Effect of glycerin supplementation on the expression of antioxidant and mitochondrial genes in broilers

Abstract

Crude glycerin has been used as an alternative energy source in animal feeding and can thus change both the animal’s performance and the levels of gene expression related to energy production process and the antioxidant system, since the effect of glycerin depends on its degree of purity in the diet. The aim of this study was to evaluate the effect of crude glycerin inclusion (0, 6 and 12%) in the diet has on performance characteristics and mRNA abundance of genes associated with the mitochondrial function (uncoupling protein (UCP), cytochrome C oxidase subunit III (COX III) and adenine nucleotide transporter (ANT)) and those that combat the reactive oxygen species (superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx)) in the liver and muscle of broilers at 42 days of age. Total RNA was extracted from the tissues, and cDNA was amplified using specific primers for the genes under study through real-time reverse transcription polymerase chain reaction; the endogenous gene used was β-actin. There was no difference between glycerin concentrations for weight gain, feed intake or feed conversion. Greater expression of UCP at muscle and higher GPx expression at liver were observed in broilers fed 12% glycerin. In conclusion, the addition of 12% glycerin to broiler diets increased the expression of UCP and GPx, but did not change broiler performance. Therefore, glycerin can be used as an alternative ingredient without losses.

Maternal oxidized soybean oil exposure in rats during lactation damages offspring kidneys via Nrf2/HO‐1 and NF‐κB signaling pathway

“…28 In this study, the activity of GSH-Px and SOD enzymes was increased in the OSO-treated lactating rats. This result is similar to that reported by Araújo et al, 29 who found that both GSH-Px and SOD could promote the decomposition of H 2 O 2 . Overall, we speculated that the increased of GSH-Px and SOD is likely to be an adaptive response to oxidative stress.…”