Effect of humic acid on fermentation and ciliate protozoan population in rumen fluid of sheep <i>in vitro</i>

Váradyová, Zora; Kišidayová, Svetlana; Jalč, D.

doi:10.1002/jsfa.3675

Cited by 26 publications

(36 citation statements)

References 40 publications

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“…Though the lack of effects of HA on whole digestibility of nutrients was not expected. Váradyová et al (2009) reported similar nutrients digestibility in in vitro batch cultures supplemented Table 2; SEM -means standard error;…”

Section: Discussionmentioning

confidence: 73%

Impact of humic acid as an organic additive on ruminal fermentation constituents, blood parameters and milk production in goats and their kids growth rate

El-Zaiat¹,

Morsy²,

Elwakeel³

et al. 2018

J. Anim. Feed Sci.

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Section: Discussionmentioning

confidence: 73%

Impact of humic acid as an organic additive on ruminal fermentation constituents, blood parameters and milk production in goats and their kids growth rate

El-Zaiat¹,

Morsy²,

Elwakeel³

et al. 2018

J. Anim. Feed Sci.

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“…Zależą one od zbilansowania dawki pokarmowej, a także od wzbogacenia jej w naturalne dodatki paszowe, takie jak: probiotyki, prebiotyki oraz związki humusowe (5,11). W nielicznych badaniach dotyczących wpływu kwasów huminowych na procesy trawienne w żwaczu stwierdzono zmniejszenie liczebności pierwotniaków i wzrost syntezy białka bakteryjnego oraz zmniejszenie stężenia lotnych kwasów tłuszczowych (3,11). Wykorzystanie składników odżywczych przez mikroorganizmy żwacza zależy od aktywności ich enzymów, dlatego poszukuje się takich dodatków paszowych, które wpłyną na procesy trawienne w żwaczu, a w konsekwencji polepszą jakość produktów końcowych -mięsa i mleka.…”

Section: Praca Oryginalnaunclassified

Influence of humic acid supplemented to sheep diets on rumen enzymatic activity

Majewska¹,

Miltko²,

Skomiał³

et al. 2017

Medycyna Weterynaryjna

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The objective of the present study was to investigate how different levels of natural humic acid in the diet affect the hydrolases activity in the rumen of sheep. The experiment was carried out on three rumen-fistulated adult sheep. The control animals were fed a diet (K) containing 59.7% meadow hay, 28.4% crushed barley, 9.8% soybean oil meal and 2.1% vitamin mineral mixture. The experimental diets were composed of the control ration supplemented with humic acid (HUMAC natur AFM® containing 65% humic acid in dry matter) and supplied at doses of 10 g · d–1 (diet H10) or 20 g · d–1 (diet H20). Samples of rumen digesta were collected just before the morning feeding, as well as 2, 4, and 8 h afterwards. In the present study, the cellulolytic activity in the rumen digesta was higher (P < 0.01) in sheep fed the K diet than it was in animals fed the H20 ration 2 h after feeding. However, the effect of humic acid on the cellulose degradation rate depended on the content of humic acid in the diet. The cellulolytic activity was increased 2 h after feeding in sheep fed the H10 diet as compared with animals fed the H20 ration (P < 0.05). Sampling time had significant influence on the hydrolases activity in the rumen of sheep. In sheep fed the H10 ration, the cellulolytic activity was higher 8 h after feeding than it was before feeding and 4 h after feeding (P < 0.05). In the H10 and H20 groups, the xylnalolytic activity was higher before feeding, but lower than it was in the control group K. The degradation rates of xylan were decreased 2 and 4 h after feeding in sheep fed the H10 and H20 rations and then increased 8 h after feeding (P < 0.05). In sheep fed the H20 diet, the amylolitic activity 8 h after feeding was higher than it was 2 h after feeding (P < 0.05). The effect of humic acid on the hydrolases activity in the rumen of sheep is not uniform and depends on the quantity of humic acid in the diet and on sampling time. Humic acid present in commercial HUMAC natur AFM® product enhances the initial rate of cellulose degradation in the rumen at a dose of 10 g · d-1. Thus, it may suggest that humic acid stimulates the growth of fibrolytic microorganisms in the rumen. On the other hand, the present results suggest that humic acid may reduce the rate of carbohydrate degradation in the rumen, since a higher hydrolases activity was observed before morning feeding and 8 h afterwards....

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“…Sığırlarda yapı-lan çalışmalarda 15,16,26 mevcut çalışmamızı destekler şekilde humik asitlerin rumen NH 3 -N'nu değiştirmediği bildirilirken, başka bir çalışmada 25 ise humik asitlerin lif içeriği ve enerji içeriği yüksek rasyonlarla beslenen koyunlarda NH 3 -N konsantrasyonunu azalttığını bildirilmişlerdir. Araş-tırıcılar bu durumu rumende bulunan hazır enerji kaynağı nedeniyle amino asitlerin mikroorganizmalarca enerji kaynağı olarak kullanılmayıp daha çok mikrobiyal protein sentezinde kullanılması böylece rumen NH 3 -N'nun azalmasıyla açıklamışlardır.…”

