Effect of hemp fiber on UVB-induced epidermal cell proliferation and PCNA expression

Throughout life, the human eye is continuously exposed to sunlight and artificial lighting. Ambient light exposure can lead to visual impairment and transient or permanent blindness. To mimic benign light stress conditions, Mus musculus eyes were exposed to low-energy UVB radiation, ensuring no severe morphological changes in the retinal structure post-exposure. We performed RNA-seq analysis to reveal the early transcriptional changes and key molecular pathways involved before the activation of the canonical cell death pathway. RNA-seq analysis identified 537 genes that were differentially modulated, out of which 126 were clearly up regulated (>2-fold, P < .01) and 51 were significantly down regulated (<2-fold, P < .01) in response to UVB irradiation in the mouse retina. Gene ontology analysis revealed that UVB exposure affected pathways for cellular stress and signaling (eg, Creb3, Ddrgk1, Grin1, Map7, Uqcc2, Uqcrb), regulation of chromatin and gene expression (eg, Chd5, Jarid2, Kat6a, Smarcc2, Sumo1, Zfp84), transcription factors (eg, Asxl2, Atf7, Per1, Phox2a, Rxra), RNA processing, and neuronal genes (eg, B4gal2, Drd1, Grm5, Rnf40, Rnps1, Usp39, Wbp4). The differentially expressed genes from the RNA-seq analysis were validated by quantitative PCR. Both analyses yielded similar gene expression patterns. The genes and pathways identified here improve the understanding of early transcriptional responses to UVB irradiation. They may also help in elucidating the genes responsible for the inherent susceptibility of humans to UVB-induced retinal diseases. K E Y W O R D Sphoto-toxicity, retina, transcriptome, RNA-seq, retinal diseases, UVB

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Transcriptome analysis for UVB‐induced phototoxicity in mouse retina

Kim

Nam

et al. 2017

Environmental Toxicology

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Relationship between complications of type 2 diabetes and thyroid nodules

Zhang

Wang

Gong

et al. 2023

Current Medical Research and Opinion

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Photoprotective Effects of Oral L-Glutathione Supplementation on Epidermal Hyperplasia in UVB Irradiated Balb/C Mice

Ghazali¹,

Nagapan²,

Basri³

et al. 2019

Pakistan J. of Nutrition

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Effect of hemp fiber on UVB-induced epidermal cell proliferation and PCNA expression

Cited by 3 publications

References 29 publications

Transcriptome analysis for UVB‐induced phototoxicity in mouse retina

Transcriptome analysis for UVB‐induced phototoxicity in mouse retina

Relationship between complications of type 2 diabetes and thyroid nodules

Photoprotective Effects of Oral L-Glutathione Supplementation on Epidermal Hyperplasia in UVB Irradiated Balb/C Mice

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