Effect of hypoxia on hemoglobin isomorph abundances in rainbow trout, Salmo gairdneri

Abstract

To further assess the hypothesis that differential hemoglobin isomorph formation may be involved in the respiratory response of rainbow trout (Salmo gairdneri), yearling animals were first rendered acutely anemic by immersion in phenylhydrazine HCl. Erythropoiesis was then induced by gradual warming under normoxic and hypoxic conditions. While no significant differences in hemoglobin content were observed, trout exposed to hypoxia displayed transient, but significant differences in hematocrit. Significant variations in both the occurrence and abundance of specific isomorphs were also observed.

Hypoxia induces a complex response of globin expression in zebrafish(Danio rerio)

“…We cannot exclude that hypoxia, for example, induces the expression of embryonic Hbs. In fact, it has been observed by gel electrophoresis that hypoxia changes the pattern of Hb chains in trout (Marinsky et al, 1990). However, based on the observation of Ton et al (Ton et al, 2003) of a downregulation of embryonic Hbs in embryos, we consider this explanation less likely.…”

Cardiac remodelling in rainbow troutOncorhynchus mykissWalbaum in response to phenylhydrazine-induced anaemia

“…Significant differences among data for normocythemic, anaemic and severely anaemic statesare indicated by different letters, as determined by repeated-measures ANOVA and Tukey-Kramer multiple-range test (P<0.05). and Davis, 1970;Smith et al, 1971;Chudzik and Houston, 1983;Houston and Keen, 1984;Murad et al, 1990;Marinsky et al, 1990;Gilmour and Perry, 1996;McClelland et al, 2005), other than those directly related to the anaemia itself. A single study on anaemic rats reported that the observed increase in coronary flow during cardiac contractions following PHZ treatment was prevented by an antioxidant, as well as reducing focal lesions in the muscle and the degree of hypertrophy (Meerson and Evsevieva, 1985).…”

“…Phenylhydrazine hydrochloride has been widely used to study experimental anaemia in a range of animals (Itano et al, 1976), including fish (Cameron and Wohlschlag, 1969;Cameron and Davis, 1970;Jones, 1971;Smith and Jones, 1982;Chudzik and Houston, 1983;Houston and Keen, 1984;Murad et al, 1990;Marinsky et al, 1990;Gilmour and Perry, 1996;McClelland et al, 2005 As there was some variability in the time for the onset of anaemia among fishes, sampling interval varied for each fish such that the anaemic and severe anaemic data were generally collected 24-72·h and 72-96·h, respectively, after injection. The maximal period for data collection postinjection for any fish was 4 days.…”

The amphibious fishKryptolebias marmoratususes alternate strategies to maintain oxygen delivery during aquatic hypoxia and air exposure

“…The Journal of Experimental Biology (2014) (Grigg, 1969;Marinsky et al, 1990;Jensen, 1991;Val, 2000;Weber, 2000;Rutjes et al, 2007). Most commonly, air-breathing fishes increase organic phosphate concentrations relative to [Hb] during behavioral or ontogenetic shifts to air-breathing in order to facilitate O 2 unloading at the tissues (Babiker, 1984;Graham, 1997;Val, 2000).…”

“…Thus, Hb-O 2 affinity may be maintained in K. marmoratus under a variety of environmental conditions, regardless of widely variable blood CO 2 levels. Like Hb in mangrove rivulus, protein function in other animals is often highly conserved during acclimation to changing environmental conditions (Somero, 1995 (8) (Grigg, 1969;Marinsky et al, 1990;Jensen, 1991;Val, 2000;Weber, 2000;Rutjes et al, 2007). Most commonly, air-breathing fishes increase organic phosphate concentrations relative to [Hb] during behavioral or ontogenetic shifts to air-breathing in order to facilitate O 2 unloading at the tissues (Babiker, 1984;Graham, 1997;Val, 2000).…”