Effect of Hormones on Incidence of Uterine Eosinophilia in Rats

Bjersing, Lars; Borglin, N. E.

doi:10.1111/apm.1964.60.1.27

Cited by 41 publications

(16 citation statements)

References 12 publications

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“…Estrogen is a known stimulator of uterine eosinophil infiltration (Bjersing and Borglin, 1964;King et al, 1981;Lee et al, 1989). Prepubertal rats (day 20 postnatal) were subcutaneously injected daily for 3 days with estradiol valerate (100 mg/100 ml; Bristol-Myers Squibb, Princeton, NJ) or vehicle (sesame oil).…”

mentioning

confidence: 99%

A simple method for the in situ detection of eosinophils

Ain

Tash

Soares

2002

Journal of Immunological Methods

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A simple and rapid method for identifying eosinophils in tissue sections is described. The assay is based on the incubation of frozen tissue sections with phenol red and the detection of cellular fluorescence. A one-to-one relationship was observed between cells exhibiting fluorescence following exposure to phenol red and eosinophils as identified by histochemical detection of eosinophil peroxidase (EPO) activity. Using the phenol red assay, eosinophils were detected in various eosinophil-infiltrated tissues, including uteri of rats from day 1 of pregnancy and uteri of prepubertal estrogen-treated rats. Intensity of the fluorescing eosinophils was dependent upon phenol red concentration and duration of incubation. The phenol red method of eosinophil identification was disrupted by co-incubation with resorcinol, an EPO inhibitor, or catalase, a hydrogen peroxide scavenger. The merits of this assay are its simplicity and compatibility with other procedures, such as histochemistry, immunocytochemistry, and in situ ligand -receptor binding. D

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confidence: 99%

A simple method for the in situ detection of eosinophils

Ain

Tash

Soares

2002

Journal of Immunological Methods

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“…A striking feature of the estrogenic response in the rat uterus is the influx of large numbers of eosinophils into the connective tissue spaces [3,8,23]. The uterine eosinophils either undergo lysis with the release of their intracellular organelles (small granules, large granules with crystalloid bodies, mitochondria, etc.)…”

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confidence: 99%

Peroxidase-mediated Antimicrobial Activity of Rat Uterine Fluid

Klebanoff

Smith²

1970

Gynecol Obstet Invest

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Uterine fluid obtained from estrogen primed rats (adult-ovari-or immature) has an antimicrobial effect on certain bacterial, fungal and viral species when combined with iodide ions and H202. Iodide ions can be replaced by bromide ions and reagent H₂O₂ by glucose and glucose oxidase or by a H₂O₂-generating microorganism such as Lactobacillus acidophilus. A heat stable, low molecular weight inhibitor or inhibitors of the uterine fluidiodide-H₂O₂ antimicrobial system also is present in uterine fluid. Reduced glutathione, cysteine, NADH, NADPH and ascorbic acid can inhibit the antimicrobial system. Uterine fluid exhibits peroxidase activity and its antimicrobial effect in the presence of iodide and H₂O₂ is believed to be due to this enzyme. The possible presence of the peroxidase, halide, H₂O₂ antimicrobial system in human vaginal or uterine secretions is considered.

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“…For example, estrogen stimulates the infiltration of eosinophils into the uterus (Bjersing & Borglin 1964, King et al 1981, Tchernitchin 1983, Lee et al 1989. This observation was confirmed by administration of exogenous estradiol (Fig.…”

Section: And H)mentioning

confidence: 56%

“…Eosinophil dynamics are precisely controlled within the uterus by the hormonal milieu, and exhibit dramatic fluctuations during the reproductive cycle (Rytomaa 1960, Bassett 1962, Ross & Klebanoff 1966, McMaster et al 1992, Jeziorska et al 1995. For example, estrogen stimulates the infiltration of eosinophils into the uterus, whereas uterine exposure to progesterone is associated with eosinophil exit and death (Bjersing & Borglin 1964, King et al 1981, Tchernitchin 1983, Lee et al 1989, Howe et al 1990). The present study suggests that since d/tPRP specifically interacts with eosinophils, this cytokine is a candidate mediator of the anti-inflammatory actions of progesterone during gestation.…”

Section: Introductionmentioning

confidence: 99%

Eosinophils are cellular targets of the novel uteroplacental heparin-binding cytokine decidual/trophoblast prolactin-related protein

Wang

Ishimura

Walia

et al. 2000

Journal of Endocrinology

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The uterus and placenta of the mouse and rat produce a member of the prolactin (PRL) family referred to as decidual/trophoblast PRL-related protein (d/tPRP). This cytokine/hormone has been hypothesized to regulate decidual cell activities needed for the establishment and maintenance of gestation. An alkaline phosphatase (AP)-tagging strategy was used to identify d/tPRP target cells. AP-d/tPRP bound to virtually all cells and tissues to which it was exposed, consistent with our earlier evidence that d/tPRP binds to heparin-containing molecules. Moreover, we found that co-incubation with heparin or pretreatment with heparitinase greatly decreased the binding of AP-d/tPRP to tissue sections. In addition, we observed that the AP-d/tPRP probe bound to the surface of Chinese hamster ovary (CHO) cells but not to heparan sulfate-deficient CHO-pgsD-677 cells. Potential unique non-heparin d/tPRP binding sites within mouse and rat uteroplacental tissues were identified by consecutively incubating sections with AP-d/tPRP followed by heparin. This strategy led to the identification of d/tPRP target cells associated with the uterus and the labyrinth zone of the chorioallantoic placenta. Within the uterus, d/tPRP specifically bound to eosinophils. d/tPRP-binding and eosinophil peroxidase activity were co-localized and showed similar patterns of distribution during the estrous cycle, pregnancy, and following hormonal manipulation. d/tPRP interactions with eosinophils were further demonstrated in the lung and intestine, with eosinophils isolated from the peritoneum, and in mice with generalized tissue eosinophilia. Collectively, these findings suggest that intercellular d/tPRP targeting is mediated through associations with heparin-containing molecules which help direct d/tPRP to specific interactions with eosinophils within the uterus and with the labyrinthine compartment of the chorioallantoic placenta.

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Effect of Hormones on Incidence of Uterine Eosinophilia in Rats

Cited by 41 publications

References 12 publications

A simple method for the in situ detection of eosinophils

A simple method for the in situ detection of eosinophils

Peroxidase-mediated Antimicrobial Activity of Rat Uterine Fluid

Eosinophils are cellular targets of the novel uteroplacental heparin-binding cytokine decidual/trophoblast prolactin-related protein

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