Effect of Human Chorionic Gonadotropin on the Expression of Progesterone Receptors and Estrogen Receptors in Rabbit Ovarian Granulosa Cells and the Uterus

Iwai, Toshiko; Fujii, Shingo; Nanbu, Yoshihiko; Nonogaki, Hirofumi; Konishi, Ikuo; Mori, Takahide; Okamura, Hitoshi

doi:10.1210/endo-129-4-1840

Cited by 46 publications

(26 citation statements)

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“…By contrast, ER mRNA was found predominantly in granulosa cells but also in thecal cells at a low level. This distribution for ER mRNA is consistent with the previous immunohistochemical data showing that granulosa cells are the major site of ER in ovary (52,56).…”

Section: Isolation Of Mouse Efp and Comparison With Other Proteins Insupporting

confidence: 92%

Molecular Cloning, Structure, and Expression of Mouse Estrogen-responsive Finger Protein Efp

Orimo

Inoue

Ikeda

et al. 1995

Journal of Biological Chemistry

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We have previously identified a human estrogen-responsive gene, efp (estrogen-responsive finger protein), which encodes a putative transcription regulator (Inoue, S., Orimo, A., Hosoi, T., Kondo, S., Toyoshima, H., Kondo, T., Ikegami, A., Ouchi, Y., Orimo, H., and Muramatsu, M. (1993) Proc. Natl. Acad. Sci. U. S. A. 90, 11117-11121). Here, we report isolation of mouse Efp cDNA and its structure containing three cysteine-rich domains (RING finger and B1 and B2 boxes), a coiledcoil domain, and a C-terminal domain. High levels of Efp mRNA were detected in uterus, ovary, and placenta by RNase protection assay. By in situ hybridization histochemistry the transcripts of efp were also detected in uterus, mammary gland, ovary, and brain, and the colocalization of Efp and estrogen receptor mRNA was particularly demonstrated in these female organs. Moreover, the level of Efp mRNA in uterus and brain, which are known as target organs for estrogen, was up-regulated in vivo by 17␤-estradiol. Furthermore, both the Efp and estrogen receptor mRNA were stained in the brain vesicles of 11.5-day embryos by whole mount in situ hybridization. These findings raise the possibility that efp is an estrogen-responsive gene that mediates estrogen action in various target organs.

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Section: Isolation Of Mouse Efp and Comparison With Other Proteins Insupporting

confidence: 92%

Molecular Cloning, Structure, and Expression of Mouse Estrogen-responsive Finger Protein Efp

Orimo

Inoue

Ikeda

et al. 1995

Journal of Biological Chemistry

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“…However, the inhibitory effect of progesterone on PR expression was not observed in granulosa cells (Iwai et al 1991, Natraj & Richards 1993, Jo et al 2002. In the present study, total PR mRNA level in cumulus cells of COCs which were cultured with FSH and LH was maintained at a high level for up to 20 h of cultivation.…”

Section: Pr In Cumulus Cells During Meiotic Resumption Of Oocytescontrasting

confidence: 54%

Expression of two progesterone receptor isoforms in cumulus cells and their roles during meiotic resumption of porcine oocytes

