Effect of GSM 900-MHz Mobile Phone Radiation on the Reproductive Capacity of<i>Drosophila melanogaster</i>

Panagopoulos, Dimitris J.; Karabarbounis, A.; Margaritis, Lukas H.

doi:10.1081/jbc-120039350

Cited by 84 publications

(74 citation statements)

References 44 publications

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“…Showing an increase in fertility [15] is in some contradiction to the data presented in [9][10][11][12][13][14]. In our opinion, the experimental conditions in this work [15] were different from those in [9][10][11][12][13][14]. Specifically, it is feasible that the microwave-exposed larvae of Drosophila were exposed to microwaves in cultural medium with high water content that led to intensive absorption of microwave energy by the media.…”

Section: Introductioncontrasting

confidence: 49%

“…Exposure to microwaves (1.900 MHz; SAR -1.4 W/kg) of Drosophila melanogaster, during the 10-day developmental period (60 min at 11 AM and 60 min at 4 PM daily) from egg laying through pupation increased numbers of offspring, elevated hsp70 levels, increased serum response element (SRE) DNA-binding and induced the phosphorylation of the nuclear transcription factor, ELK-1 as demonstrated Weisbrot et al [15]. Showing an increase in fertility [15] is in some contradiction to the data presented in [9][10][11][12][13][14]. In our opinion, the experimental conditions in this work [15] were different from those in [9][10][11][12][13][14].…”

Section: Introductionmentioning

confidence: 41%

“…The same 10 GHz EMF exposure (with SAR approximately 9.8 mW/kg) decreases fertility of Drosophila melanogaster causing the decrease in the egg production [10]. Exposure of the flies to the carrier frequency 900 MHz "modulated" by human voice, (speaking emission), for 6 min per day during the first 2-5 days of their adult lives, decreased the reproductive capacity of Drosophila melanogaster by 50-60%, whereas the corresponding "non-modulated" field decreased the reproductive capacity by 15-20% as shown by Panagopoulos et al [12]. In our opinion, the described "modulation" effect may be caused by an energy saving process in cellular phone in silence mode.…”

Section: Introductionmentioning

confidence: 87%

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Effects of microwaves on the puffing pattern of D. melanogaster

et al. 2011

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The influence of electromagnetic field exposure on puffing pattern of salivary gland polythene chromosomes, viability and fertility of Drosophila melanogaster of the wild type Canton-S line was studied. Experimental conditions: Electromagnetic field characteristics: frequency — 36.64 GHz, power density — 0.4 W/m2, exposure time −10 seconds. Electromagnetic field exposure was conducted on the egg stage. Results: in larvae developed from the exposed eggs 3 of 8 chromosomal puffs tested (71CE, 82EF, and 83E) had significantly smaller dimensions than these in control at the prepupal stage. Viability of Drosophila estimated by the number of adult flies hatched from exposed eggs decreased, while the number of dominant lethal mutations increased. Conclusion: the exposure to a low-level microwave irradiation suppressed puffing activity at ecdysone-inducible loci of Drosophila polythene chromosomes, increased frequency of dominant lethal mutations and decreased Drosophila viability but did not influence Drosophila fertility.

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Section: Introductioncontrasting

confidence: 49%

Section: Introductionmentioning

confidence: 41%

Section: Introductionmentioning

confidence: 87%

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Effects of microwaves on the puffing pattern of D. melanogaster

et al. 2011

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“…1,[12][13][14] Oviposition is the process of egg-laying in D. melanogaster that is dependent on responses of the fly to environmental stimuli (such as chemicals, physical conditions of the substrate, temperature, light, population density, and humidity). [15][16][17][18][19] D. melanogaster possesses sensory neurons (such as taste 20,21 and touch 22,23 sensing systems) to detect various environmental cues prior to selecting preferred sites for oviposition and egg laying. [15][16][17][18][19] Therefore, oviposition is used as a metric quantity to provide a clear indication of flies' sensory system health and their overall fitness (the ability to survive and reproduce).…”

mentioning

confidence: 99%

“…[15][16][17][18][19] D. melanogaster possesses sensory neurons (such as taste 20,21 and touch 22,23 sensing systems) to detect various environmental cues prior to selecting preferred sites for oviposition and egg laying. [15][16][17][18][19] Therefore, oviposition is used as a metric quantity to provide a clear indication of flies' sensory system health and their overall fitness (the ability to survive and reproduce). 24,25 It is used predominately in developmental, 26 chemical screening, 27 and toxicology 28,29 studies as a simple and effective readout indicator.…”

