Effect of high proportion concentrate dietary on Yak jejunal structure, physiological function and protein composition during cold season

Abstract

The current study aimed to investigate the damage of long-term high concentrate diet feeding pattern on Yak jejunal structure, physiological function and protein composition during cold season. Twelve Datong male Yak (Bos grunniens) with the same age from cold season were randomly selected and slaughtered to determine Yak jejunal digestive enzyme activity, morphology and protein composition on different feeding patterns in Tibetan Plateau. The results showed that Yak jejunum digestive enzyme activity and morphology of grazing reared group were better than those in the intensively reared group. A total of 96 differentially expressed proteins were identified by label-free Mass Spectrometry (MS), which could be concluded to two predominant themes: protein structure and inflammatory response. Nine differentially expressed proteins were correlated in Yak jejunum damage in different feeding patterns. According to this research, we found that feeding pattern resulted the differences in Yak jejunum physiological function, morphology and protein composition. This fact was confirmed long-term high dietary concentrate feeding could damage the jejunum epithelial morphology and function.

Effects of Glutamine on Rumen Digestive Enzymes and the Barrier Function of the Ruminal Epithelium in Hu Lambs Fed a High-Concentrate Finishing Diet

“…The digestive enzymes’ activity is known to affect the activity of ruminal microbes; improve the digestion, absorption, and metabolism of nutrients; and maintain the rumen’s function and health. Studies have confirmed that when feed management is not correct, a high-concentrate fattening diet can reduce the activities of digestive enzymes such as lipase, trypsin, and amylase in ruminants and cause ruminal acidosis [ 20 , 21 ]. In our study, in the CON treatment group, the long-term high-concentration concentrate feeding mode will weaken the activities of pepsin and cellulase, which may be caused by the inactivation of digestive enzymes in Hu sheep caused by high-concentration feed.…”