Effect of human chorionic gonadotrophin on the detachment of human granulosa cells from extracellular matrix layered onto glass or plastic

Aston, Kayleigh; O'Sullivan, MP; Thomas, Eric J.; Richardson, M. C.

doi:10.1093/humrep/11.2.336

Cited by 6 publications

(2 citation statements)

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“…Human granulosa cells cultured on a thin layer of ECM are lost from culture in the absence of gonadotropin (36). These cells are released from culture via an active process suppressed by hCG (43). One of the effects of hCG during maternal recognition of pregnancy appears to be the inhibition of metalloproteinase expression.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Luteal “Rescue” on the Expression and Localization of Matrix Metalloproteinases and Their Tissue Inhibitors in the Human Corpus Luteum

Duncan

McNeilly²,

Illingworth

1998

The Journal of Clinical Endocrinology & Metabolism

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Luteolysis is associated with tissue remodeling probably involving the matrix metalloproteinases (MMPs) and their specific tissue inhibitors (TIMPs). This study investigated the expression and localization of the major MMPs and TIMPs in the human corpus luteum throughout the luteal phase and after luteal rescue with hCG. Corpora lutea (n = 9) were collected at hysterectomy and were dated by serial urinary LH estimation. In addition, corpora lutea (n = 3) were collected from women who had received daily doubling doses of hCG to mimic the hormonal changes of early pregnancy. MMP-1, MMP-2, MMP-9, TIMP-1, TIMP-2, and TIMP-3 were investigated by zymography, reverse zymography, Northern blotting, and in situ hybridization. There was no change in the expression of MMP-1, TIMP-1, and TIMP-2 throughout the luteal phase or after luteal rescue. Little TIMP-3 could be detected in the corpus luteum. MMP-9 activity peaked in the early and late luteal phase. The expression and activity of MMP-2 were maximal in the late luteal phase. Exposure to hCG during luteal rescue in vivo was associated with a reduction (P < 0.05) in the expression and activity of MMP-2. Messenger ribonucleic acids (mRNAs) for MMP-1, MMP-2, and TIMP-2 were localized to the connective tissue stroma and the thecal-lutein cells of the corpus luteum. In contrast, TIMP-1 mRNA was localized to the granulosa-lutein cells, and MMP-9 mRNA was expressed in scattered cells within the steroidogenic and nonsteroidogenic cell layers. In conclusion, during maternal recognition of pregnancy, hCG prevents the normal increase in MMP-2 in the late luteal phase. MMPs can function in an environment containing large amounts of TIMP-1, as they have a different cellular localization.

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Section: Discussionmentioning

confidence: 99%

The Effect of Luteal “Rescue” on the Expression and Localization of Matrix Metalloproteinases and Their Tissue Inhibitors in the Human Corpus Luteum

Duncan

McNeilly²,

Illingworth

1998

The Journal of Clinical Endocrinology & Metabolism

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“…Often, observational studies of their expression in tissue sections do not clearly reveal local regulation. Luteinised granulosa cells from assisted conception cycles can be used to model luteal steroidogenic cells [59]. VEGF and EG-VEGF expression in these cells is regulated by hCG and not by progesterone [55].…”

Section: Molecular Regulation Of Luteal Angiogenesis In the Nonconcepmentioning

confidence: 99%

Vascular morphogenesis in the primate ovary

Fraser

Duncan

2005

Angiogenesis

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Ovarian function is dependent on intense cyclical vascular morphogenesis and regression. The molecular and cellular pathways involved in the generation of new capillary networks in the ovary are now being elucidated. Focussing on the marmoset, the course of angiogenesis at different stages of follicular maturation and in the corpus luteum throughout the cycle and in early pregnancy have been quantified and major progress has been made in the evaluation of the role of vascular endothelial growth factor (VEGF). To study the physiological role of VEGF in follicular and luteal angiogenesis in detail, VEGF was inhibited during defined stages of the cycle in vivo. VEGF antagonist administered throughout the follicular phase of the cycle resulted in a marked decrease in endothelial cell proliferation in developing antral follicles, accompanied by a decline in granulosa cell proliferation, restriction of follicular growth and inhibition of ovulation. An outstanding feature in the ovary is the intense angiogenesis that occurs during the early luteal phase. VEGF inhibitors markedly suppressed this angiogenesis, resulting in a marked restriction in the development of the microvascular tree and suppression of plasma progesterone. These studies showed that VEGF is essential for normal follicular and luteal angiogenesis and function, and demonstrated how luteal angiogenesis in particular could serve as a sensitive bioassay for putative angiogenic antagonists. Antagonists of VEGF are potent tools for investigating the role of angiogenic factors within the ovary and may have applications to the treatment of reproductive disorders characterised by alterations in normal vascular structure or function.

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Extracellular matrix of the bovine ovarian membrana granulosa

Rodgers

2002

Molecular and Cellular Endocrinology

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Effect of human chorionic gonadotrophin on the detachment of human granulosa cells from extracellular matrix layered onto glass or plastic

Cited by 6 publications

References 0 publications

The Effect of Luteal “Rescue” on the Expression and Localization of Matrix Metalloproteinases and Their Tissue Inhibitors in the Human Corpus Luteum

The Effect of Luteal “Rescue” on the Expression and Localization of Matrix Metalloproteinases and Their Tissue Inhibitors in the Human Corpus Luteum

Vascular morphogenesis in the primate ovary

Extracellular matrix of the bovine ovarian membrana granulosa

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