Effect of Hexachlorocyclohexane (HCH), Its Isomers, and Metabolites on Hsp70 Expression in TransgenicDrosophila melanogaster

Chowdhuri, D. Kar; Saxena, D. K.; Viswanathan, P. N.

doi:10.1006/pest.1998.2390

Cited by 49 publications

(37 citation statements)

References 48 publications

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“…Since Hsp70 expression has been shown previously to provide a direct measure of cytotoxicity [12][13][14]23,30], our results showing Hsp70 expression in the testes of the male fly further consolidated the above possibility. Interestingly, at the highest concentation of argemone oil, female flies were also found to be equally sensitive like their male partners.…”

Section: Discussionsupporting

confidence: 86%

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Argemone oil induced cellular damage in the reproductive tissues of transgenic Drosophila melanogaster: Protective role of 70 kDa heat shock protein

Mukhopadhyay¹,

Saxena²,

Bajpai³

et al. 2003

J Biochem & Molecular Tox

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We explored the reproductive toxicity of argemone oil and its principal alkaloid fraction in transgenic Drosophila melanogaster (hsp70-lacZ) Bg(9). The toxicity of argemone oil has been attributed to two of its physiologically active benzophenanthridine alkaloids, sanguinarine and dihydrosanguinarine. Freshly eclosed first instar larvae of transgenic Drosophila melanogaster were transferred to different concentrations of argemone oil and its alkaloid fraction contaminated food. Virgin flies that eclosed from the contaminated food were pair-mated to look into the effect on reproduction. The study was further extended by investigating hsp70 expression and tissue damage in larval gonads, genital discs, and reproductive organs of adult fly. Our results showed that argemone oil was more cytotoxic than its principal alkaloid fraction. Moreover, it was the male fly that was more affected compared to its opposite number. The accessory glands of male reproductive system of the fly, which did not express hsp70, exhibited severe damage as evidenced by Trypan blue staining. This prompted us to explore the ultrastructural morphology of the gland, which showed acute signs of necrosis in both the cell types as evident by necrotic nuclei, higher vacuolization, and disorganized endoplasmic reticulum, decrease in the number of Golgi vesicles and disorganized, loosely packed filamentous structures in the lumen of the accessory gland, at the higher concentrations of the adulterant. The study showed the reproductive toxicity of argemone oil and its alkaloid fraction in transgenic Drosophila melanogaster and further confirmed the cytoprotective role of hsp70.

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Section: Discussionsupporting

confidence: 86%

“…In situ X-gal staining was performed using the method of O'Kane and Gehring [22] with some minor modifications [23]. Briefly, male and female gonads and genital discs were dissected out in Poels' salt solution (PSS) [24] from late third instar larvae (100 ± 2 h) of control and treated groups.…”

Section: In Larval Gonads and Genital Discsmentioning

confidence: 99%

Argemone oil induced cellular damage in the reproductive tissues of transgenic Drosophila melanogaster: Protective role of 70 kDa heat shock protein

Mukhopadhyay¹,

Saxena²,

Bajpai³

et al. 2003

J Biochem & Molecular Tox

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“…For qualitative assay, the method of O' Kane and Gehring (1987) was used with modifications (Kar Chowdhuri et al, 1999). 50-55 larval tissues were explanted in PSS after removal of their cuticle.…”

Section: Stress Gene Assaymentioning

confidence: 99%

“…The model falls within the recommendation of the European Centre for the Validation of Alternative Methods (ECVAM) whose goal is to promote the scientific and regulatory acceptance of alternative methods (Benford et al, 2000). In the past decade, the model has been used for elucidating human disease (Bier, 2005) and toxicological studies (Wasserkort and Koller, 1997;Kar Chowdhuri et al, 1999;Mukhopadhyay et al, 2003;Siddique et al, 2005aSiddique et al, , 2005bNazir et al, 2006).…”

Section: Introductionmentioning

confidence: 97%

Adverse effect of tannery waste leachates in transgenic Drosophila melanogaster: role of ROS in modulation of Hsp70, oxidative stress and apoptosis

Siddique

Gupta

Mitra

et al. 2008

J of Applied Toxicology

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Leachate is a complex chemical mixture of chemicals produced as a result of leaching of solid wastes. The potential toxicity of leachates is a major environmental health concern. The present study evaluated the role of ROS in tannery leachates induced Hsp70 expression, antioxidant enzymes and apoptosis in Drosophila. Different concentrations (0.05-2.0%) of leachates prepared from tannery waste at different pH (7.00, 4.93 and 2.88) were mixed with Drosophila food and fed to the larvae for 2-48 h to examine the different stress and apoptotic markers. A concentration- and time-dependent significant increase in Hsp70 expression, ROS generation, antioxidant enzymes activities and MDA content were observed in the exposed larvae. Activities of antioxidant enzymes were delayed compared with Hsp70 expression and MDA level in the exposed organisms. Apoptotic cell death was observed in the exposed larvae at higher concentrations concurrent with a significant regression in Hsp70 along with a higher level of ROS generation. A positive correlation drawn between ROS generation and apoptotic markers and a negative correlation between apoptotic markers and Hsp70 expression at these concentrations indicated the important role of ROS in the induction of cellular damage in the exposed organisms. There was a significant generation of ROS in the larvae exposed to 0.5% of leachates which did not interfere with the protection of their cells by Hsp70 and antioxidant enzymes. However, generation of significantly higher levels of ROS in the larvae exposed to 1.0% and 2.0% leachates may decrease Hsp70 expression thus leading to mitochondria-mediated caspase-dependent apoptotic cell death.

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“…Jowett [81,82] showed that transgenic fruit fly could be used to study the drug metabolism as well as oxidative stress. Kar Chowdhuri et al [83] have reported hsp70 expression as a measure of cytotoxicity against hexachlorohexane, its isomers, and metabolites in transgenic Drosophila melanogaster (hsp70-lacZ)Bg 9 . Transgenic Drosophila melanogaster line that expresses bacterial ␤-galactosidase as a response to stress was used during the study [84].…”

Section: Transgenic Animal Models For Hsp70mentioning

confidence: 99%

Heat shock response: hsp70 in environmental monitoring

Mukhopadhyay

Nazir

Saxena

et al. 2003

J Biochem & Molecular Tox

146

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Heat shock proteins (Hsps) are a ubiquitous feature of cells in which these proteins cope with stress-induced denaturation of other proteins. Among the different families of Hsps, the 70 kDa family (hsp70) is the most highly conserved and has been most extensively studied. Apart from their primary role in cellular defense under stress condition, a number of studies in recent years have shown the immense potential of hsp70 in pollution monitoring using even transgenic approach both in vivo and in vitro. This article reviews the recent developments in the widespread application of hsp70 in environmental risk assessment. C

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Effect of Hexachlorocyclohexane (HCH), Its Isomers, and Metabolites on Hsp70 Expression in TransgenicDrosophila melanogaster

Cited by 49 publications

References 48 publications

Argemone oil induced cellular damage in the reproductive tissues of transgenic Drosophila melanogaster: Protective role of 70 kDa heat shock protein

Argemone oil induced cellular damage in the reproductive tissues of transgenic Drosophila melanogaster: Protective role of 70 kDa heat shock protein

Adverse effect of tannery waste leachates in transgenic Drosophila melanogaster: role of ROS in modulation of Hsp70, oxidative stress and apoptosis

Heat shock response: hsp70 in environmental monitoring

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