Effect of Hydrofluoric Acid Etching Time on Titanium Topography, Chemistry, Wettability, and Cell Adhesion

Zahran, R.; Leal, Juan Ignacio Rosales; Valverde, M.; Vílchez, Miguel Ángel Cabrerizo

doi:10.1371/journal.pone.0165296

Cited by 60 publications

(47 citation statements)

References 34 publications

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“…Mechanical anchorage to surface topographical cues, surface energy and wettability are known as factors influencing cell adhesion. In the present study, our results clearly revealed that the hydrophobic disks (D56 disks) had a significantly reduced number of adherent cells compared with the hydrophilic disks (D0 disks), which was consistent with some previous studies 20, [24][25][26] . These results illustrate one of the positive characteristics of hydrophilic surfaces and suggest that the positive effects of hydrophilic surfaces shown in previous studies can be attributed to enhanced cell adhesion capacity and the resulting cellular interactions.…”

Section: Discussionsupporting

confidence: 93%

Influence of the wettability of different titanium surface topographies on initial cellular behavior

Nishimura

Ogino

Ayukawa

et al. 2018

Dental Materials Journal

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This study examined the influence of the time-dependent wettability of different surface topographies on initial cellular behavior. Titanium disks with smooth topography (SM) and three kinds of rough topography (sandblasted (SA), microtopography (M) and nanotopography (N)) were prepared. Time-dependent changes in surface wettability were observed in all surfaces as shown in previous studies. On SM surfaces, hydrophobic alteration influenced cell spreading and the activity of RhoA (a small GTPase protein of the Rho family), while no alterations were observed on rough surfaces except for the number of adherent cells. Serum adsorption could recover these functional deteriorations by hydrophobic alteration. These findings suggest that surface topography is a more potent regulator in initial cellular behaviors such as cell spreading and RhoA activation than surface wettability.

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Section: Discussionsupporting

confidence: 93%

Influence of the wettability of different titanium surface topographies on initial cellular behavior

Nishimura

Ogino

Ayukawa

et al. 2018

Dental Materials Journal

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“…For instance, acid etching method allows to obtain irregular and complex surface of Ti implants using strong acid solutions such as sulfuric acid (H 2 SO 4 ), hydrochloric acid (HCl), hydrofluoric acid (HF), nitric acid (HNO 3 ), and any combination of mentioned acid solutions [120]. Etching increases roughness and wettability of the implant [121] but exerts negative effect on its mechanical properties [120]. Surface modification technique combining acid-etching and sand blasting is defined as sand-blasted, large-grit, acid etched (SLA), which indicates application of an acid onto the blasted surface [120,122].…”

Section: Subtractive Modifications Of Biomaterials Surfacementioning

confidence: 99%

Osteoconductive and Osteoinductive Surface Modifications of Biomaterials for Bone Regeneration: A Concise Review

Kazimierczak

Przekora

2020

Coatings

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The main aim of bone tissue engineering is to fabricate highly biocompatible, osteoconductive and/or osteoinductive biomaterials for tissue regeneration. Bone implants should support bone growth at the implantation site via promotion of osteoblast adhesion, proliferation, and formation of bone extracellular matrix. Moreover, a very desired feature of biomaterials for clinical applications is their osteoinductivity, which means the ability of the material to induce osteogenic differentiation of mesenchymal stem cells toward bone-building cells (osteoblasts). Nevertheless, the development of completely biocompatible biomaterials with appropriate physicochemical and mechanical properties poses a great challenge for the researchers. Thus, the current trend in the engineering of biomaterials focuses on the surface modifications to improve biological properties of bone implants. This review presents the most recent findings concerning surface modifications of biomaterials to improve their osteoconductivity and osteoinductivity. The article describes two types of surface modifications: (1) Additive and (2) subtractive, indicating biological effects of the resultant surfaces in vitro and/or in vivo. The review article summarizes known additive modifications, such as plasma treatment, magnetron sputtering, and preparation of inorganic, organic, and composite coatings on the implants. It also presents some common subtractive processes applied for surface modifications of the biomaterials (i.e., acid etching, sand blasting, grit blasting, sand-blasted large-grit acid etched (SLA), anodizing, and laser methods). In summary, the article is an excellent compendium on the surface modifications and development of advanced osteoconductive and/or osteoinductive coatings on biomaterials for bone regeneration.

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“…As described in a previous study, a hydrophilic surface with high surface energy might affect cell attachment and cell proliferation. Certain researchers have suggested that osteoblast cells prefer a Ti surface with negative Ssk but low roughness . In this study, group F3 revealed negative shifts, meaning that they had more valleys or pits rather than a predominance of peaks, in comparison with group F2.…”

Section: Discussionmentioning

confidence: 50%

Effects of fluoride‐modified titanium surfaces with the similar roughness on RUNX2 gene expression of osteoblast‐like MG63 cells

Lee

Koak

Lim

et al. 2017

J Biomedical Materials Res

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Chemical modification of titanium surfaces by hydrofluoric acid (HF) is an effective method to improve bone responses on titanium implant surfaces. In this study, titanium disks were sandblasted with titanium oxide grits and modified with 0.2% and 0.4% of diluted HF under different exposure times of 40 and 60 s. Surface characteristics, such as surface chemical composition, surface topography, and surface wettability, were investigated. To examine MG63 osteoblast-like cell responses to fluoride-modified titanium surfaces with roughness similar to that of nonmodified surfaces, a cell proliferation assay was performed and gene expression levels of Runx2 were evaluated using real-time PCR. Fluoride-modified titanium surfaces revealed no significant roughness difference but with hydrophilic properties than control group SB. Moreover, the relative atomic concentration percentages of fluoride were 0.7, 1.5, and 2.8. As fluoride concentrations increased, surface wettability increased and cell proliferation began earlier. However, the gene expression levels of Runx2 increased earlier on surfaces with 1.5% fluoride, with significantly high surface skewness. There seems to be an optimal fluoride concentration percentage when gene expression levels of Runx2 were taken into consideration. In addition, surface parameters, such as surface wettability and surface skewness, seem to be important factors in the enhancement of osteoblast differentiation by HF. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 3102-3109, 2017.

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Effect of Hydrofluoric Acid Etching Time on Titanium Topography, Chemistry, Wettability, and Cell Adhesion

Cited by 60 publications

References 34 publications

Influence of the wettability of different titanium surface topographies on initial cellular behavior

Influence of the wettability of different titanium surface topographies on initial cellular behavior

Osteoconductive and Osteoinductive Surface Modifications of Biomaterials for Bone Regeneration: A Concise Review

Effects of fluoride‐modified titanium surfaces with the similar roughness on RUNX2 gene expression of osteoblast‐like MG63 cells

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