Effect of HBT on the stability of laccase during the decolourization of textile wastewaters

Khlifi, Rim; Sayadi, Sami; Belbahri, Lassaâd; Woodward, Stephen; Mechichi, Tahar

doi:10.1002/jctb.2253

Cited by 25 publications

(11 citation statements)

References 34 publications

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“…This was because at very low concentrations the quantity of organic content will be low. Similar results were observed in the work of Khlifi et al (2010) where optimal decolorization occurred with 20% effluent at pH 5.…”

Section: Effect Of Inert Substrate Concentration (Puf)supporting

confidence: 89%

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Biodecolorization and Bioremediation of Denim Industrial Wastewater by Adapted Bacterial Consortium Immobilized on Inert Polyurethane Foam (PUF) Matrix: A First Approach with Biobarrier Model

Rajendran¹,

Prabhavathi²,

Karthiksundaram³

et al. 2015

Pol J Microbiol

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A b s t r a c tThe present experiments were studied on bioremediation of denim industry wastewater by using polyurethane foam (PU foam) immobilized bacterial cells. About 30 indigenous adapted bacterial strains were isolated from denim textile effluent out of which only four isolates were found to be efficient against crude indigo carmine degradation using broth decolorization method. The selected bacterial strains were identified as Actinomyces sp., (PK07), Pseudomonas sp., (PK18), Stenotrophomonas sp., (PK23) and Staphylococcus sp., (PK28) based on microscopic and biochemical characteristics. The bacterial immobilized cells have the highest number of viable cells (PK07, PK18, PK23 and PK28 appeared to be 1 × 10 8 , 1 × 10 9 , 1 × 10 6 and 1 × 10 7 CFU/ml respectively) and maximum attachment efficiency of 92% on PU foam. The complete degradation using a consortium of PU foam immobilized cells was achieved at pH 6, 27°C, 100% of substrate concentration and allowed to develop biofilm for one day (1.5% W/V). In SEM analysis, it was found that immobilization of bacterial cells using PUF stably maintained the production of various extracellular enzymes at levels higher than achieved with suspended forms. Finally, isatin and anthranilic acid were found to be degradation products by NMR and TLC. The decolorized dye was not toxic to monkey kidney cell (HBL 100) at a concentration of 50 µl and 95% of cell viability was retained. A mathematical model that describes bacterial transport with biodegradation involves a set of coupled reaction equations with non-standard numerical approach based on the time step scheme. K e y w o r d s: biobarrier model, bioflim, bioremediation, cytotoxicity test, polyurethane foam, secondary metabolites

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Section: Effect Of Inert Substrate Concentration (Puf)supporting

confidence: 89%

“…Thus maximum degradation was seen in 27°C. In the similar experiments (Khlifi et al, 2010) were conducted at different temperatures, 20°C, 25°C, 30°C, 35°C. The maximum degradation of waste water was found at 30°C.…”

Section: Effect Of Inert Substrate Concentration (Puf)mentioning

confidence: 99%

Biodecolorization and Bioremediation of Denim Industrial Wastewater by Adapted Bacterial Consortium Immobilized on Inert Polyurethane Foam (PUF) Matrix: A First Approach with Biobarrier Model

Rajendran¹,

Prabhavathi²,

Karthiksundaram³

et al. 2015

Pol J Microbiol

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“…A decolorization (%) of 80% was obtained by Khlifi et al using 1‐hydroxybenzotriazole (HBT) as mediator . In fact, the authors obtained complete decolorization of the diluted industrial textile effluent (20%) by Trametes trogii laccase (5 U mL −1 ) mediated with 10 mmol L −1 of HBT at 50 °C and pH 5 within 6 h . However, the use of mediator systems often lead to toxicity since most of the effective mediators such as HBT are very toxic even at low concentrations …”

Section: Resultsmentioning

confidence: 99%

A comparative study of an industrial effluent treatment using enzymatic and alkaline adapted consortium assays

Younes

Ellouze

Sayadi

2012

J of Chemical Tech & Biotech

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BACKGROUND: Dyeing effluents are the main source of water pollution from textile industries. Consequently, the treatment of such effluents is of environmental concern. RESULTS: Decolorization of a textile industry effluent was investigated using, for the first time, the trimeric Scytalidium thermophilum laccase without mediator addition. The maximum effluent decolorization (50%) was achieved after 8 h using a diluted effluent (5 fold) and 4 units of crude enzyme, operating at 50 °C and pH 4. Characterization of raw effluent presents two peaks as demonstrated by the UV‐visible spectrum, showing an intensive peak around 670 nm and a smaller one at 290 nm. At the end of the enzymatic treatment, these two peaks disappeared and a new peak emerged at 280 nm. Because laccase catalyzed effluent decolorization percentage did not exceed 50%, an alkaline adapted consortium treatment assay of this effluent was carried out. An alkaline adapted consortium was used at bench scale in order to decolorize the raw effluent. After 24 h of exposure, color and COD (chemical oxygen demand) removal rates reached 73% and 71%, respectively, at pH 11. CONCLUSION: The adapted consortium treatment of the industrial effluent is economically very interesting since the treatment is costless and can be applied at pilot or industrial scales. © 2012 Society of Chemical Industry

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“…In this sense, ligninolytic enzymes have been involved in the degradation of xenobiotic and recalcitrant aromatic compounds, industrial dyes (Khlifi et al 2009 ; Daâssi et al 2012 ), polycyclic aromatic hydrocarbons (PAHs) (Lee et al 2013 ), pesticides (Xiao et al 2011 ), dioxins (Sato et al 2002 ), chlorophenols (Li et al 2011 ), explosives (Cheong et al 2006 ) and kraft pulp bleaching (Moldes et al 2010 ).…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic and metabolic diversity of Tunisian forest wood-degrading fungi: a wealth of novelties and opportunities for biotechnology

et al. 2016

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In this study, 51 fungal strains were isolated from decaying wood samples collected from forests located in the Northwest of Tunisia in the vicinity of Bousalem, Ain Draham and Kef. Phylogenetic analysis based on the sequences of the internal transcribed spacers of the ribosomal DNA showed a high diversity among the 51 fungal isolates collection. Representatives of 25 genera and 29 species were identified, most of which were members of one of the following phyla (Ascomycota, Basidiomycota and Zygomycota). In addition to the phylogenetic diversity, a high diversity of secreted enzyme profiles was also detected among the fungal isolates. All fungal strains produced at least one of the following enzymes: laccase, cellulase, protease and/or lipase.Electronic supplementary materialThe online version of this article (doi:10.1007/s13205-015-0356-8) contains supplementary material, which is available to authorized users.

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Effect of HBT on the stability of laccase during the decolourization of textile wastewaters

Abstract: BACKGROUND: This study concerns the assessment of laccase stability during textile industry effluent treatment by laccase in the presence of a mediator

Cited by 25 publications

References 34 publications

Biodecolorization and Bioremediation of Denim Industrial Wastewater by Adapted Bacterial Consortium Immobilized on Inert Polyurethane Foam (PUF) Matrix: A First Approach with Biobarrier Model

Biodecolorization and Bioremediation of Denim Industrial Wastewater by Adapted Bacterial Consortium Immobilized on Inert Polyurethane Foam (PUF) Matrix: A First Approach with Biobarrier Model

A comparative study of an industrial effluent treatment using enzymatic and alkaline adapted consortium assays

Phylogenetic and metabolic diversity of Tunisian forest wood-degrading fungi: a wealth of novelties and opportunities for biotechnology

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