Effect of guinea‐pig purified immunoglobulin G1 on the responsiveness of tracheal, aortic, vas deferens and ileum smooth muscles

Abstract

The present results show that the in vitro application of purified IgG1 can produce non-specific smooth muscle hyperreactivity and hypersensitivity. So, IgG1 can be considered as the main factor involved in the genesis of sensitization-induced hyperresponsiveness, and probably play a great role in hyperreactivity observed during allergic diseases and anaphylaxis.

Changes in resting membrane potential induced by active sensitization in the guinea-pig vas deferens

“…However, interestingly, denervation-induced supersensitivity in the guineapig vas deferens also leads to partial depolarization of the resting membrane potential (E r ) in relation with a decreased density of functional Na + -K + pump sites (Fleming and Westfall 1975;Gerthoffer et al 1979;Wong et al 1981). Thus, in the present study, by analogy with our previous findings of hyperreactivity in actively sensitized guinea-pig vas deferens (Bidon et al 1994(Bidon et al , 1995, we have investigated whether hyperresponsiveness, induced by active sensitization with ovalbumin of the guinea-pig vas deferens, was associated with depolarization and (or) hyperpolarization of E r . As we did find an alteration in E r after active sensitization, the possible involvement of the Na + -K + pump was analyzed in another series of experiments by quantifying the total concentration of [ 3 H]ouabain binding sites and by measuring 86 Rb efflux and Na + -K + contents using vas deferens from control and sensitized guinea-pigs.…”

“…Isolated tissues were prepared as previously described (Bidon et al 1994(Bidon et al , 1995. Briefly, guinea-pigs were killed by cervical dislocation and bled from the carotids.…”

Nonlinear mixed effects models applied to cumulative concentration–response curves

“…In our laboratory, we currently carry out experiments on b-adrenoceptor activation, which mediates a concentrationdependent relaxation in rat, guinea-pig and equine isolated blood vessels. [6][7][8][9] To illustrate the use of nlme models, we chose the data obtained in a study of b-adrenoceptor-mediated effects in rat isolated thoracic aorta rings. [9] The experiments were performed in 12-week-old male Wistar Kyoto (WKY) rats.…”

Loss of nitric oxide release in passively sensitized guinea‐pig aorta with purified immunoglobulin Gl

“…The animals were bled 22-37 days after the first injection, and the serum was stored at -30"C until analysis. IgG| was highly purified by a chromatographic technique using a Protein A-sepharose column, and its amount was measured with the enzyme linked immimoassay {ELISA) [4]. in a second step, a passive in vitro sensitization of aortic rings was performed using a modified technique from Regal [8].…”

“…develop an increased contractile response to various pharmacological stimuli [1-3.5.6|. Active sensitization of animals, especially guinea-pig, to ovalbumen and passive relevant experimental models to study smooth muscle hyperreactivity [3][4][5][6]. In aorta and airways, one hypothesis is that pathophysiological disorders are reliable to a loss of relaxant factors released in normal conditions from the endothelium |3[ or the epithelium [7].…”