Effect of GSK-3 inhibitor on the proliferation of multipotent male germ line stem cells (mGSCs) derived from goat testis

Zhu, Haijing; Liu, C.; Sun, Junwei; Li, M.; Hua, Jinlian

doi:10.1016/j.theriogenology.2012.01.019

Cited by 30 publications

(35 citation statements)

References 42 publications

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“…Detailed qRT-PCR and gel electrophoresis protocols can be found in previous studies54. The expression levels of genes were analyzed by a quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) as described previously62.…”

Section: Methodsmentioning

confidence: 99%

The Modification of Tet1 in Male Germline Stem Cells and Interact with PCNA, HDAC1 to promote their Self-renewal and Proliferation

Zheng

Zhai

et al. 2016

Sci Rep

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Epigenetic modification plays key roles in spermatogenesis, especially DNA methylation dynamic is important in sustaining normal spermatogenesis. Ten-eleven translocation 1 (Tet1) is not only a key demethylase, which works in specific gene regions, but also crosstalks with partners to regulate epigenetic progress as protein complexes. Dairy goat is an important livestock in China, while the unstable culture system in vitro inhibits optimization of new dairy goat species. The study of epigenetic modification in male germline stem cells (mGSCs) is beneficial to the optimization of adult stem cell culture system in vitro, and the improvement of sperm quality and breeding of selected livestock. In our study, we not only analyzed the morphology, gene expression, DNA methylation and histone methylation dynamic in mouse Tet1 (mTet1) modified mGSCs, we also analyzed the stemness ability by in vivo transplantation and explored the functional mechanism of Tet1 in dairy goat mGSCs. The results showed mTet1 modified mGSCs had better self-renewal and proliferation ability than wild-type mGSCs, mTet1 could also up-regulate JMJD3 to decrease H3K27me3, which also showed to suppress the MEK-ERK pathway. Furthermore, Co-IP analysis demonstrated that TET1 interact with PCNA and HDAC1 by forming protein complexes to comprehensively regulate dairy goat mGSCs and spermatogenesis.

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Section: Methodsmentioning

confidence: 99%

The Modification of Tet1 in Male Germline Stem Cells and Interact with PCNA, HDAC1 to promote their Self-renewal and Proliferation

Zheng

Zhai

et al. 2016

Sci Rep

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“…Dairy goat is one kind of domestic animals of great importance not only for its easily absorbed milk and wonderful meat, but also for its shorter gestation length, earlier sexual maturation, and smaller size than cattle for studying lactation, ruminant nutrition, and fetal development. Male GSCs derived from dairy goat testis are valuable resource for the genetic material conservation and optimization [Hua et al, 2011; Zhu et al, 2012]. Here, we investigated to explore the expression pattern of Boule and study its effects on the meiosis of dairy goat mGSCs.…”

mentioning

confidence: 99%

Expression pattern of Boule in dairy goat testis and its function in promoting the meiosis in male germline stem cells (mGSCs)

Liu

Zhu

et al. 2012

J of Cellular Biochemistry

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Boule is a conserved gene in meiosis, which encodes RNA binding protein required for spermatocyte meiosis. Deletion of Boule was found to block meiosis in spermatogenesis, which contributes to infertility. Up to date, the expression and function of Boule in the goat testis are not known. The objectives of this study were to investigate the expression pattern of Boule in dairy goat testis and their function in male germline stem cells (mGSCs). The results first revealed that the expression level of Boule in adult testes was significantly higher than younger and immature goats, and azoospermia and male intersex testis. Over-expression of Boule promoted the expression of meiosis-related genes in dairy goat mGSCs. The expression of Stra8 was up-regulated by over-expression of Boule analyzed by Western blotting and Luciferase reporter assay. While, Cdc25a, the downstream regulator of Boule, was found not to affect the expression of Stra8, and our data illustrated that Cdc25a did not regulate meiosis via Stra8. The expression of Stra8 and Boule was up-regulated by RA induction. Taken together, results suggest the Boule plays an important role in dairy goat spermatogenesis and that over-expression of Boule may promote spermatogenesis and meiosis in dairy goat.

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“…Nuclei of cells were stained using 4 0 ,6-diamidino-2-phenylindole. Images were captured using a fluorescence microscope (Leica, Hicksville, NY, USA) [16].…”

Section: Immunofluorescencementioning

confidence: 99%

The Tet1 and histone methylation expression pattern in dairy goat testis

et al. 2015

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Effect of GSK-3 inhibitor on the proliferation of multipotent male germ line stem cells (mGSCs) derived from goat testis

Cited by 30 publications

References 42 publications

The Modification of Tet1 in Male Germline Stem Cells and Interact with PCNA, HDAC1 to promote their Self-renewal and Proliferation

The Modification of Tet1 in Male Germline Stem Cells and Interact with PCNA, HDAC1 to promote their Self-renewal and Proliferation

Expression pattern of Boule in dairy goat testis and its function in promoting the meiosis in male germline stem cells (mGSCs)

The Tet1 and histone methylation expression pattern in dairy goat testis

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