Effect of helium/neon laser irradiation on nerve growth factor synthesis and secretion in skeletal muscle cultures

Schwartz, Fidi; Brodie, Chaya; Appel, Elana; Kazimirsky, Gila; Shainberg, Asher

doi:10.1016/s1011-1344(02)00378-0

Cited by 16 publications

(18 citation statements)

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“…Detail analysis of LLLT could also be due to the fact that nerve growth factor synthesis can be regulated by lowlevel irradiation. 45 Nerve growth factor is required for the survival of developing sympathetic and sensory neurons, degeneration are hematogenous phagocytes, 43 with Schwann cells and macrophages also participating in myelin removal to phagocytize the debris left from the breakdown of myelin sheath. Our results showed significant decrease in Wallerian degeneration after LLLT in the experimental group (Figure 2), further approving that laser therapy affects axonal growth and regeneration (Figure 3).…”

Section: Discussionmentioning

confidence: 99%

“…Detail analysis of LLLT could also be due to the fact that nerve growth factor (NGF) synthesis can be regulated by low-level irradiation. 45 Nerve growth factor is required for the survival of developing sympathetic and sensory neurons, especially when the nerve fiber is injured and neurons need to be protected from apoptosis, S36 a biochemical process that results in change of cell morphology and leads to death. Further research will be needed in the future to study such factors.…”

Section: Discussionmentioning

confidence: 99%

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Stimulation Effect of Low Level Laser Therapy on Sciatic Nerve Regeneration in Rat

et al. 2017

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IntroductionThe nerve fiber or axon, is responsible for transmitting information between neuron cells. 1 In humans, axons are generally on the scale of 1 μm thick and some are more than 1 m long, 1 and in the peripheral nerve system (PNS), they are either unmyelinated or myelinated by Schwann cells.2 These cells are mainly wrapped around axons and form a multi-layered sheath preparing myelin structures for axons. 2,3 In case of injury, Schwann cells are activated and play a key role in nerve repair mechanism, not only concerning Wallerian degeneration and remyelination in the injured position, but also in promoting axonal growth. 4 Wallerian degeneration occurs when an axon is cut or injured, in which the axon degenerates distal to the injury site. [5][6][7] This is followed by secondary phenomena such as breakdown of the myelin sheet and activation of the Schwann and macrophages that clear up the debris. 8,9Nerve injuries are divided into 3 basic categories of neuropraxia, axonotmesis and neurotmesis based on the extent of injury, pathology and physiology according to Sir Herbert Seddon's scheme, 5,6 neuropraxia, the mildest nerve injury, is a temporary loss of sensory function. 10The injured site usually fully recovers within few weeks or months, and no Wallerian degeneration occurs in this type of injury. Axonotmesis is when axons are more seriously damaged and interrupted. 10 In this type of injury, the surrounding epineurium and perineurium layers remain almost intact, but there could be only partial recovery depending on the extent of injury to the axons. In addition, Wallerian degeneration may occur distal to Methods:In this research work, the effect of LLLT (780 nm) on the regeneration process and reconstruction of injured peripheral right side sciatic nerve was investigated. Twelve adult male Wistar rats underwent surgery in aseptic conditions under general anesthesia to induce a lesion to their right side sciatic nerve according to standard protocol. Before suturing the location, only the experimental group was treated by laser. The damaged nerve was directly irradiated with (2 J, 100 mW, 40 seconds). The irradiation procedure was terminated in 21 days with little improvement (4 J, 200 mW, 40 seconds) across the skin surface of experimental group. Rats were selected randomly from each group to be sacrificed on different periods and histopathological examination was carried out on the extracted nerves.Results: Significant acceleration of revascularization and angiogenesis of the injury site was observed in the experimental group. Furthermore, a reduction of hemorrhages and increase in blood supply was observed. Also, Wallerian degeneration decreased while higher axonal density compared to the control rats was observed. Moreover, the cross-section analysis of the injured area on the 14th and 21st days as post-surgery showed that the nerve sheath diameter in the lesion area of the experimental group was reduced. While the ratio between thicknesses increased in the control group. Conclusion:The results of...

