Effect of HPV16 L1 virus-like particles on the aggregation of non-functionalized gold nanoparticles

Palomino-Vizcaino, Giovanni; Reséndiz, Diana Gabriela Valencia; Benítez‐Hess, María Luisa; Martínez-Acuña, Natalia; Tapia-Vieyra, Juana Virginia; Bahena, Daniel; Díaz-Sánchez, Mauricio; García-González, Octavio Patricio; Alvarez-Sandoval, Brenda Arizaí; Álvarez-Salas, Luis Marat

doi:10.1016/j.bios.2017.09.001

Cited by 17 publications

(9 citation statements)

References 47 publications

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“…There may be hydrophobic interactions present. Indeed, prior literature has indicated aggregation of viruses in the presence of nonfunctionalized gold nanoparticles due to hydrophobic interactions, 72 while porous activated carbon modified with silver and copper oxide nanoparticles showed high virus inactivation. 73 Nonetheless, in the second case, the antiviral effect was due to the well-known antiviral effect of metal NPs.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Agglomeration of Viruses by Cationic Lignin Particles for Facilitated Water Purification

Rivière

Korpi

Sipponen

et al. 2020

ACS Sustainable Chem. Eng.

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Virus contamination of water is a threat to human health in many countries. Current solutions for inactivation of viruses mainly rely on environmentally burdensome chemical oxidation or energy-intensive ultraviolet irradiation, which may create toxic secondary products. Here, we show that renewable plant biomass-sourced colloidal lignin particles (CLPs) can be used as agglomeration agents to facilitate removal of viruses from water. We used dynamic light scattering (DLS), electrophoretic mobility shift assay (EMSA), atomic force microscopy and transmission electron microscopy (AFM, TEM), and UV spectrophotometry to quantify and visualize adherence of cowpea chlorotic mottle viruses (CCMVs) on CLPs. Our results show that CCMVs form agglomerated complexes with CLPs that, unlike pristine virus particles, can be easily removed from water either by filtration or centrifugation. Additionally, cationic particles formed by adsorption of quaternary amine-modified softwood kraft lignin on the CLPs were also evaluated to improve the binding interactions with these anionic viruses. We foresee that due to their moderate production cost, and high availability of lignin as a side-stream from biorefineries, CLPs could be an alternative water pretreatment material in a large variety of systems such as filters, packed columns, or flocculants.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Agglomeration of Viruses by Cationic Lignin Particles for Facilitated Water Purification

Rivière

Korpi

Sipponen

et al. 2020

ACS Sustainable Chem. Eng.

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“…As an initial approach to determining the effect of nfGNPs on HPV16 infection, the reported inhibitory effect of HPV16 L1 VLPs on the aggregation of nfGNPs was further tested with HPV16 L1/L2 VLPs and PsVs, which closely resemble the natural virion. Challenging nfGNPs (100 µL) with L1/L2 VLPs or PsVs (200–500 ng) in aggregation assays showed that the inhibition of nfGNP aggregation is not exclusive for L1 VLPs [ 6 ] but was also extensive to L1/L2 VLPs ( Figure 3 A). This was expected because the L1 protein that comprises most of the HPV capsid and L2 is minimally exposed on the virion surface [ 8 ].…”

Section: Resultsmentioning

confidence: 99%

“…Our group first reported the spontaneous interaction between HPV16 L1 virus-like particles (VLPs) and nfGNPs, which resulted in the stabilization of purified VLPs and the inhibition of the nfGNPs’ salt-induced aggregation. Ionic-competition and genetic data showed that the nature of an nfGNP–VLP interaction was mainly hydrophobic [ 6 ]. Furthermore, nfGNPs covered substantial areas on the VLPs’ surface, suggesting that nfGNPs may be used as a steric inhibitor of the HPV-16 virion interaction with the host cell surface receptor, thus blocking infection.…”

Section: Introductionmentioning

confidence: 99%

Non-Functionalized Gold Nanoparticles Inhibit Human Papillomavirus (HPV) Infection

Valencia-Reséndiz

Villegas

Bahena

et al. 2022

IJMS

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The spontaneous interaction between human papillomavirus type 16 (HPV16) L1 virus-like particles (VLPs) and non-functionalized gold nanoparticles (nfGNPs) interferes with the nfGNPs’ salt-induced aggregation, inhibiting the red–blue color shift in the presence of NaCl. Electron microscopy and competition studies showed that color-shift inhibition is a consequence of direct nfGNP–VLP interaction and, thus, may produce a negative impact on the virus entry cell process. Here, an in vitro infection system based on the HPV16 pseudovirus (PsV) was used to stimulate the natural infection process in vitro. PsVs carry a pseudogenome with a reporter gene, resulting in a fluorescent signal when PsVs infect a cell, allowing quantification of the viral infection process. Aggregation assays showed that nfGNP-treated PsVs also inhibit color shift in the presence of NaCl. High-resolution microscopy confirmed nfGNP–PsV complex formation. In addition, PsVs can interact with silver nanoparticles, suggesting a generalized interaction of metallic nanoparticles with HPV16 capsids. The treatment of PsVs with nfGNPs produced viral infection inhibition at a higher level than heparin, the canonical inhibitor of HPV infection. Thus, nfGNPs can efficiently interfere with the HPV16 cell entry process and may represent a potential active component in prophylactic formulations to reduce the risk of HPV infection.

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“…1 A. The gold nanoparticle (AuNP) is most widely utilized due to its unique optical properties [ 39 ]. Recently, Basso et al [ 33 ] used a hybrid nanomaterial formed by coupling magnetic nanoparticles γ-Fe 2 O 3 to aptamer-modified AuNPs on the surface to develop a new colorimetric aptasensor.…”

Section: Detection Mechanismsmentioning

confidence: 99%

Aptamer-based biosensors for virus protein detection

Lou¹,

Liu²,

Shi³

et al. 2022

TrAC Trends in Analytical Chemistry

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Effect of HPV16 L1 virus-like particles on the aggregation of non-functionalized gold nanoparticles

Cited by 17 publications

References 47 publications

Agglomeration of Viruses by Cationic Lignin Particles for Facilitated Water Purification

Agglomeration of Viruses by Cationic Lignin Particles for Facilitated Water Purification

Non-Functionalized Gold Nanoparticles Inhibit Human Papillomavirus (HPV) Infection

Aptamer-based biosensors for virus protein detection

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