Effect of hierarchical porous scaffold on osteoimmunomodulation and bone formation

Liu, Yang; Cao, Lingyan; Zhang, Shuang; Ji, Luli; Wang, Jing; Liu, Changsheng

doi:10.1016/j.apmt.2020.100779

Cited by 15 publications

(12 citation statements)

References 44 publications

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“…[151] Triple porosity, including nanopores, can be achieved when a nanoporous filler is used in the composite scaffold. [165,166] For example, PLGA/Bg composite scaffolds with triple porosity were fabricated with macropores of diameter 300-500 μm created by SCPL using NaCl as porogen, micropores of size <10 μm created by TIPS, and nanopores of diameter %7.5 nm present in the sol-gel-derived Bg filler particles. [165] 3.1.4.…”

Section: Tipsmentioning

confidence: 99%

“…[165,166] For example, PLGA/Bg composite scaffolds with triple porosity were fabricated with macropores of diameter 300-500 μm created by SCPL using NaCl as porogen, micropores of size <10 μm created by TIPS, and nanopores of diameter %7.5 nm present in the sol-gel-derived Bg filler particles. [165] 3.1.4. SCPL and Emulsion Templating SCPL has also been combined with emulsion templating to introduce dual porosity in scaffolds.…”

Section: Tipsmentioning

confidence: 99%

“…[154,188,190,191,222,243,244] And finally, a few studies also performed in vivo tests on scaffolds with dual and triple porosity prepared by SCPL, 3D printing, and TIPS-based techniques. [132,146,165,182,189,192,193,201,245,246] Of all studies on the biological activity of scaffolds with multiscale porosity, only a few directly compared the biological activity of scaffolds with a single pore type with the activity of scaffolds with dual or triple porosity. [29,30,42,44,65,66,68,97,134,166] The results obtained by these studies are summarized in Table 1 and exemplified in Figure 6.…”

Section: Biological Activitymentioning

confidence: 99%

“…[ 154,188,190,191,222,243,244 ] And finally, a few studies also performed in vivo tests on scaffolds with dual and triple porosity prepared by SCPL, 3D printing, and TIPS‐based techniques. [ 132,146,165,182,189,192,193,201,245,246 ]…”

Section: Fabrication Of Scaffolds With Multiscale Porositymentioning

confidence: 99%

See 3 more Smart Citations

Glass, Ceramic, Polymeric, and Composite Scaffolds with Multiscale Porosity for Bone Tissue Engineering

Jeyachandran

Cerruti

2023

Adv Eng Mater

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Porosity affects performance of scaffolds for bone tissue engineering both in vitro and in vivo. Macropores (i.e., pores with a diameter >100 μm) are essential for cellular infiltration; micropores (i.e., pores with a diameter of 1–10 μm) promote cell adhesion and facilitate nutrient absorption. Scaffolds containing both macropores and micropores exploit the advantages of both pore sizes and have excellent osteogenic properties. Nanopores (i.e., pores with a diameter of 1–50 nm) can be included as well, to improve cell–material interactions by further enhancing the surface area of the scaffold. This article reviews fabrication techniques and properties of scaffolds with multiscale porosity, focusing on glass, ceramic, polymeric, and composite scaffolds. After discussing the structure of bone and how it inspired scaffolds for bone tissue engineering, pore nomenclature is introduced. Then, the techniques used to induce multiscale porosity, the nature of the pores created, and the effects of scaffold porosity on mechanical properties and biological activity of the scaffolds are discussed. The review concludes by providing an outlook for this field, including advancements that are made possible by computational modeling and artificial intelligence.