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Stimulation Effect of Low Level Laser Therapy on Sciatic Nerve Regeneration in Rat

et al. 2017

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“…Our study is consistent with another report that 3T3 fibroblasts irradiated with laser energy at 2.16 J/cm 2 increased cell proliferation, whereas fibroblasts irradiated with higher energy (3.24 J/cm 2 ) did not increase cell proliferation as compared to the control group. 24 However, Schwartz et al 25 reported that helium/neon irradiation on skeletal muscle increased nerve growth factor (NGF) expression at high energy treatment (3 J/cm 2 ). This difference may depend on the laser irradiation model and the stimulation source.…”

Section: Discussionmentioning

confidence: 99%

Low‐level laser irradiation promotes cell proliferation and mRNA expression of type I collagen and decorin in porcine achilles tendon fibroblasts In Vitro

Chen

Tsai

Wang

et al. 2008

Journal Orthopaedic Research

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Achilles tendon problems are commonly encountered in sports medicine and low-level laser therapy (LLLT) is widely used in rehabilitative applications to decrease pain, reduce inflammatory processes, and promote tissue healing. This study examined the effects on the proliferation of porcine Achilles tendon fibroblasts and gene expression, using different doses of low-level laser irradiation (LLLI). Four groups of identically cultured fibroblasts were exposed to LLLI and harvested after 24 h. The control group (Group 1) was subjected to no LLLI. Other groups received 1 J/cm 2 (Group 2), 2 J/cm 2 (Group 3), and 3 J/cm 2 (Group 4), respectively. Cell proliferation and mRNA expressions of type I collagen and decorin were then measured. When compared to the control group, the cell proliferation of irradiated Achilles tendon fibroblasts in the other three groups increased significantly by 13% AE 0.8% (Group 2), 30% AE 0.4% (Group 3), and 12% AE 0.6% (Group 4) respectively. But progressively higher laser intensity did not achieve a correspondingly higher cell proliferation effect in Achilles tendon fibroblasts. The mRNA expressions of decorin and type I collagen in fibroblasts with LLLI were significantly higher (p < 0.05). Therefore, suitable dosages of LLLI may result in more effective tissue healing by promoting type I collagen and decorin synthesis. However, these positive effects of LLLI on the repair of the Achilles tendon in humans should be further investigated in clinic. ß

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“…No presente estudo o laser foi aplicado sobre a pele e portanto sobre o tecido muscular, onde se pode considerar a possibilidade de um aumento dos ní-veis do fator de crescimento do nervo, que é um fator neurotrópico secretado pelos músculos esqueléticos, já que foi demonstrado que o laser de baixa intensidade, no comprimento de onda de 632 nm, possibilita tal resposta 51 .…”

Section: Discussionunclassified

Efeito do laser de baixa intensidade (660 nm) na regeneração do nervo isquiático lesado em ratos

et al. 2010

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Os nervos periféricos são estruturas que, ao sofrerem lesões, podem originar incapacidades motoras e sensitivas importantes. O laser de baixa intensidade é um dos diversos recursos terapêuticos para promover a regeneração nervosa precoce, mas ainda não há consenso sobre sua utilização. O objetivo deste estudo foi investigar, por meio de avaliação funcional, o efeito da terapia a laser de baixa intensidade (660 nm) na regeneração do nervo isquiático após esmagamento. Foram utilizados 18 ratos (Wistar) submetidos à lesão do nervo isquiático divididos em dois grupos, controle e grupo laser, submetido ao tratamento a laser (AsGaAl, 660 nm, 10J/cm2, 30 mW e 0,06 cm²) por 21 dias no local da lesão. Para a avaliação funcional, foi aplicado o índice funcional do ciático (IFC) no pré-operatório e nos 7º, 14º e 21º dias de pós-operatório. Quando comparados o IFC dos grupos no 14o dia de pós-operatório, foi encontrada melhora significante no grupo laser em relação ao controle. Na amostra analisada e nos parâmetros utilizados, pôde-se constatar que a aplicação do laser foi eficaz na recuperação funcional precoce do nervo ciático esmagado.

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Effect of helium/neon laser irradiation on nerve growth factor synthesis and secretion in skeletal muscle cultures

Cited by 16 publications

References 0 publications

Stimulation Effect of Low Level Laser Therapy on Sciatic Nerve Regeneration in Rat

Stimulation Effect of Low Level Laser Therapy on Sciatic Nerve Regeneration in Rat

Low‐level laser irradiation promotes cell proliferation and mRNA expression of type I collagen and decorin in porcine achilles tendon fibroblasts In Vitro

Efeito do laser de baixa intensidade (660 nm) na regeneração do nervo isquiático lesado em ratos

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