Effect of hyaluronic acid in bone formation and its applications in dentistry

Zhao, Ning; Wang, Xin; Qin, Lei; Zhai, Min; Yuan, Jing; Chen, Ji Hua; Li, Dehua

doi:10.1002/jbm.a.35681

Cited by 64 publications

(60 citation statements)

References 132 publications

(387 reference statements)

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“…Low MW HA was reported to increase cell proliferation in studies with rat and mouse mesenchymal stem cells, rabbit bone marrow‐derived stem cells as well as in an organotypic keratinocyte‐fibroblast co‐culture model . In contrast, the reported effects of high MW HA on cellular proliferation are controversial . A significant stimulatory as well as an inhibitory effect has been seen dependent on the cellular context, HA concentrations, and method of delivery of HA to the cell culture.…”

Section: Discussionmentioning

confidence: 99%

“…A number of studies describe the use of exogenous HA in non‐surgical and surgical periodontal therapy with generally beneficial but moderate effects on surrogate outcome variables of periodontal inflammation, ie, bleeding on probing and residual pocket depth . However, only few studies exist on the use of HA in reconstructive periodontal surgery .…”

Section: Introductionmentioning

confidence: 99%

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Activity of two hyaluronan preparations on primary human oral fibroblasts

Asparuhova

Kiryak

Eliezer

et al. 2018

J of Periodontal Research

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Background and Objective The potential benefit of using hyaluronan ( HA ) in reconstructive periodontal surgery is still a matter of debate. The aim of the present study was to evaluate the effects of two HA formulations on human oral fibroblasts involved in soft tissue wound healing/regeneration. Material and Methods Metabolic, proliferative and migratory abilities of primary human palatal and gingival fibroblasts were examined upon HA treatment. To uncover the mechanisms whereby HA influences cellular behavior, wound healing‐related gene expression and activation of signaling kinases were analyzed by qRT ‐ PCR and immunoblotting, respectively. Results The investigated HA formulations maintained the viability of oral fibroblasts and increased their proliferative and migratory abilities. They enhanced expression of genes encoding type III collagen and transforming growth factor‐β3, characteristic of scarless wound healing. The HA s upregulated the expression of genes encoding pro‐proliferative, pro‐migratory, and pro‐inflammatory factors, with only a moderate effect on the latter in gingival fibroblasts. In palatal but not gingival fibroblasts, an indirect effect of HA on the expression of matrix metalloproteinases 2 and 3 was detected, potentially exerted through induction of pro‐inflammatory cytokines. Finally, our data pointed on Akt, Erk1/2 and p38 as the signaling molecules whereby the HA s exert their effects on oral fibroblasts. Conclusion Both investigated HA formulations are biocompatible and enhance the proliferative, migratory and wound healing properties of cell types involved in soft tissue wound healing following regenerative periodontal surgery. Our data further suggest that in gingival tissues, the HA s are not likely to impair the healing process by prolonging inflammation or causing excessive MMP expression at the repair site.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Activity of two hyaluronan preparations on primary human oral fibroblasts

Asparuhova

Kiryak

Eliezer

et al. 2018

J of Periodontal Research

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“…HA has been utilized as a biomaterial in many medical applications due to its high biocompatibility with host tissues and its ability to act as a dermal augmentation filler, an adhesion barrier or a drug delivery carrier [16,47,48,49]. Its cross-linked feature has been shown to improve the overall mechanical properties of the scaffold material, further stimulating osteogenesis both in vitro and in vivo [21,22,23,24].…”

Section: Discussionmentioning

confidence: 99%

Hyaluronic Acid Gel-Based Scaffolds as Potential Carrier for Growth Factors: An In Vitro Bioassay on Its Osteogenic Potential

