Effect of high hydrostatic pressure on the BK channel in bovine chromaffin cells

Macdonald, A. G.

doi:10.1016/s0006-3495(97)78217-1

Cited by 20 publications

(14 citation statements)

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“…The results show that this channel was modulated by stretching of the membrane as well as by the membrane voltage (depolarization) and by [Ca 2ϩ ] i . Similar results have been described in other cell types, such as skeletal muscle, 16 osteoblasts, 17,18 smooth muscle, 19,20 chromaffin, 32 and neuroepithelial cells. 21 The basic characteristics of the BK Ca channel reported in this study are in agreement with those described by Stumpff et al 13 in the same cells and in human TM cells.…”

Section: Discussionsupporting

confidence: 83%

Cell Membrane Stretch Modulates the High-Conductance Ca²⁺-Activated K⁺Channel in Bovine Trabecular Meshwork Cells

Gasull

Ferrer

Llobet

et al. 2003

Invest. Ophthalmol. Vis. Sci.

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The data show that in TM cells, open probability of the BK(Ca) channel is enhanced by membrane stretching as well as by membrane depolarization and [Ca(2+)](i). Changes in membrane tension induced by cell volume increase also activated whole-cell BK(Ca) currents. Homeostatic mechanisms in TM cells may involve BK(Ca) channel activation in response either to changes in cell volume or changes in IOP.

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Section: Discussionsupporting

confidence: 83%

Cell Membrane Stretch Modulates the High-Conductance Ca²⁺-Activated K⁺Channel in Bovine Trabecular Meshwork Cells

Gasull

Ferrer

Llobet

et al. 2003

Invest. Ophthalmol. Vis. Sci.

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“…Such changes in pattern may also be induced by Ca 2+ -activated K + currents [22]. Therefore, the effect of [Ca 2+ ] o on the Ca 2+ spike firing was examined.…”

Section: Resultsmentioning

confidence: 99%

“…Previous studies have proposed enhancement of K + currents at high pressure. For example, pressure decreased mammalian cardiac pacemaker function, probably by slowing the diastolic decay of a voltage-dependent K + conductance [24], increased the voltage sensitivity of delayed rectifier K + current in the lobster axon [9], enhanced a slow component of K + conductance in the squid giant axon [4] and increased the opening probability of the BK-type Ca 2+ -activated channel [22]. The present results suggest that although such pressure-induced enhancement of K + conductance(s) has minor effects on somatic firing of CNS neurons, its influence on dendritic excitability is quite prominent.…”

Section: Discussionmentioning

confidence: 99%

Spontaneous Na + and Ca 2+ spike firing of cerebellar Purkinje neurons at high pressure

Etzion

Grossman

1999

Pfl�gers Archiv European Journal of Physiology

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The effects of high pressure (up to 10.1 MPa) on the spontaneous firing of Purkinje neurons in guinea-pig cerebellar slices were studied using the macropatch clamp technique. Pressure did not significantly alter the single somatic Na+ spike parameters or the frequency of regular Na+ spike firing. When Na+ currents were blocked by 0.5-1 microM tetrodotoxin (TTX), a pressure of 10.1 MPa slightly reduced the dendritic Ca2+ spike amplitude to 90.2+/-3.1% of its control value, and slowed its kinetics. The effects of pressure on the single Ca2+ spike were even less prominent when K+ currents were blocked by 5 mM 4-aminopyridine (4-AP). Pressure prolonged the active period of Ca2+ spike firing to 152.2+/-10.4% of the control value. Within the active period pressure increased the inter-spike interval to 164.9+/-8.7% and suppressed the typical firing of doublets. The latter changes were reversed by a high extracellular potassium concentration ([K+]o) and 1 microM 4-AP, whereas in the presence of 5 mM 4-AP the pattern was insensitive to pressure. A high [Ca2+]o reduced the firing frequency and suppressed doublet firing in a manner reminiscent of the pressure effect, but these changes could not be reversed by 4-AP. A low [Ca2+]o slightly increased the firing of doublets. These results show that the single somatic Na+ spike is insensitive and the dendritic Ca2+ spike is only mildly sensitive to pressure. However, alterations in Ca2+ spike firing pattern suggest that modulation of dendritic K+ currents induce depression of dendritic excitability at pressure.

