Effect of high ferric ion concentrations on total lipids and lipid characteristics of Tetraselmis subcordiformis, Nannochloropsis oculata and Pavlova viridis

Huang, Xuxiong; Wei, Likun; Huang, Zhengzheng; Yan, Jiaqi

doi:10.1007/s10811-013-0056-x

Cited by 30 publications

(19 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…oculata has a lipid content of about 25% (DW) [33]. This study and our high irradiation experiment (unpublished) found that both high light and nitrogen deficiency (more likely) collaboratively enhanced the lipid accumulation in N .…”

Section: Discussionmentioning

confidence: 63%

“…Thus, the amassing of TAGs is a mechanism used by some microalgae to adapt to high light stress [25, 36–38]. Many studies show that a high concentration of iron ions can promote not only the efficiencies of chlorophyll synthesis and photosynthesis but also lipid accumulation [14, 33, 39]. According to the nitrogen-iron-temperature combined culture experiment, N .…”

Section: Discussionmentioning

confidence: 99%

“…oculata under 6.72 mg Fe·L -1 had a high TL content (53%) and the highest lipid production (142 mg·L -1 ) [14]. There are direct action sites for iron in the electron transport chains in chloroplasts and mitochondria, which affect photosynthesis and respiration, thus affecting carbon storage and lipid reserves [33, 39]. …”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Long-duration effect of multi-factor stresses on the cellular biochemistry, oil-yielding performance and morphology of Nannochloropsis oculata

Wei

Huang

2017

PLoS ONE

Self Cite

View full text Add to dashboard Cite

Microalga Nannochloropsis oculata is a promising alternative feedstock for biodiesel. Elevating its oil-yielding capacity is conducive to cost-saving biodiesel production. However, the regulatory processes of multi-factor collaborative stresses (MFCS) on the oil-yielding performance of N. oculata are unclear. The duration effects of MFCS (high irradiation, nitrogen deficiency and elevated iron supplementation) on N. oculata were investigated in an 18-d batch culture. Despite the reduction in cell division, the biomass concentration increased, resulting from the large accumulation of the carbon/energy-reservoir. However, different storage forms were found in different cellular storage compounds, and both the protein content and pigment composition swiftly and drastically changed. The analysis of four biodiesel properties using pertinent empirical equations indicated their progressive effective improvement in lipid classes and fatty acid composition. The variation curve of neutral lipid productivity was monitored with fluorescent Nile red and was closely correlated to the results from conventional methods. In addition, a series of changes in the organelles (e.g., chloroplast, lipid body and vacuole) and cell shape, dependent on the stress duration, were observed by TEM and LSCM. These changes presumably played an important role in the acclimation of N. oculata to MFCS and accordingly improved its oil-yielding performance.

show abstract

Section: Discussionmentioning

confidence: 63%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Long-duration effect of multi-factor stresses on the cellular biochemistry, oil-yielding performance and morphology of Nannochloropsis oculata

Wei

Huang

2017

PLoS ONE

Self Cite

View full text Add to dashboard Cite

show abstract

“…The effect of optimum iron concentration on microalgae growth was reported by Huang et al (2014). Kadar et al (2012) compared commonly used Fe-EDTA with three nZVI forms with distinct surface properties.…”

Section: Introductionmentioning

confidence: 99%

Trace concentrations of iron nanoparticles cause overproduction of biomass and lipids during cultivation of cyanobacteria and microalgae

et al. 2014

View full text Add to dashboard Cite

Cultivation in Zehnder medium containing 5.1 mg L −1 zero-valent iron nanoparticles (nZVI) boosted the growth of the green algae Desmodesmus subspicatus, Dunaliella salina, Parachlorella kessleri and Raphidocelis subcapitata and the eustigmatophycean algae Nannochloropsis limnetica and Trachydiscus minutus. In the cyanobacterium Arthrospira maxima, growth stimulation occurred at 1.7-5.1 mg L −1 nZVI. In all studied microorganisms, 5.1 mg L −1 nZVI strongly enhanced lipid accumulation, decreased the content of saturated and monounsaturated fatty acids with the exception of palmitoleic acid and increased the content of polyunsaturated fatty acids in cells. The nZVI particles may provide a suitable source of iron causing increased cell growth and induce metabolic changes resulting in higher lipid production and changes in fatty acid (FA) composition. Altered lipid synthesis may reflect the oxidative action of nZVI. Further research may contribute to optimizing the economical production of oils from oleaginous microorganisms and help clarify the mechanism of nZVI action.

show abstract

“…Thus, this species is considered to be an essential source of PUFAs, especially eicosapentaenoic acid (EPA) [32]. According to Huang et al [33], total lipid content of microalgae Tetraselmis subcordiformis reached up 33.72% of dry weight (DW) when it was cultivated in presence of 1.2 mM ferric ion. Marine green microalgae species such as Nannochloropsis sp.…”

Section: Introductionmentioning

confidence: 99%

Enhanced lipid and biomass production by a newly isolated and identified marine microalga

et al. 2016

View full text Add to dashboard Cite

BackgroundThe increasing demand for microalgae lipids as an alternative to fish has encouraged researchers to explore oleaginous microalgae for food uses. In this context, optimization of growth and lipid production by the marine oleaginous V2-strain-microalgae is of great interest as it contains large amounts of mono-unsaturated (MUFAs) and poly-unsaturated fatty acids (PUFAs).MethodsIn this study, the isolated V2 strain was identified based on 23S rRNA gene. Growth and lipid production conditions were optimized by using the response surface methodology in order to maximize its cell growth and lipid content that was quantified by both flow cytometry and the gravimetric method. The intracellular lipid bodies were detected after staining with Nile red by epifluorescence microscopy. The fatty acid profile of optimal culture conditions was determined by gas chromatography coupled to a flame ionization detector.ResultsThe phenotypic and phylogenetic analyses showed that the strain V2 was affiliated to Tetraselmis genus. The marine microalga is known as an interesting oleaginous species according to its high lipid production and its fatty acid composition. The optimization process showed that maximum cell abundance was achieved under the following conditions: pH: 7, salinity: 30 and photosynthetic light intensity (PAR): 133 μmol photons.m−2.s−1. In addition, the highest lipid content (49 ± 2.1% dry weight) was obtained at pH: 7, salinity: 37.23 and photosynthetic light intensity (PAR): 188 μmol photons.m−2.s−1. The fatty acid profile revealed the presence of 39.2% and 16.1% of total fatty acids of mono-unsaturated fatty acids (MUFAs) and poly-unsaturated fatty acids (PUFAs), respectively. Omega 3 (ω3), omega 6 (ω6) and omega 9 (ω9) represented 5.28%, 8.12% and 32.8% of total fatty acids, respectively.ConclusionsThis study showed the successful optimization of salinity, light intensity and pH for highest growth, lipid production and a good fatty acid composition, making strain V2 highly suitable for food and nutraceutical applications.

show abstract

Effect of high ferric ion concentrations on total lipids and lipid characteristics of Tetraselmis subcordiformis, Nannochloropsis oculata and Pavlova viridis

Cited by 30 publications

References 43 publications

Long-duration effect of multi-factor stresses on the cellular biochemistry, oil-yielding performance and morphology of Nannochloropsis oculata

Long-duration effect of multi-factor stresses on the cellular biochemistry, oil-yielding performance and morphology of Nannochloropsis oculata

Trace concentrations of iron nanoparticles cause overproduction of biomass and lipids during cultivation of cyanobacteria and microalgae

Enhanced lipid and biomass production by a newly isolated and identified marine microalga

Contact Info

Product

Resources

About