Shimada¹,

Yamashita²,

Ito³

et al. 2004

Journal of Molecular Endocrinology

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The present study aimed to investigate progesterone receptor (PR) gene expression in cumulus cells and their roles during meiotic resumption of porcine oocytes. The amount of PR-A or PR-B mRNA was analyzed by RT-PCR using primer sets for the PR-B region or the PR-A/B common region. The level of PR-B mRNA in cumulus cells was up-regulated by FSH and LH during the first 8 h of cultivation but the level significantly decreased at 12 h. However, a high level of total PR mRNA was maintained up to a cultivation period of 20 h. The level of PR-B protein in cumulus cells reached its maximum at 4 to 12 h, whereas PR-A predominated in cumulus cells of cumulus-oocyte complexes (COCs) at 20 h. Accompanying the shift in expression of PR isoforms, progesterone production in cumulus cells was significantly increased, and both the proliferative activity of cumulus cells during a 10-to 20-h cultivation period and the level of connexin-43, a major component of the gap junction, in cumulus cells significantly decreased. When COCs were cultured with FSH and LH for 10 h and then further cultured with additional RU486, there was a significant suppression in the shift in PR isoforms and in progesterone production, a loss of proliferative activity, and a decrease in connexin-43 mRNA in cumulus cells. Moreover, treatment with RU486 after 10-h cultivation of COCs inhibited the meiotic resumption of oocytes and cumulus cell expansion. These results suggest that the induction of PR isoforms in cumulus cells and their binding to progesterone appear to impact on proliferation and differentiation in a time-dependent manner, and the shift from PR-B to PR-A may help mediate certain events.

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“…ER-oestrogen interactions occur in the CL of other species (Telleira et al 1998, Chiang et al 2000, and in the tammar, tESR1 in conjunction with ESR2 may similarly be involved in the steroid secretory function of the CL which is controlled by the LH surge as well as in mediating autocrine and paracrine intraovarian regulation as observed in the human (Misao et al 1999) and mice (Byers et al 1997). Both tESR1 and tESR2 were co-expressed in the granulosa and theca cells of follicles at different stages of development, as seen in the rabbit (Iwai et al 1991) and human (Tetsuka et al 1998) but not in mice and rats. In the rat, ESR1 is localised to theca cells and interstitial tissue while ESR2 is present in granulosa cells (Hurst et al 1995, Couse & Korach 1999, Sar & Welsch 1999, Chiang et al 2000.…”

Section: Ers In the Developing And Adult Ovarymentioning

confidence: 88%

Ontogeny of the oestrogen receptors ESR1 and ESR2 during gonadal development in the tammar wallaby, Macropus eugenii

Calatayud¹,

Pask²,

Shaw³

et al. 2010

Reproduction

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Oestrogen has wide ranging effects in development mediated mainly via the two oestrogen receptors, a (ESR1, also known as ERa) and b (ESR2, also known as ERb). Oestrogen is the key factor that directs the indifferent gonad to become an ovary in many non-mammalian vertebrates. Oestrogen is not required for early ovarian differentiation in mammals but can disrupt normal testicular development in eutherians. Surprisingly, exogenous oestrogen can cause sex reversal of an XY gonad in two marsupials, the North American opossum and the tammar wallaby. To understand the mechanism by which oestrogen induces sex reversal, we characterised the genes for ESR1 and ESR2 and examined their expression during gonadal differentiation in the tammar wallaby, Macropus eugenii. Both receptors were expressed in the somatic cells and germ cells of the indifferent gonad in both XX and XY foetuses throughout all stages of development, and persisted in these cells into adulthood. ERs were also present in many other tissues including kidney, pituitary and mammary gland. ER mRNA was not significantly altered by exogenous oestrogen in cultured XY gonads but the receptors translocated to the nucleus in its presence. These findings confirm that there is conserved expression of the ERs in the indifferent gonad despite the lack of available ligand during early gonadal development. The receptors can respond to exogenous estrogen at this early stage and are capable of transducing signals in the early mammalian gonad. However, the selective forces that maintained conserved ER expression in this tissue remain unknown.

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Effect of Human Chorionic Gonadotropin on the Expression of Progesterone Receptors and Estrogen Receptors in Rabbit Ovarian Granulosa Cells and the Uterus

Cited by 46 publications

References 0 publications

Molecular Cloning, Structure, and Expression of Mouse Estrogen-responsive Finger Protein Efp

Molecular Cloning, Structure, and Expression of Mouse Estrogen-responsive Finger Protein Efp

Expression of two progesterone receptor isoforms in cumulus cells and their roles during meiotic resumption of porcine oocytes

Ontogeny of the oestrogen receptors ESR1 and ESR2 during gonadal development in the tammar wallaby, Macropus eugenii

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