Effect of high intratesticular estrogen on the seminiferous epithelium in adult male rats

D’Souza, Ryan S; Gill-Sharma, M. K.; Pathak, Sulabha; Kedia, Neelam; Kumar, R. Asok; Balasinor, Nafisa

doi:10.1016/j.mce.2005.04.011

Cited by 95 publications

(91 citation statements)

References 22 publications

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“…At adulthood, the activation of male sexual behavior depends on testosterone circulating levels, its effects being mediated by androgen receptors in specific hypothalamic nuclei (McGinnis et al, 1996;Heart et al, 2004;D'Souza et al). In the present experiment, we observed that T levels were reduced lower in adult male rats after the post-weaning period by the effect of estrogen administered paranterally (PPD group).…”

Section: Discussionsupporting

confidence: 51%

“…Estrogen has been shown to have a definitive role in spermatogenesis, but the molecular events directly regulated by it have remained enigmatic (D'Souza et al, 2005). It has been suggested that rats exposed to estrogen during the neonatal period show permanent damage in the adult male reproductive organs, such as a decrease in testicular weight (Vigueras-Villasenor et al).…”

Section: Discussionmentioning

confidence: 99%

“…According to our results, the lowest testosterone level was observed in the PPD group. Researchers suggest high intratesticular estradiol with a concomitant decrease in intratesticular testosterone levels on spermatogenesis (D'Souza et al;Della Seta et al, 2006). The results of our study are consistent with those reported in these earlier studies.…”

Section: Discussionmentioning

confidence: 99%

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The Effects of Maternal Low Protein and Estrogen on Sexual Behavior and Testicular Maturation in Male Rats

Kavak

Balsak²,

Toğrul³

et al. 2015

Int. J. Morphol.

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SUMMARY:In recent years, disorders related to the development and function of the male reproductive tract has increased, thus generating a surprising decrease in semen volume and sperm count. We examined the effects of low protein and estrogen on sexual behavior and testicular maturation in male rats. We also examined FSH, LH and testosterone levels and histological damage of testis tissue. The male rats were subjected to standard long-term treatment with estradiol by oral and paranteral delivery. The number of mounts, copulatory efficiency and ejaculation latencies for the paranteral protein diet (PPD) group was significantly lower than those in a group nourished with a low protein diet (LPD) and oral protein diet (OPD) groups (P<0.05). Testes and epididymis sections were examined by four grades, according to the level of damage of epithelium in the testes and epididymis. Higher histological damage was also detected in the PPD group. In conclusion, the present study confirmed that unwanted estrogen effects were higher in the paranteral administered group on examination of sexual behavior and histological damage of epithelium in the testes and epididymis of male rats.

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Section: Discussionsupporting

confidence: 51%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

The Effects of Maternal Low Protein and Estrogen on Sexual Behavior and Testicular Maturation in Male Rats

Kavak

Balsak²,

Toğrul³

et al. 2015

Int. J. Morphol.

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show abstract

“…Caudal sperm counts were affected by all doses of PPT treatment and lower doses of DPN treatment ( Fig. 2D and E) which could be due to reduced sperm production in the testis, apoptosis of germ cells or retention of spermatids in the seminiferous epithelium, all of which are typical effects of E 2 exposure (D'Souza et al 2005). The exact reasons for the low counts are currently being investigated.…”

Section: Discussionmentioning

confidence: 97%

Effect of estrogen receptor-subtype-specific ligands on fertility in adult male rats

Dumasia

Kumar

Kadam

et al. 2015

Journal of Endocrinology

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Maintenance of normal male fertility relies on the process of spermatogenesis which is under complex endocrine control by mechanisms involving gonadotropin and steroid hormones. Although testosterone is the primary sex steroid in males, estrogen is locally produced in the testis and plays a very crucial role in male fertility. This is evident from presence of both the estrogen receptors alpha (ERa) and beta (ERb) in the testis and their absence, as in the case of knockout mice models, leads to sterility. The present study was undertaken to understand individual roles of the two ERs in spermatogenesis and their direct contribution towards the maintenance of male fertility using receptor-subtype-specific ligands. Administration of ERa and b agonists to adult male rats for 60 days results in a significant decrease in fertility, mainly due to an increase in pre-and post-implantation loss and a concomitant decrease in litter size and sperm counts. Our results indicate that ERa is mainly involved in negative feedback regulation of gonadotropin hormones, whereas both ERs are involved in regulation of prolactin and testosterone production. Histological examinations of the testis reveal that ERb could be involved in the process of spermiation since many failed spermatids were observed in stages IX-XI following ERb agonist treatment. Our results indicate that overactivation of estrogen signaling through either of its receptors can have detrimental effects on the fertility parameters and that the two ERs have both overlapping and distinct roles in maintenance of male fertility.

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“…The administration of estradiol to adult rats or mice affects spermatogenesis (47)(48)(49). At first glance, this suggests that high estradiol concentrations may contribute to spermiogenesis defects in Celf1 Ϫ/Ϫ males.…”

Section: Discussionmentioning

confidence: 99%

Hypogonadism Associated with Cyp19a1 (Aromatase) Posttranscriptional Upregulation in Celf1 Knockout Mice

Boulanger

Cibois

Viet

et al. 2015

Molecular and Cellular Biology

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CELF1 is a multifunctional RNA-binding protein that controls several aspects of RNA fate. The targeted disruption of the Celf1 gene in mice causes male infertility due to impaired spermiogenesis, the postmeiotic differentiation of male gametes. Here, we investigated the molecular reasons that underlie this testicular phenotype. By measuring sex hormone levels, we detected low concentrations of testosterone in Celf1-null mice. We investigated the effect of Celf1 disruption on the expression levels of steroidogenic enzyme genes, and we observed that Cyp19a1 was upregulated. Cyp19a1 encodes aromatase, which transforms testosterone into estradiol. Administration of testosterone or the aromatase inhibitor letrozole partly rescued the spermiogenesis defects, indicating that a lack of testosterone associated with excessive aromatase contributes to the testicular phenotype. In vivo and in vitro interaction assays demonstrated that CELF1 binds to Cyp19a1 mRNA, and reporter assays supported the conclusion that CELF1 directly represses Cyp19a1 translation. We conclude that CELF1 downregulates Cyp19a1 (Aromatase) posttranscriptionally to achieve high concentrations of testosterone compatible with spermiogenesis completion. We discuss the implications of these findings with respect to reproductive defects in men, including patients suffering from isolated hypogonadotropic hypogonadism and myotonic dystrophy type I.

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Effect of high intratesticular estrogen on the seminiferous epithelium in adult male rats

Cited by 95 publications

References 22 publications

The Effects of Maternal Low Protein and Estrogen on Sexual Behavior and Testicular Maturation in Male Rats

The Effects of Maternal Low Protein and Estrogen on Sexual Behavior and Testicular Maturation in Male Rats

Effect of estrogen receptor-subtype-specific ligands on fertility in adult male rats

Hypogonadism Associated with Cyp19a1 (Aromatase) Posttranscriptional Upregulation in Celf1 Knockout Mice

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