Effect of high night temperature on storage lipids and transcriptome changes in developing seeds of oilseed rape

Zhou, Longhua; Yan, Tao; Chen, Xin; Li, Zhilan; Wu, Dezhi; Hua, Shuijin; Jiang, Lixi

doi:10.1093/jxb/ery004

Cited by 38 publications

(39 citation statements)

References 49 publications

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“…Using 10 years of field data and following a modelling approach, Baux et al (2013) observed an increase in oleic acid (C18:1) in conventional oilseed rape and high-oleic low-linolenic (HOLL) varieties associated with minimum temperature, coincided by a decrease in linoleic acid (C18:2) and linolenic acids (C18:3) concentration. In contrast, Zhou et al (2018) reported that HNT significantly decreased the total fatty acids and relative proportions of C18:0, C18:1, C20:1, in seeds of both low and high oil concentration cultivars but increased the proportions of C18:2 and C18:3 in both cultivars under HNT (19°C) compared to low night (9°C) temperatures. However, findings from our study revealed increased levels of saturated (C16:0 and C18:0) fatty acids without significant changes in unsaturated fatty acids (C18:1, C18:2, C18:3) except for C20:1 (Table 7).…”

Section: Hnt Alters Saturated Fatty Acids But Not Unsaturated Fattymentioning

confidence: 82%

High night‐time temperature during flowering and pod filling affects flower opening, yield and seed fatty acid composition in canola

Pokharel

Chiluwal

Stamm

et al. 2020

J Agronomy Crop Science

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Winter canola (Brassica napus L.) is highly sensitive to increasing temperatures during the reproductive and pod‐filling stages. Although the impact of high day‐time temperature stress on yield and quality has been documented in canola, similar information under high night‐time temperature (HNT) stress is not available. Using six hybrids and four open‐pollinated cultivars, we observed a marked shift in peak flowering towards earlier, cooler hours of the morning under HNT. Averaged across two independent experiments, the photochemical efficiency of photosystem II was significantly decreased (3%), with a significant increase in thylakoid membrane damage (13%) in the leaves of susceptible cultivars under HNT stress. Similarly, the susceptible cultivars also recorded significant reduction in biomass (34%), pod number (22%), pod weight (37%) and total seed weight (40%) per plant while the same set of agronomic traits were not affected among the tolerant cultivars. Quantitative impact of heat stress was confirmed with increased sensitivity to HNT exposure from gametogenesis until maturity resulting in a significantly higher yield loss compared to stress exposure from post‐flowering till maturity. HNT significantly decreased oil concentration, but increased protein concentration and saturated fatty acid levels in seeds of the susceptible cultivars. However, HNT had no impact on the unsaturated fatty acids in both hybrids and the open‐pollinated cultivars. Breeding targets based on fatty acid composition for enhancing canola seed quality may not be easily amenable due to the inconsistency documented with the compositional changes under heat stress. In summary, our findings conclude that canola hybrids are better suited to regions experiencing heat stress, compared to open‐pollinated cultivars, indicating the possibility of a complete shift to hybrid canola cultivation under predicted hotter climates in the future.

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Section: Hnt Alters Saturated Fatty Acids But Not Unsaturated Fattymentioning

confidence: 82%

High night‐time temperature during flowering and pod filling affects flower opening, yield and seed fatty acid composition in canola

Pokharel

Chiluwal

Stamm

et al. 2020

J Agronomy Crop Science

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“…The glyoxylate cycle could influence the final lipid accumulation in the plant glyoxysome via peroxidation. Under other abiotic stresses, glyoxylate metabolism in the seeds of different B. napus lines was also found to change (Zhou et al ., ). The results of this study suggest that VOC plays a role in reducing ROS, which can lead to enhanced drought tolerance and lipid accumulation in B. napus .…”

Section: Discussionmentioning

confidence: 97%

Drought‐responsive genes, late embryogenesis abundant group3 (LEA3) and vicinal oxygen chelate, function in lipid accumulation in Brassica napus and Arabidopsis mainly via enhancing photosynthetic efficiency and reducing ROS

