Effect of grape seed extract on lipid peroxidation, antioxidant activity and peripheral blood lymphocytes in rats exposed to x‐radiation

Abstract

The present studies were designed to evaluate supplemental grape seed extract (GSE) and vitamin E supplements on lipid peroxidation, on antioxidant systems and peripheral blood lymphocytes in rats exposed to x-rays. Three groups of rats were investigated: a control group (CG) received intraperitoneal (i.p.) physiological serum 1 mL/day (n=10), i.p.; a vitamin E group (VG) received 50 mg/kg/day (n=10); an i.p. grape seed extract group received 50 mg/kg/day (n=10). Four weeks later, a 6 Gy radiation dose was given to the rats. Blood samples were taken 24 h later after irradiation and lymphocyte, malondialdehyde (MDA), reduced glutathione (GSH), nitrate, nitrite, reduced ascorbic acid, retinol, beta-carotene and ceruloplasmin concentrations were analysed. The levels of GSH (p<0.05), retinol (p<0.001), beta-carotene (p<0.05) and ceruloplasmin concentration (p<0.001) in the GSE group were found to be higher than in the control group but the level of MDA (p<0.001) and nitrite concentration (p<0.05) in rats supplemented with GSE were found to be lower than in the control group. The results indicate that GSE enhanced the antioxidant status and decreased the incidence of free radical-induced lipid peroxidation in blood samples of rats exposed to x-radiation. The antioxidant effect of GSE given to animals was more effective than vitamin E administered before whole-body irradiation in rats.

Effects of grape seed extract and its ethylacetate/ethanol fraction on blood glucose levels in a model of type 2 diabetes

“…Grapes are widely consumed in many countries and contain many flavonoids, polyphenols and proanthocyanidins with antioxidant properties (Agartan et al, 2004;Bagchi et al, 2000;Enginar et al, 2007;Yamakoshi et al, 2004). Moreover, it has been reported that resveratrol, one of the major antioxidant constituents in grape, shows prevention against diarrhea, heart disease and diabetes (Huang et al, 2001;Vitali et al, 2004;Wang et al, 2002).…”

Evaluation of Antioxidant Parameters in Broiler Chicken after Vaccination and Experimental Challenge with Newcastle Disease Virus

“…The initial parameter to use as an indicator of oxidative stress for the damage of free radicals is the lipid peroxidation mainly affected the phospholipids of cell membrane, therefore, MDA is employed to measure the indicator of lipid peroxidation caused by free radical damage (Enginar et al, 2006). In this study there were not specific differences in the serum MDA levels between four groups but totally the higher blood levels of MDA were observed respectively in the 1, 2, 3 groups.…”

Soapwort extract supplementation alters antioxidant status of serum, liver and heart tissues in growing Japanese quails reared under chronic intermittent cold stress

“…Another study conducted on alloxan-induced diabetic rats by Alli Smith and Adanlawo (2014) reported that saponin extract treatment from the root of Garcinia kola significantly decreased the MDA concentration in the liver, kidney and heart tissues of rats exposed to alloxan. Also, saponin contained in the Yucca schidigera extract was found to alleviate the lipid peroxidation due to irradiation in rabbits exposed to gamma radiation for a period of 4 weeks (Enginar et al 2006). Fidan and Dundar (2008) conducted a study to determine the effects of diet supplementation with saponin-containing Yucca schidigera (Sarsaponin 30, Desert King International, San Diego, CA, USA), Quillaja saponaria (Nutrafito, Desert King International, San Diego, CA, USA), and their mixture powder (Nutrafito plus, Desert King International, San Diego, CA, USA) on deoxyribonucleic acid damage, protein oxidation, and lipid peroxidation in experimentally streptozotocin-induced diabetic rats.…”