Effect of glutathione on peroxyoxalate chemiluminescence of hypericin as the fluorophore

Silver nanoparticles enhanced a novel TCPO–H 2 O 2 –safranin O chemiluminescence system for determination of 6-mercaptopurine

“…Evaluation of the TCPO concentration at 1×10 -6 to 2×10 -3 molL −1 showed an increase in CL intensity as the TCPO concentration increased to 2×10 -4 molL −1 (Fig.5(a)). This result is consistent with TCPO being the limiting reagent under this reaction condition [7,8,14]. At larger concentration, the CL signal decreased gradually as a result of the self-quenching of TCPO.…”

“…The CL spectra produced from these reactions could be used for different analytical applications, based on the determination of fluorophores, peroxides, catalysts or quenchers used in the PO-CL system. Such multi-scope character of the PO-CL systems enables them to be employed as powerful analytical tools for the detection of a wide variety of analytes such as fluorescent compounds [10], hydrogen peroxide, catecholamines, carboxylic acids, carbonyl [11], DNA [12], protein [13] and amino acids [14].…”

All Eyes on Visible‐Light Peroxyoxalate Chemiluminescence Read‐Out Systems

“…Its versatility has made the PO-CL reaction ap opulara nalytical tool, which has been extensivelye mployed in combination with HPLC to detect a range of analytes (Table 1). PO-CL/HPLC has been employedt o detectt races of H 2 O 2 ,c ompounds that can innately function as fluorophores (e.g.,h ypericin), [30] compounds that can be derivatisedi nto fluorophores (e.g.,a ldehydes that are derivatised with 4-(N,N-dimethylaminosulfonyl)-7-hydrazino-2,1,3-benzoxadiazole to form fluorophores), [29b] ando ther materials that can act as quenchers (e.g.,g lutathione) [30] or enhancers (e.g.,4 -nitrophenol) [29a] for the PO-CL reaction. [31] Compared to fluorescenced etection, CL detection does not requirep hotoexcitation.…”

“…Recently, Kazemi et al. studied the use of hypericin as a novel red fluorophore in PO‐CL and the effect of the reductive amino acid glutathione (GSH) on the overall reaction . GSH is one of the most abundant intracellular small molecules and has a thiol group that makes it a potent reducing agent.…”

“…studied the use of hypericin as an ovel red fluorophore in PO-CL and the effect of the reductivea mino acid glutathione (GSH) on the overall reaction. [30] GSH is one of the most abundant intracellular small molecules and has at hiol group that makesi tapotent reducing agent. Hypericin is famousf or its many roles includingi ts usage against HIV, cancer and as an antidepressant.…”