mentioning

confidence: 99%

Agar-polydimethylsiloxane devices for quantitative investigation of oviposition behaviour of adult Drosophila melanogaster

et al. 2015

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Drosophila melanogaster (fruit fly) is a model organism and its behaviours including oviposition (egg-laying) on agar substrates have been widely used for assessment of a variety of biological processes in flies. Physical and chemical properties of the substrate are the dominant factors affecting Drosophila's oviposition, but they have not been investigated precisely and parametrically with the existing manual approaches. As a result, many behavioral questions about Drosophila oviposition, such as the combined effects of the aforementioned substrate properties (e.g., exposure area, sugar content, and stiffness) on oviposition and viability, and their threshold values, are yet to be answered. In this paper, we have devised a simple, easily implementable, and novel methodology that allows for modification of physical and chemical composition of agar substrates in order to quantitatively study survival and oviposition of adult fruit flies in an accurate and repeatable manner. Agar substrates have been modified by surface patterning using single and hexagonally arrayed through-hole polydimethylsiloxane (PDMS) membranes with various diameters and interspacing, as well as by substrate stiffness and sugar content modification via alteration of chemical components. While pure PDMS substrates showed a significant lethal effect on flies, a 0.5 mm diameter through-hole access to agar was found to abruptly increase the survival of adult flies to more than 93%. Flies avoided ovipositing on pure PDMS and on top of substrates with 0.5 mm diameter agar exposure areas. At a hole diameter of 2 mm (i.e., 0.25% exposure area) or larger, eggs were observed to be laid predominately inside the through-holes and along the edges of the PDMS-agar interface, showing a trending increase in site selection with 4 mm (i.e., 1% exposure area threshold) demonstrating natural oviposition rates similar to pure agar. The surfacemodified agar-PDMS hybrid devices and the threshold values reported for the substrate physical and chemical conditions affecting oviposition are novel; therefore, we advocate their use for future in-depth studies of oviposition behaviour in Drosophila melanogaster with accuracy and repeatability. The technique is also useful for development of novel assays for learning and decision-making studies as well as miniaturized devices for self-assembly of eggs and embryonic developmental investigations. V C 2015 AIP Publishing LLC. [http://dx

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Exposure of rat brain to 915 MHz GSM microwaves induces changes in gene expression but not double stranded DNA breaks or effects on chromatin conformation

Belyaev

Koch

Terenius

et al. 2006

Bioelectromagnetics

112

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We investigated whether exposure of rat brain to microwaves (MWs) of global system for mobile communication (GSM) induces DNA breaks, changes in chromatin conformation and in gene expression. An exposure installation was used based on a test mobile phone employing a GSM signal at 915 MHz, all standard modulations included, output power level in pulses 2 W, specific absorption rate (SAR) 0.4 mW/g. Rats were exposed or sham exposed to MWs during 2 h. After exposure, cell suspensions were prepared from brain samples, as well as from spleen and thymus. For analysis of gene expression patterns, total RNA was extracted from cerebellum. Changes in chromatin conformation, which are indicative of stress response and genotoxic effects, were measured by the method of anomalous viscosity time dependencies (AVTD). DNA double strand breaks (DSBs) were analyzed by pulsed-field gel electrophoresis (PFGE). Effects of MW exposure were observed on neither conformation of chromatin nor DNA DSBs. Gene expression profiles were obtained by Affymetrix U34 GeneChips representing 8800 rat genes and analyzed with the Affymetrix Microarray Suite (MAS) 5.0 software. In cerebellum from all exposed animals, 11 genes were upregulated in a range of 1.34-2.74 fold and one gene was downregulated 0.48-fold (P < .0025). The induced genes encode proteins with diverse functions including neurotransmitter regulation, blood-brain barrier (BBB), and melatonin production. The data shows that GSM MWs at 915 MHz did not induce PFGE-detectable DNA double stranded breaks or changes in chromatin conformation, but affected expression of genes in rat brain cells.

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Effect of GSM 900-MHz Mobile Phone Radiation on the Reproductive Capacity ofDrosophila melanogaster

Cited by 84 publications

References 44 publications

Effects of microwaves on the puffing pattern of D. melanogaster

Effects of microwaves on the puffing pattern of D. melanogaster

Agar-polydimethylsiloxane devices for quantitative investigation of oviposition behaviour of adult Drosophila melanogaster

Exposure of rat brain to 915 MHz GSM microwaves induces changes in gene expression but not double stranded DNA breaks or effects on chromatin conformation

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