Effect of GSM-900 and -1800 signals on the skin of hairless rats. II: 12-week chronic exposures

Sanchez, Sébastien; Masuda, Hiroshi; Billaudel, B.; Haro, E.; Anane, R.; Lévêque, Philippe; Ruffié, Gilles; Lagroye, I.; Veyret, B.

doi:10.1080/09553000600930087

Cited by 18 publications

(11 citation statements)

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“…In vivo , oxidative stress and fibrosis were induced in rat skin after RFR exposure [60]. Other work by our group did not, however, show any effect on proliferation, epidermis thickness or cell structures in rat skin after a single 2 h exposure to GSM‐900 or GSM‐1800 [60a] or a chronic study up to 12 weeks of exposure [60b].…”

Section: Discussionmentioning

confidence: 98%

Human skin cell stress response to GSM‐900 mobile phone signals

Sanchez

Milochau²,

Ruffié

et al. 2006

The FEBS Journal

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In recent years, possible health hazards due to radiofrequency radiation (RFR) emitted by mobile phones have been investigated. Because several publications have suggested that RFR is stressful, we explored the potential biological effects of Global System for Mobile phone communication at 900 MHz (GSM-900) exposure on cultures of isolated human skin cells and human reconstructed epidermis (hRE) using human keratinocytes. As cell stress markers, we studied Hsc70, Hsp27 and Hsp70 heat shock protein (HSP) expression and epidermis thickness, as well as cell proliferation and apoptosis. Cells were exposed to GSM-900 under optimal culture conditions, for 48 h, using a specific absorption rate (SAR) of 2 W x kg(-1). This SAR level represents the recommended limit for local exposure to a mobile phone. The various biological parameters were analysed immediately after exposure. Apoptosis was not induced in isolated cells and there was no alteration in hRE thickness or proliferation. No change in HSP expression was observed in isolated keratinocytes. By contrast, a slight but significant increase in Hsp70 expression was observed in hREs after 3 and 5 weeks of culture. Moreover, fibroblasts showed a significant decrease in Hsc70, depending on the culture conditions. These results suggest that adaptive cell behaviour in response to RFR exposure, depending on the cell type and culture conditions, is unlikely to have deleterious effects at the skin level.

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Section: Discussionmentioning

confidence: 98%

Human skin cell stress response to GSM‐900 mobile phone signals

Sanchez

Milochau²,

Ruffié

et al. 2006

The FEBS Journal

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“…However, we have previously observed that melanocytes are needed to maintain epidermal homoeostasis respective to UV exposure [14,15] Thus, we decided to look at the effect of EMR on epidermal reconstructs containing keratinocytes and melanocytes grown on dead de-epidermized dermis, a physiological model for skin reconstruction. In parallel, to confirm the results of Ennamany et al [9] who used a more physiological dose of exposure (GSM-900, SAR 2 W kg À1 , 6 h) than Sanchez et al [10][11][12] (GSM-900, SAR 2 W kg À1 , 48 h), we also analysed the impact of EMR on reconstructed epidermis without melanocytes. In this study, we measured the variation in the expression of several keratinocyte and melanocyte markers 2, 6 and 24 h after exposure.…”

Section: Introductionmentioning

confidence: 56%

“…Previous studies of EMR on skin were carried out in animal models or reconstructed skin without melanocytes . Using epidermis reconstructed without melanocytes, Sanchez et al .…”

Section: Discussionmentioning

confidence: 99%

“…Some genes had a biphasic expression with a marked increase at 2 h and a decrease at 18 h (HSP 70) or inversely (ET-2). Sanchez et al [10,11] demonstrated that 48-h GSM-1800 exposure did not induce HSP70, 27 and HSC70 in keratinocytes and fibroblasts. On the contrary, they found that 48-h GSM-900 exposure induced a slight significant increase in Hsp70 expression in reconstructed epidermis after 3 and 5 weeks of culture [12].…”

