Effect of high-fat diet on lypolisis in isolated adipocytes from visceral and subcutaneous WAT

Abstract

Variations in total energy intake and composition of daily food play an important role in the regulation of metabolic processes and so, in the control of body weight. This study was designed in order to investigate the effect of a high-fat diet on lipolysis in isolated adipocytes. For this purpose, fourteen Wistar rats were divided into two groups and fed either a standard-fat diet or a high-fat diet ad libitum for 7 weeks. Adipocytes were prepared from fat pads by collagenase digestion and incubated in vitro in the absence or presence of various lipolytic agents. Lipolysis was measured by the release of glycerol into the medium during 90 min of incubation. We observed that a high amount of fat in the diet induced an enlargement of adipose tissue, which was accompanied by a reduction of beta-adrenergic agonist-induced lipolysis, that could be due to a loss of beta(1) and beta(3)-adrenoceptor number or to alterations of their coupling to adenylate-cyclase through the guanine nucleotide regulatory protein. New data about regional differences were provided by comparing two adipose locations (subcutaneous and visceral).

Time-Dependent Effects of a High-Energy-Yielding Diet on the Regulation of Specific White Adipose Tissue Genes

“…High-fat feeding was used to generate diet induced obesity as previously described (23,24). Rats eating such a diet, increased energy intake, body weight and adiposity, which is in good accordance with previous data (25).…”

Reduction of fat storage in mice fed a high‐fat diet long term by treatment with saponins prepared from Kochia scoparia fruit

“…Obesity is closely associated with life-style-related diseases such as hyperlipidemia, hypertension, atherosclerosis and noninsulin-dependent diabetes mellitus and with an increased risk of coronary heart disease (Leonhardt et al, 1999). Dietary fat can increase body weight and adiposity in humans and animals more effectively than dietary carbohydrate (Han et al, 1999a;Portillo et al, 1999). However, the pharmacological actions of K. scoparia fruit on life-style-related diseases have not been clarified.…”

Tea catechins enhance the mRNA expression of uncoupling protein 1 in rat brown adipose tissue

“…Table 1 details the composition of the experimental diets used in the present study. These diets were prepared as described previously [21] in accordance with the recommendations of the American Institute of Nutrition. The HF diet contained 30 g coconut oil/100 g chow as a fat source, in addition to 5 g olive oil/100 g chow.…”