Effect of Growth hormones for direct organogenesis in Bael (Aegle marmelos (L.) corr)

Abstract

Aegle marmelos (L.) corr. commonly known as 'Bael' tree is included in the red-listed medicinal tree in South India. Riped and unripe fruits, root, bark and leaves of the tree are used in ayurvedic preparation for various ailments. This study was conducted to standardize the media for multiple shoot induction from different explants under in vitro condition. Among the various explants viz., meristematic tip, nodal bud, tender leaf, auxillary bud and cotyledon tried in different media, auxiliary bud had shown good response for shoot induction in 36 to 39 days. Among the various treatments viz., WPM supplemented with Benzyl Amino Purine (BAP) (0.5 to 3.0mg/lit), Kinetin (KN) (0.5 to 3mg/lit) and Indole acetic acid (IAA) (0.5 to 2mg/lit) for multiple shoot induction, the treatmental combination of WPM supplemented with BAP, KN and IAA, @ of each 0.5 mg/lit resulted in 7.75 shoots per cotyledon. The same combination, ½ strength of the medium with 1mg/lit BAP+2mg/lit NAA+ 1% AC resulted in root formation. Hence, as a means of in vitro conservation using cotyledon as explants microproagation is possible in bael.

Regeneración de yemas adventicias en segmentos de hojas y entrenudos de Balfourodendron riedelianum (Engl.) Engl.

“…Si bien la organogénesis adventicia fue obtenida exitosamente en numerosas especies leñosas como Phellodendron amurense, Aegle marmelos y Murraya koenigii (Khatik y Joshi, 2017;Kumari et al, 2015;Wang et al, 2011), muchas especies arbóreas pueden ser recalcitrantes, pues no es posible su regeneración in vitro. La utilización de explantes se limita, entonces, a embriones zigóticos o partes de plántulas, y estos, a su vez, pueden presentar dificultades -en el cultivo in vitro-por la lixiviación de fenoles (Bonga et al, 2010;Villalobos y Thorpe, 1991).…”