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“…Mid1 in Saccharomyces cerevisiae, the first eukaryotic calcium-permeable stretch-activated cation channel identified, is essential for supplying calcium ions during the mating process (24,28,34). In bovine chromaffin cells, the large-conductance Ca 2ϩ -activated K ϩ (BK) channel, which is selective for potassium ions, is known to be activated by voltage and also hydrostatic pressure (31). Fluctuation of hydrostatic pressure is commonly observed in diarthroses.…”

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confidence: 99%

Tryptophan Permease Gene TAT2 Confers High-Pressure Growth in Saccharomyces cerevisiae

Abe

Horikoshi

2000

Molecular and Cellular Biology

141

111

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Hydrostatic pressure in the range of 15 to 25 MPa was found to cause arrest of the cell cycle in G 1 phase in an exponentially growing culture of Saccharomyces cerevisiae, whereas a pressure of 50 MPa did not. We found that a plasmid carrying the TAT2 gene, which encodes a high-affinity tryptophan permease, enabled the cells to grow under conditions of pressure in the range of 15 to 25 MPa. Additionally, cells expressing the Tat2 protein at high levels became endowed with the ability to grow under low-temperature conditions at 10 or 15°C as well as at high pressure. Hydrostatic pressure significantly inhibited tryptophan uptake into the cells, and the Tat2 protein level was down-regulated by high pressure. The activation volume associated with tryptophan uptake was found to be a large positive value, 46.2 ؎ 3.85 ml/mol, indicating that there was a net volume increase in a rate-limiting step in tryptophan import. The results showing cell cycle arrest in G 1 phase and down-regulation of the Tat2 protein seem to be similar to those observed upon treatment of cells with the immunosuppressive drug rapamycin. Although rapamycin treatment elicited the rapid dephosphorylation of Npr1 and induction of Gap1 expression, hydrostatic pressure did not affect the phosphorylation state of Npr1 and it decreased the level of Gap1 protein, suggesting that the pressure-sensing pathway may be independent of Npr1 function. Here we describe high-pressure sensing in yeast in comparison with the TOR-signaling pathway and discuss an important factor involved in adaptation of organisms to high-pressure environments.

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Effect of high hydrostatic pressure on the BK channel in bovine chromaffin cells

Cited by 20 publications

References 50 publications

Cell Membrane Stretch Modulates the High-Conductance Ca²⁺-Activated K⁺Channel in Bovine Trabecular Meshwork Cells

Cell Membrane Stretch Modulates the High-Conductance Ca²⁺-Activated K⁺Channel in Bovine Trabecular Meshwork Cells

Spontaneous Na + and Ca 2+ spike firing of cerebellar Purkinje neurons at high pressure

Tryptophan Permease Gene TAT2 Confers High-Pressure Growth in Saccharomyces cerevisiae

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Effect of high hydrostatic pressure on the BK channel in bovine chromaffin cells

Cited by 20 publications

References 50 publications

Cell Membrane Stretch Modulates the High-Conductance Ca2+-Activated K+Channel in Bovine Trabecular Meshwork Cells

Cell Membrane Stretch Modulates the High-Conductance Ca2+-Activated K+Channel in Bovine Trabecular Meshwork Cells

Spontaneous Na + and Ca 2+ spike firing of cerebellar Purkinje neurons at high pressure

Tryptophan Permease Gene TAT2 Confers High-Pressure Growth in Saccharomyces cerevisiae

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Cell Membrane Stretch Modulates the High-Conductance Ca²⁺-Activated K⁺Channel in Bovine Trabecular Meshwork Cells

Cell Membrane Stretch Modulates the High-Conductance Ca²⁺-Activated K⁺Channel in Bovine Trabecular Meshwork Cells