Effect of human platelet supernatant on proliferation and matrix synthesis of human articular chondrocytes in monolayer and three-dimensional alginate cultures

Gaissmaier, Christoph; Fritz, Jürgen; Krackhardt, T.; Flesch, Ingo; Aicher, Wilhelm K.; Ashammakhi, Nureddin

doi:10.1016/j.biomaterials.2004.06.031

Cited by 114 publications

(89 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Interestingly, PRP or platelet supernatant stimulates the proliferation of articular chondrocytes, but has no effect on chondrocyte differentiation or even leads to dedifferentiation towards a fibroid phenotype as shown in bovine, ovine and human chondrocytes. [32][33][34] In contrast, PRP also stimulated the proliferation of mesenchymal stem cells but did not induce stem cell differentiation as shown in the ovine model. 34 In human mesenchymal stem cells, buffered PRP enhanced stem cell proliferation and induced the differentiation along the osteogenic and chondrogenic lineage as shown by the induction of the transcription factors Runx2 and Sox9 in monolayer cultures.…”

Section: Discussionmentioning

confidence: 90%

Human platelet‐rich plasma stimulates migration and chondrogenic differentiation of human subchondral progenitor cells

Krüger

Hondke

Endres

et al. 2011

Journal Orthopaedic Research

175

124

View full text Add to dashboard Cite

In cartilage repair, platelet-rich plasma (PRP) is used in one-step approaches utilizing microfracture and matrix-induced chondrogenesis procedures, bone marrow-derived cell transplantation, or intra-articular injection. The aim of our study was to evaluate the effect of human PRP on the migration and chondrogenic differentiation of human subchondral progenitors. Human progenitors were derived from subchondral cortico-spongious bone (CSP), were analyzed for their migration capacity upon PRP treatment in 96-well chemotaxis assays and cultured in high-density pellet cultures under serum-free conditions in the presence of 5% PRP. Chemotaxis assays showed that 0.1-100% PRP significantly (p < 0.05) stimulate the migration of CSP compared to untreated controls. Histological staining of proteoglycan and immuno-staining of type II collagen indicated that progenitors stimulated with PRP show significantly increased cartilage matrix formation compared to untreated progenitors. Real-time gene expression analysis of typical chondrocyte marker genes as well as osteogenic and adipogenic markers like osteocalcin and fatty acid binding protein showed that PRP induces the chondrogenic differentiation sequence of human progenitors in high-density pellet cultures, while osteogenic or adipogenic differentiation was not evident. These results suggest that human PRP may enhance the migration and stimulate the chondrogenic differentiation of human subchondral progenitor cells known from microfracture. ß

show abstract

Section: Discussionmentioning

confidence: 90%

Human platelet‐rich plasma stimulates migration and chondrogenic differentiation of human subchondral progenitor cells

Krüger

Hondke

Endres

et al. 2011

Journal Orthopaedic Research

175

124

View full text Add to dashboard Cite

show abstract

“…A 14-day incubation period was selected to allow for sufficient redifferentiation. 33 The type of alginate used for alginate culture influences the redifferentiation and metabolism of dedifferentiated chondrocytes. 34 As sub-experiments on hydrogels were conducted separately, a direct comparison of the alginate and alginate/agarose was not possible.…”

Section: Human Chondrocyte Gene Expression In Vitromentioning

confidence: 99%

Quantitative analysis of gene expression in human articular chondrocytes assigned for autologous implantation