Section: Introductionmentioning

confidence: 99%

“… who used a more physiological dose of exposure (GSM‐900, SAR 2 W kg −1 , 6 h) than Sanchez et al . (GSM‐900, SAR 2 W kg −1 , 48 h), we also analysed the impact of EMR on reconstructed epidermis without melanocytes. In this study, we measured the variation in the expression of several keratinocyte and melanocyte markers 2, 6 and 24 h after exposure.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Exposure to acute electromagnetic radiation of mobile phone exposure range alters transiently skin homeostasis of a model of pigmented reconstructed epidermis

Simon

Daubos

Pain

et al. 2012

Intern J of Cosmetic Sci

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Exposure to electromagnetic radiations (EMR) produced by mobile phone concerns half the world's population and raises the problem of their impact on human health. In this study, we looked at the effects of mobile phone exposure (GSM basic, 900 MHz, SAR 2 mW g(-1) , 6 h) on a model of pigmented skin. We have analysed the expression and localization of various markers of keratinocyte and melanocyte differentiation 2, 6, 18 and 24 h after EMR exposure of reconstructed epidermis containing either only keratinocytes or a combination of keratinocytes and melanocytes grown on dead de-epidermized dermis, using histology, immunohistochemistry and Western blot. No changes were found in epidermal architecture, localization of epidermal markers, presence of apoptotic cells and the induction of p53 in both types of epidermis (with or without melanocytes) after exposure to EMR. In pigmented reconstructs, no change in the location and dendricity of melanocytes and in melanin transfer to neighbouring keratinocytes was detected after EMR exposure. Loricrin, cytokeratin 14 were significantly decreased at 6 h. The level of all markers increased at 24 h as compared to 6 h post-EMR exposure, associated with a significant decrease of the 20S proteasome activity. Our data indicate that exposure to 900 MHz frequency induces a transient alteration of epidermal homoeostasis, which may alter the protective capacity of the skin against external factors. Presence or absence of melanocytes did not modify the behaviour of reconstructs after EMR exposure.

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Cerebral radiofrequency exposures during adolescence: Impact on astrocytes and brain functions in healthy and pathologic rat models

Petitdant

Lecomte

Robidel

et al. 2016

Bioelectromagnetics

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The widespread use of mobile phones by adolescents raises concerns about possible health effects of radiofrequency electromagnetic fields (RF EMF 900 MHz) on the immature brain. Neuro-development is a period of particular sensitivity to repeated environmental challenges such as pro-inflammatory insults. Here, we used rats to assess whether astrocyte reactivity, perception, and emotionality were affected by RF EMF exposures during adolescence. We also investigated if adolescent brains were more sensitive to RF EMF exposures after neurodevelopmental inflammation. To do so, we either performed 80 μg/kg intra-peritoneal injections of lipopolysaccharides during gestation or 1.25 μg/h intra-cerebro-ventricular infusions during adolescence. From postnatal day (P)32 to 62, rats were subjected to 45 min RF EMF exposures to the brain (specific absorption rates: 0, 1.5, or 6 W/kg, 5 days/week). From P56, they were tested for perception of novelty, anxiety-like behaviors, and emotional memory. To assess astrocytic reactivity, Glial Fibrillary Acidic Protein was measured at P64. Our results did not show any neurobiological impairment in healthy and vulnerable RF EMF-exposed rats compared to their sham-exposed controls. These data did not support the hypothesis of a specific cerebral sensitivity to RF EMF of adolescents, even after a neurodevelopmental inflammation. Bioelectromagnetics. 37:338-350, 2016. © 2016 Wiley Periodicals, Inc.

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Effect of GSM-900 and -1800 signals on the skin of hairless rats. II: 12-week chronic exposures

Abstract: The results of this 12-week chronic study do not demonstrate major histological variations in the skin of hairless rats exposed to RFR used in mobile telephony (GSM-900 or -1800).

Cited by 18 publications

References 1 publication

Human skin cell stress response to GSM‐900 mobile phone signals

Human skin cell stress response to GSM‐900 mobile phone signals

Exposure to acute electromagnetic radiation of mobile phone exposure range alters transiently skin homeostasis of a model of pigmented reconstructed epidermis

Cerebral radiofrequency exposures during adolescence: Impact on astrocytes and brain functions in healthy and pathologic rat models

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