Fujioka‐Kobayashi

Benoit

Kobayashi

et al. 2016

JCM

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Hyaluronic acid (HA) has been utilized for a variety of regenerative medical procedures due to its widespread presence in connective tissue and perceived biocompatibility. The aim of the present study was to investigate HA in combination with recombinant human bone morphogenetic protein 9 (rhBMP9), one of the most osteogenic growth factors of the BMP family. HA was first combined with rhBMP9 and assessed for the adsorption and release of rhBMP9 over 10 days by ELISA. Thereafter, ST2 pre-osteoblasts were investigated by comparing (1) control tissue culture plastic, (2) HA alone, and (3) HA with rhBMP9 (100 ng/mL). Cellular proliferation was investigated by a MTS assay at one, three and five days and osteoblast differentiation was investigated by alkaline phosphatase (ALP) activity at seven days, alizarin red staining at 14 days and real-time PCR for osteoblast differentiation markers. The results demonstrated that rhBMP9 adsorbed within HA scaffolds and was released over a 10-day period in a controlled manner. While HA and rhBMP9 had little effect on cell proliferation, a marked and pronounced effect was observed for cell differentiation. rhBMP9 significantly induced ALP activity, mRNA levels of collagen1α2, and ALP and osteocalcin (OCN) at three or 14 days. HA also demonstrated some ability to induce osteoblast differentiation by increasing mRNA levels of OCN and increasing alizarin red staining at 14 days. In conclusion, the results from the present study demonstrate that (1) HA may serve as a potential carrier for various growth factors, and (2) rhBMP9 is a potent and promising inducer of osteoblast differentiation. Future animal studies are now necessary to investigate this combination approach in vivo.

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“…In noninfectious bone defects, such as bone fractures, the healing process mainly relies on osteoblast differentiation and bone regeneration . The difference between the management of infectious and noninfectious bone defects is the control of infection . Therefore, the local dosage of preparations that suppress inflammation and enhance osteogenesis may benefit the repair of AP with large sized bone defects.…”

Section: Introductionmentioning

confidence: 99%

Suppressing inflammation and enhancing osteogenesis using novel CS‐EC@Ca microcapsules

Han

Wang

et al. 2018

J Biomedical Materials Res

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The aim of this study was to investigate the suppression of inflammation and enhancement of osteogenesis using chitosan‐coated calcium hydroxide‐loaded microcapsules (CS‐EC@Ca microcapsules) in vivo. Circular defects were created in the mandibular bones of rabbits and filled with Ca(OH)2, Bio‐oss, or CS‐EC@Ca microcapsules, and rabbits without drug implantation served as the controls. Lipopolysaccharides were injected in situ daily in all groups for 7 days. Mandibular bones were investigated at 4 and 12 weeks after surgery using micro‐CT, histological observations, and real‐time PCR analysis. At the postoperation, there was more substantial nascent bone in the microcapsule and Bio‐oss groups than in the control group. The recovery of the rabbits in the Ca(OH)2 group was slower than the control group, as determined using micro‐CT and histological staining. Osteocalcin and collagen type I production was not significantly different between the microcapsule and Bio‐oss groups (p > 0.05), but the expression levels of the two molecules were significantly increased compared to the control and Ca(OH)2 groups at postoperation (p < 0.05). The mRNA transcript levels of inflammatory factors in the microcapsule group had the most reduced expression of IL‐6 and TNF‐α (p < 0.05). The microcapsules significantly reduced inflammation and promoted osteogenesis in this rabbit model of inflammatory bone destruction. Our findings indicate that CS‐EC@Ca microcapsules hold potential for use in apical periodontitis treatment. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 3222–3230, 2018.

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Effect of hyaluronic acid in bone formation and its applications in dentistry

Cited by 64 publications

References 132 publications

Activity of two hyaluronan preparations on primary human oral fibroblasts

Activity of two hyaluronan preparations on primary human oral fibroblasts

Hyaluronic Acid Gel-Based Scaffolds as Potential Carrier for Growth Factors: An In Vitro Bioassay on Its Osteogenic Potential

Suppressing inflammation and enhancing osteogenesis using novel CS‐EC@Ca microcapsules

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