Effect of heparin on a haemagglutinin of porcine reproductive and respiratory syndrome virus

Jusa, Enuh Raharjo; Inaba, Yuji; Kong, Miaomiao; Asagoe, Tetsuo; Uwatoko, Kazuhiro; Hirose, Osamu

doi:10.1016/s0034-5288(97)90201-0

Cited by 33 publications

(36 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our results from the present paper indicate that heparin inhibited HA by EAV as well as by ADV [13] and PRRSV [6], but failed to inhibit HA by PIV-3. This finding indicates that heparin acts selectively, inhibiting HA by EAV.…”

Section: Discussionmentioning

confidence: 54%

“…Our findings indicate that heparin may bind to EAV hemagglutinin, presumably occupying the sites necessary for attachment of the hemagglutinin to erythrocytes as well as those of PRRSV [5], herpesviruses [13], and bunyaviruses [6].…”

Section: Discussionmentioning

confidence: 99%

“…ADV and PIV-3 hemaglutinins were prepared from the infected culture fluids of CPK and MDBK cells infected with ADV and PIV-3, respectively, by the method described in the previous papers [3,5].…”

Section: Virusesmentioning

confidence: 99%

“…A heparin-like molecule on the cell surface has also been known to serve as a cellular receptor for herpesviruses [8,14], and PRRSV [5,6]. Recently, we also found that the growth of EAV was inhibited by heparin [1].…”

mentioning

confidence: 98%

“…The cell-medium mixture was We have reported that equine arteritis virus (EAV), a member of the genus Arterivirus [9], agglutinates mouse and chicken erythrocytes [7]. In previous papers, the inhibitory effect of heparin on the growth or hemagglutination of the following viruses has been reported: herpes simplex virus (HSV) [10,12,15,16], bovid herpesvirus 1 (BHV-1) [14] and Aujeszkey's disease virus (ADV) [8,13] of the family Herpesviridae, subfamily Alphaherpesvirinae, genus Simplexvirus or Varicellovirus; bovine ephemeral fever, Kimberley and Berrimah viruses of the family Rhabdoviridae, genus Ephemerovirus [4,11]; Akabane and Aino viruses of the family Bunyaviridae [4,11]; and porcine reproductive and respiratory syndrome virus (PRRSV) [6].…”

mentioning

confidence: 99%

See 4 more Smart Citations

Effect of Heparin on Hemagglutination by Equine Arteritis Virus.

et al. 1998

View full text Add to dashboard Cite

ABSTRACT. Heparin inhibited hemagglutination (HA) by equine arteritis virus (EAV) as well as did HA by Aujeszky's disease virus (ADV), but failed to inhibit HA by parainfluenza virus type 3 (PIV-3). The minimal concentration of heparin required to inhibit 8 HA U of EAV was 0.1 U/ml. In addition, most EAV hemagglutinin was retained by heparin acrylic beads, as was ADV hemagglutinin, but was not PIV-3 hemagglutinin. Mouse erythrocytes failed to combine with the HA inhibitory factor of heparin. However, mouse erythrocytes treated with heparinase had greatly reduced agglutinability by EAV. All these findings suggest that a heparin-like molecule on the surface of mouse erythrocytes serves as the virus-cell receptor. -KEY WORDS: equine arteritis virus, hemagglutinin, heparin, inhibitory effect.

show abstract

Section: Discussionmentioning

confidence: 54%

Section: Discussionmentioning

confidence: 99%

Section: Virusesmentioning

confidence: 99%

mentioning

confidence: 98%

mentioning

confidence: 99%

See 3 more Smart Citations

Effect of Heparin on Hemagglutination by Equine Arteritis Virus.

et al. 1998

View full text Add to dashboard Cite

show abstract

Glycosaminoglycan-Binding Ability Is a Feature of Wild-Type Strains of Herpes Simplex Virus Type 1

et al. 2002

View full text Add to dashboard Cite

Adaptation of some viruses to replication in cultured cells selects variants that due to alterations in the viral attachment proteins convert to using heparan sulfate (HS) as initial receptor. We report that the nucleotide sequence of herpes simplex virus type 1 (HSV-1) glycoprotein C (gC), a principal attachment component of the virus, remained unchanged during adaptation of wild-type strains to cultured cells. Likewise, amino acid residues critical for binding of gC to HS were conserved in viral strains that replicated in vivo in different human tissues. Moreover wild-type HSV-1 strains derived directly from clinical specimens were, similar to their cell culture propagated progeny viruses and common laboratory strains, sensitive to heparin and demonstrated impairment in their ability to infect HS/chondroitin sulfate deficient cells. These results demonstrate that the HS-binding ability is a feature of wild-type strains of HSV-1.

show abstract

Identification of porcine alveolar macrophage glycoproteins involved in infection of porcine respiratory and reproductive syndrome virus

Wissink¹,

Wijk²,

Pol³

et al. 2003

Arch Virol

View full text Add to dashboard Cite

The aim of this study was to identify the receptor(s) for PRRSV on porcine alveolar macrophages (PAMs) by producing monoclonal antibodies (MAbs) against these cells. Hybridoma supernatants were selected for their ability to block PRRSV infection. Four MAbs, 1-8D2, 9.4C7, 9.9F2, and 3-3H2 inhibited infection and recognised cell surface, PAM-specific antigens as shown by immunofluorescence and immunoperoxidase monolayer assay. These MAbs were then used to identify cellular proteins involved in PRRSV infection by radioimmunoprecipitation assays (RIPAs). MAbs 1-8D2 and 9.9F2 each recognised a 150 kDa-polypeptide doublet, while MAbs 9.4C7 and 3-3H2 both recognised a 220 kDa-polypeptide. Glycosidase treatment demonstrated all these polypeptides to be N-glycosylated. Thus, multiple glycoproteins appear to be involved in infection of PAMs by PRRSV.

show abstract

Effect of heparin on a haemagglutinin of porcine reproductive and respiratory syndrome virus

Cited by 33 publications

References 16 publications

Effect of Heparin on Hemagglutination by Equine Arteritis Virus.

Effect of Heparin on Hemagglutination by Equine Arteritis Virus.

Glycosaminoglycan-Binding Ability Is a Feature of Wild-Type Strains of Herpes Simplex Virus Type 1

Identification of porcine alveolar macrophage glycoproteins involved in infection of porcine respiratory and reproductive syndrome virus

Contact Info

Product

Resources

About