Kang

Gan

et al. 2019

Plant Biotechnology Journal

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Summary Drought is an abiotic stress that affects plant growth, and lipids are the main economic factor in the agricultural production of oil crops. However, the molecular mechanisms of drought response function in lipid metabolism remain little known. In this study, overexpression (OE) of different copies of the drought response genes LEA3 and VOC enhanced both drought tolerance and oil content in Brassica napus and Arabidopsis. Meanwhile, seed size, membrane stability and seed weight were also improved in OE lines. In contrast, oil content and drought tolerance were decreased in the AtLEA3 mutant (atlea3) and AtVOC‐RNAi of Arabidopsis and in both BnLEA‐RNAi and BnVOC‐RNAi B. napus RNAi lines. Hybrids between two lines with increased or reduced expression (LEA3‐OE with VOC‐OE, atlea3 with AtVOC‐RNAi) showed corresponding stronger trends in drought tolerance and lipid metabolism. Comparative transcriptomic analysis revealed the mechanisms of drought response gene function in lipid accumulation and drought tolerance. Gene networks involved in fatty acid (FA) synthesis and FA degradation were up‐ and down‐regulated in OE lines, respectively. Key genes in the photosynthetic system and reactive oxygen species (ROS) metabolism were up‐regulated in OE lines and down‐regulated in atlea3 and AtVOC‐RNAi lines, including LACS9, LIPASE1, PSAN, LOX2 and SOD1. Further analysis of photosynthetic and ROS enzymatic activities confirmed that the drought response genes LEA3 and VOC altered lipid accumulation mainly via enhancing photosynthetic efficiency and reducing ROS. The present study provides a novel way to improve lipid accumulation in plants, especially in oil production crops.

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“…Another cause could be the negative effect of high temperatures on photosynthesis and carbohydrate synthesis. Recently, Zhou et al (2018) specified that high heat stress during the night up-regulates lipases able to degrade storage lipids and fatty-acid desaturating enzymes.…”

Section: Discussionmentioning

confidence: 99%

“…In addition to the impact on seed yield and NUE of plants, environmental stresses affect seed composition. Several studies in rapeseed reported indeed that heat stress and drought decrease oil content and increase protein content of seeds ( Elferjani and Soolanayakanahally, 2018 ; Zhou et al , 2018 ). Moreover, together with the reduction of oil content, the composition of individual fatty acids in the seeds is altered under heat stress.…”

Section: Introductionmentioning

confidence: 99%

Post-flowering biotic and abiotic stresses impact nitrogen use efficiency and seed filling in Arabidopsis thaliana

Marmagne

Jasinski

Fagard

et al. 2020

Journal of Experimental Botany

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Nitrogen (N) is an essential nutrient that plants require for the synthesis of amino acids, proteins, and many other important metabolites. Plant metabolism and growth are consequently dependent on the amount of N that is assimilated and distributed from source leaves to developing sinks, such as fruits and seeds. The environmental stresses enhanced by climate change deeply influence seed yield and seed composition, and may disturb N use efficiency (NUE) in pants. We aimed to investigate plant responses to extreme climates with regard to NUE, N remobilization efficiency, and seed composition. By studying a collection of Arabidopsis genotypes showing a range of C:N ratios in seeds, we investigated the impact of different post-flowering growth conditions (control, heat, drought, low nitrate availability, induced senescence, and induced plant defense) on seed yield, N allocation in organs, NUE, and N remobilization efficiency. We analysed how post-flowering stresses could change seed filling and showed that post-flowering stresses change both the range of N and C concentrations and the C:N stoichiometry in seeds. Using a new trait, called delta seed composition, we measured the deviation in C:N stoichiometry of each genotype and revealed the genetic determinism of the C:N stoichiometry. Altogether, the results indicate that extreme climate impacts NUE dramatically in plants and generates different bottlenecks in N fluxes during seed filling.

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Effect of high night temperature on storage lipids and transcriptome changes in developing seeds of oilseed rape

Abstract: High night temperature lowers the total fatty acids of seed and increases the proportion of 18-carbon unsaturated fatty acids by enhancing gibberellin signaling and the activity of the genes involved in fatty acid catabolism.

Cited by 38 publications

References 49 publications

High night‐time temperature during flowering and pod filling affects flower opening, yield and seed fatty acid composition in canola

High night‐time temperature during flowering and pod filling affects flower opening, yield and seed fatty acid composition in canola

Drought‐responsive genes, late embryogenesis abundant group3 (LEA3) and vicinal oxygen chelate, function in lipid accumulation in Brassica napus and Arabidopsis mainly via enhancing photosynthetic efficiency and reducing ROS

Post-flowering biotic and abiotic stresses impact nitrogen use efficiency and seed filling in Arabidopsis thaliana

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