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Section: Tipsmentioning

confidence: 99%

Section: Tipsmentioning

confidence: 99%

Section: Biological Activitymentioning

confidence: 99%

Section: Fabrication Of Scaffolds With Multiscale Porositymentioning

confidence: 99%

See 2 more Smart Citations

Glass, Ceramic, Polymeric, and Composite Scaffolds with Multiscale Porosity for Bone Tissue Engineering

Jeyachandran

Cerruti

2023

Adv Eng Mater

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“…The morphological characteristics of materials, especially the design of surface with micro/nano-morphological features is one of the valuable strategies to improve the biological function of materials [ 20 , 21 ]. For example,poly (lactic-co-glycolic acid) (PLGA) and bioactive glass-based scaffolds with macro–micro–meso pores could increase M2 polarization, and enhance the angiogenesis and osteogenic performance compared with the scaffolds with macro or macro–meso pores [ 23 ]. The 3D porous structure on the sulfonated PEEK could reduce infiltration of inflammatory cells compared with PEEK [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

Hydroxyapatite composited PEEK with 3D porous surface enhances osteoblast differentiation through mediating NO by macrophage

Liu

Ouyang

Chen

et al. 2021

Regenerative Biomaterials

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The adverse immune response mediated by macrophages is one of the main factors that are prone to lead poor osseointegration of polyetheretherketone (PEEK) implants in clinic. Hence, endowing PEEK with immunomodulatory ability to avoid the adverse immune response becomes a promise strategy to promote bone repair. In this work, sulfonation and hydrothermal treatment were used to fabricate a three-dimensional porous surface on PEEK and hydroxyapatite composited PEEK. The hydroxyapatite composited PEEK with three-dimensional porous surface inhibited macrophages polarizing to M1 phenotype and down-regulated iNOS protein expression, which led to a nitric oxide concentration reduction in culture medium of mouse bone marrow mesenchymal stem cells (mBMSCs) under co-culture condition. The decrease of nitric oxide concentration could help to increase bone formation related OSX and ALP genes expressions and decrease bone resorption related MMP-9 and MMP-13 genes expressions via cAMP-PKA-RUNX2 pathway in mBMSCs. In summary, the hydroxyapatite composited PEEK with three-dimensional porous surface has the potential to promote osteogenesis of PEEK through immunomodulation, which provides a promising strategy to improve the bone repair ability of PEEK.

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An Approach for Fabricating Hierarchically Porous Cell‐Laden Constructs Utilizing a Highly Porous Collagen‐Bioink

Koo,

Kim

2024

Adv Funct Materials

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In bioprinting procedures, bioinks typically comprise hydrogels, necessitating a balance between printability and bioactivity. Achieving good printability requires a relatively high hydrogel viscosity, but this can reduce bioactivity due to poor cell‐to‐cell interactions of the encapsulated cells. Therefore, developing hydrogels with excellent printability and bioactivity is a significant challenge in bioprinting. Here, a collagen‐based bioink with excellent printability and bioactivity is developed. The bioink is prepared through a two‐step process: first, by subjecting the bioink to whipping to create a non‐homogeneous porous structure, and subsequently, by employing centrifugation to refine this porous structure into a uniform and relatively smaller microscale porous structure (with a specific diameter of 15.7 ± 9.2 µm). Using the designed bioink, a hierarchical porous structure is fabricated without any special equipment or supporting/sacrificing materials. To demonstrate the feasibility of the collagen bioink laden with human adipose stem cells, a hierarchical cell construct is fabricated using a bioprinter. The construct exhibits significantly enhanced cellular activities and superior osteogenic differentiation compared to the bioprinted cell constructs using normal collagen bioink. These findings suggest that collagen‐based bioinks, which are both printable and bioactive, hold significant promise for extensive utilization in a variety of tissue regeneration applications.

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Effect of hierarchical porous scaffold on osteoimmunomodulation and bone formation

Cited by 15 publications

References 44 publications

Glass, Ceramic, Polymeric, and Composite Scaffolds with Multiscale Porosity for Bone Tissue Engineering

Glass, Ceramic, Polymeric, and Composite Scaffolds with Multiscale Porosity for Bone Tissue Engineering

Hydroxyapatite composited PEEK with 3D porous surface enhances osteoblast differentiation through mediating NO by macrophage

An Approach for Fabricating Hierarchically Porous Cell‐Laden Constructs Utilizing a Highly Porous Collagen‐Bioink

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