Section: Tartişma Ve Sonuçunclassified

“…Varadyova ve ark. 25 ise yüksek dozda humik asit ilave ettikleri in vitro çalışmada humik asitlerin rumende propiyonat düzeyini azalttığını tespit etmişlerdir. Ayrıca bütirat düzeyinde artış sağladığını ve asetat düzeyinde ise herhangi bir değişiklik meydana getirmediğini bildirilmişlerdir 25 .…”

Section: Tartişma Ve Sonuçunclassified

“…25 ise yüksek dozda humik asit ilave ettikleri in vitro çalışmada humik asitlerin rumende propiyonat düzeyini azalttığını tespit etmişlerdir. Ayrıca bütirat düzeyinde artış sağladığını ve asetat düzeyinde ise herhangi bir değişiklik meydana getirmediğini bildirilmişlerdir 25 . Aynı araştırıcılar Rusitec tekniğinde ise humik asitlerin lif içeriği ve enerji içeriği yüksek rasyonlarla beslenen koyunlarda TUYA üretimini azalttığını tespit etmiş-lerdir 25 .…”

Section: Tartişma Ve Sonuçunclassified

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The Effect of Humic Acid on Rumen Parameters, Protozoa Count and Blood Parameters in Sheep

Tunç¹,

Yörük²

2009

Kafkas Univ Vet Fak Derg

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ÖzetFarklı seviyelerdeki humik asit bileşiklerinin rumen fermantasyonu parametreleri, rumen protozoon sayısı ve kan parametreleri üzerine etkilerini incelemek amacıyla yürütülen araştırmada hayvan materyali olarak, rumen kanülü takılmış 4 adet 1.5 yaşlı morkaraman koçu kullanıldı. Araştırmada kaba/konsantre yem oranı 70/30 olarak ayarlandı. Humik asit preparatı Bovifarm® konsantre yeme 0, 0.1, 0.2 ve 0.4 olacak şekilde katıldı. Araştırma 4x4 Latin Kare deneme düzenine göre yürütüldü. Her bir dönem 14 günü adaptasyon, 6 günü örnekleme olmak üzere 20 gün sürdürüldü. Araştırma sonunda humik asit ilavesinin rumen sıvısı pH'sını, NH 3 -N düzeyini önemli derecede değiştirmediği (P>0.05), rumen sıvısı TUYA (Toplam Uçucu Yağ Asiti), TUYA içinde asetik asit, propiyonik asit, bütürik asit ve asetik asit/ propiyonik asit oranlarının humik asitlerden ve seviyelerinden önemli derecede etkilenmediği saptandı. Rumen protozoonlarının sayıları bakımından kontrol grubu ile humik asit içeren gruplar arasında görülen farklılıkların istatistiksel olarak önemli olmadığı tespit edildi (P>0.05). Kan örneklerinde yapılan analizler sonucunda kontrole göre humik asitlerin ve dozlarının serum total protein, albumin, trigliserit, kalsiyum, fosfor ve VLDL (çok düşük yoğunluklu lipoprotein) üzerine etkisinin önemli olmadığı ancak humik asitlerin tüm düzeylerinin kan serumu kolesterol ve LDL (düşük yoğunluklu lipoprotein) düzeyini düşürdüğü (P<0.05), HDL (yüksek yoğunluklu lipoprotein) düzeyini ise özellikle %0.2 ve 0.4 humik asit içeren gruplarda önemli (P<0.05) derecede artırdığı tespit edildi. Anahtar sözcükler: Humik asit, Kan parametreleri, Protozoon sayısı, Rumen fermantasyonu The Effect of Humic Acid on Rumen Parameters, Protozoa Count and Blood Parameters in Sheep SummaryThis study was conducted to investigate the effects of different levels of humic acids on ruminal fermentation, protozoa count and some blood parameters in sheep. Four ruminally cannulated morkaraman rams, 1.5 years old, were used in 4X4 Latin Square Desing with 14 days adaptation and 6 days sampling periods on the way 20 days total. The animals were offered 70% forage and 30% concentrate supplemented with %0, 0.1, 0.2 and 0.4 humate (Bovifarm®). Humic acid supplementation didn't effect the ruminal pH and ruminal ammonia-N (P>0.05). Neither TVFA (Total Volatile Fatty Acid) concentration nor the proportion of acetic acid, propionic acid, butyric acid and acetic/propionic acid ratio in TVFA were affected by supplements. No differences were found among groups for protozoa counts (P>0.05). In blood samples, there were no significant differences among levels for serum total protein, albumine, trigliseride, calcium, phosphorus and VLDL (Very low-density lipoprotein). However, all humic acids levels decreased (P<0.05) the serum cholesterol and LDL (Low-densite lipoprotein); but increased (P<0.05) the HDL (High-density lipoprotein) levels in groups supplemented with %0.2 and 0.4 humic acids, significantly.

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Effect of humic acid on fermentation and ciliate protozoan population in rumen fluid of sheep in vitro

Cited by 26 publications

References 40 publications

Impact of humic acid as an organic additive on ruminal fermentation constituents, blood parameters and milk production in goats and their kids growth rate

Impact of humic acid as an organic additive on ruminal fermentation constituents, blood parameters and milk production in goats and their kids growth rate

Influence of humic acid supplemented to sheep diets on rumen enzymatic activity

The Effect of Humic Acid on Rumen Parameters, Protozoa Count and Blood Parameters in Sheep

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