Barlič

Drobnič

Maličev

et al. 2008

Journal Orthopaedic Research

View full text Add to dashboard Cite

Autologous chondrocyte implantation (ACI) relies on the implantation of in vitro expanded cells. The aim was to study the dedifferentiation of human articular chondrocytes under different cultivating conditions [days 0-10 in the primary culture (P0); passages in a monolayer from P0 to P3; monolayer vs. alginate and monolayer vs. alginate/agarose hydrogels] using real-time PCR analysis. The relative gene expressions for collagen type I and II, aggrecan and versican were quantified and the corresponding differentiation indexes (Col2/Col1, Agr/Ver) were calculated. The values of both differentiation indexes decreased exponentially with time in the P0 monolayer culture, and continued with a significant decrease over the subsequent monolayer passages. On the contrary, the chondrocytes seeded in either of the hydrogels significantly increased the indexes compared to their parallel monolayer cultures. These results indicate that alginate and alginate/agarose hydrogels offer an appropriate environment for human articular chondrocytes to redifferentiate after being expanded in vitro. Therefore the three-dimensional (3D) hydrogel chondrocyte cultures present not only surgical, but also biological advantage over the classic suspension-periosteum chondrocyte implantation. ß

show abstract

“…The scaffold materials of tissue engineering play an important role in growing microenvironment of chondrocytes. Previous studies have demonstrated that three-dimension culture such as alginate culture could induce the production of cartilage-specific extracellular matrix components and promote chondrogenesis in embryonic precursor cells 8) . Alginates have been used in a variety of clinical applications, because they are tolerated well when placed in contact with the body and they have a similar structure to glycosaminoglycans (GAG), one important constituent of the extracellular matrix 9) .…”

Section: Differences In Viscoelastic Properties Of Chondrocytes On DImentioning

confidence: 99%

Investigation for Effects of Cyclical Dynamic Compression on Matrix Metabolite and Mechanical Properties of Chondrocytes Cultured in Alginate

Zhou

Liu

Yang

et al. 2016

J. Hard Tissue Biology.

View full text Add to dashboard Cite

Articular cartilage is suffered from high mechanical loads under normal physiological conditions and articular chondrocytes regulate the composition of peri-cellular matrix (PCM), in response to mechanical signals. This study aims to investigate influence of cyclical dynamic compression on matrix metabolite and mechanical properties of chondrocytes cultured in alginate. Two months old New Zealand white rabbits were sacrificed by air embolism. Chondrocytes were taken from knee joints under aseptic conditions and cultured in alginate. The suspension was gelled in a shape of disc in CaCl 2 solution and processed for intermittent compression by using Flexcell-5000 compression system kept under an uncompressed condition as a control. The gene regulation of aggrecan (Agg), collagen (Col-2), matrix metallo-proteinases 13 (MMP-13) and collagen X (Col-10) were determined by reverse transcription polymerase chain reaction (RT-PCR) at 7th, 14th and 21st day post loading. The alteration in mechanical properties of chondrocytes induced by compression was evaluated using micropipette aspiration technique combined with a half-space model. A significant up-regulation was observed in gene expression of Agg and Col-2 on 7th day or MMP-13 and Col-10 on 14th day respectively after loading of compression. In response to compression, chondrocytes exhibited viscoelastic solid creep behavior. Young's modulus almost approximated to control group (0.65±0.42kPa). However, viscoelastic properties decreased gradually along with intermittent compression. Viscoelastic properties in experimental 21st day, including equilibrium modulus (E ), the instantaneous modulus (E 0 ) and apparent viscosity (μ) were significantly lower than experimental 7th day and 14th day (P<0.001), no significant difference was found between experimental 7th day and 14th day (P >0.05). In conclusion, chondrocytes cultured in alginate are stable of mechanical properties and suitable to construct seed cell of tissue engineering, and can keep phenotype and mechanical properties.

show abstract

Effect of human platelet supernatant on proliferation and matrix synthesis of human articular chondrocytes in monolayer and three-dimensional alginate cultures

Cited by 114 publications

References 38 publications

Human platelet‐rich plasma stimulates migration and chondrogenic differentiation of human subchondral progenitor cells

Human platelet‐rich plasma stimulates migration and chondrogenic differentiation of human subchondral progenitor cells

Quantitative analysis of gene expression in human articular chondrocytes assigned for autologous implantation

Investigation for Effects of Cyclical Dynamic Compression on Matrix Metabolite and Mechanical Properties of Chondrocytes Cultured in Alginate

Contact Info

Product

Resources

About