a safer bet than curcumin in normalizing the T 3 -induced hyperplasia may be due to the lower concentration of the active principle in the whole spice.

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Effect of glutathione depletion on caspase-3 independent apoptosis pathway induced by curcumin in Jurkat cells

Cited by 77 publications

References 45 publications

Protective Effect of Curcumin and Chlorophyllin against DNA Mutation Induced by Cyclophosphamide or Benzo[a]pyrene

Protective Effect of Curcumin and Chlorophyllin against DNA Mutation Induced by Cyclophosphamide or Benzo[a]pyrene

Dietary Supplementation of Curcumin Enhances Antioxidant and Phase II Metabolizing Enzymes in ddY Male Mice: Possible Role in Protection against Chemical Carcinogenesis and Toxicity

Effect of Turmeric and its Active Principle Curcumin on T3-Induced Oxidative Stress and Hyperplasia in Rat Kidney: A Comparison

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