Effect of Hypoxia on the Differentiation and the Self-Renewal of Metanephrogenic Mesenchymal Stem Cells

Liu, Shaopeng; Song, Nana; He, Jiekun; Lei, Yu; Guo, Jia; Jiao, Xiaoyan; Ding, Xiaoqiang; Teng, Jie

doi:10.1155/2017/7168687

Cited by 9 publications

(5 citation statements)

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“…Previous study has shown that the hypoxia and HGF treatment enhance the differentiation of umbilical cord blood-derived stromal cells into IPCs (Sun et al, 2015 ). Additionally, hypoxic conditions promoted the differentiation and depressed the self-renewal of metanephrogenic mesenchymal stem cells (Liu et al, 2017 ). In contrast, there is evidence that oxygen influences the different cell subsets during pancreatic development.…”

Section: Discussionmentioning

confidence: 99%

Establishment of Insulin-Producing Cells From Human Embryonic Stem Cells Underhypoxic Condition for Cell Based Therapy

Rattananinsruang

Dechsukhum

Leeanansaksiri

2018

Front. Cell Dev. Biol.

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Diabetes mellitus (DM) is a group of diseases characterized by abnormally high levels of glucose in the blood stream. In developing a potential therapy for diabetic patients, pancreatic cells transplantation has drawn great attention. However, the hinder of cell transplantation for diabetes treatment is insufficient sources of insulin-producing cells. Therefore, new cell based therapy need to be developed. In this regard, human embryonic stem cells (hESCs) may serve as good candidates for this based on their capability of differentiation into various cell types. In this study, we designed a new differentiation protocol that can generate hESC-derived insulin-producing cells (hES-DIPCs) in a hypoxic condition. We also emphasized on the induction of definitive endoderm during embryoid bodies (EBs) formation. After induction of hESCs differentiation into insulin-producing cells (IPCs), the cells obtained from the cultures exhibited pancreas-related genes such as Pdx1, Ngn3, Nkx6.1, GLUT2, and insulin. These cells also showed positive for DTZ-stained cellular clusters and contained ability of insulin secretion in a glucose-dependent manner. After achievement to generated functional hES-DIPCs in vitro, some of the hES-DIPCs were then encapsulated named encapsulated hES-DIPCs. The data showed that the encapsulated cells could possess the function of insulin secretion in a time-dependent manner. The hES-DIPCs and encapsulated hES-DIPCs were then separately transplanted into STZ-induced diabetic mice. The findings showed the significant blood glucose levels regulation capacity and declination of IL-1β concentration in all transplanted mice. These results indicated that both hES-DIPCs and encapsulated hES-DIPCs contained the ability to sustain hyperglycemia condition as well as decrease inflammatory cytokine level in vivo. The findings of this study may apply for generation of a large number of hES-DIPCs in vitro. In addition, the implication of this work is therapeutic value in type I diabetes treatment in the future. The application for type II diabetes treatment remain to be investigated.

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Section: Discussionmentioning

confidence: 99%

Establishment of Insulin-Producing Cells From Human Embryonic Stem Cells Underhypoxic Condition for Cell Based Therapy

Rattananinsruang

Dechsukhum

Leeanansaksiri

2018

Front. Cell Dev. Biol.

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“…SC are multipotent cells, and depending on the stimuli and stress type, duration, and severity, they can follow myogenic, adipogenic, or osteogenic pathways ( Asakura et al., 2001 ; Shefer et al., 2004 ; Wang et al., 2015a ; Liu et al., 2017 ). As WB is characterized by infiltration of fat cells, we sought to evaluate here the potential activation of adipogenic program in SC under hypoxic conditions.…”

Section: Discussionmentioning

confidence: 99%

“…Muscle satellite cells ( SC ), stem cells located between the basement membrane and sarcolemma of muscle fiber ( Moss and Leblond, 1971 ), are self-renewing mesenchymal cells that enable hypertrophy, maintenance, and repair of damaged skeletal muscle ( Geiger et al., 2018 ). It has been shown that SC are multipotent and can be induced by various extrinsic factors and environment to follow myogenic, adipogenic, or osteogenic pathways ( Asakura et al., 2001 ; Wang et al., 2015a ; Liu et al., 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Hypoxia further exacerbates woody breast myopathy in broilers via alteration of satellite cell fate

et al. 2021

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Woody breast ( WB ) condition has created a variety of challenges for the global poultry industry. To date, there are no effective treatments or preventative measures due to its unknown (undefined) etiology. Several potential mechanisms including oxidative stress, fiber-type switching, cellular damage, and altered intracellular calcium levels have been proposed to play a key role in the progression of the WB myopathy. In a previous study, we have shown that WB is associated with hypoxia-like status and dysregulated oxygen homeostasis. As satellite cells ( SC ) play a pivotal role in muscle fiber repair and remodeling under stress conditions, we undertook the present study to determine satellite cell fate in WB-affected birds when reared in either normoxic or hypoxic conditions. Modern random bred broilers from 2015 (n = 200) were wing banded and reared under standard brooding practices for the first 2 wk post-hatch. At 15 d, chicks were divided in 2 body weight-matched groups and reared to 6 wk in either control local altitude or hypobaric chambers with simulated altitude of 6,000 ft. Birds were provided ad libitum access to water and feed, according to the Cobb recommendations. At 6 wk of age, birds were processed and scored for WB, and breast samples were collected from WB-affected and unaffected birds for molecular analyses (n = 10/group). SCs were isolated from normal breast muscle, cultured in vitro, and exposed to normoxia or hypoxia for 2 h. The expression of target genes was determined by qPCR using 2 −∆∆Ct method. Protein distribution and expression were determined by immunofluorescence staining and immunoblot, respectively. Data were analyzed by the Student's t test with significance set at P < 0.05. Multiple satellite cell markers, myogenic factor ( Myf )-5 and paired box ( PAX )-7 were significantly decreased at the mRNA and protein levels in the breast muscle from WB-affected birds compared to their unaffected counterparts. Lipogenic-and adipogenic-associated factors (acetyl-CoA carboxylase, ACCα ; fatty acid synthase, FASN , malic enzyme, ME ; and ATP citrate lyase, ACLY ) were activated in WB-affected birds. These data were supported by an in vitro study where hypoxia decreased the expression of Myf5 and Pax7, and increased that of ACCα, FASN, ME, and ACLY. Together, these data indicate that under hypoxic condition, SC change fate by switching from a myogenic to an adipogenic program, which explains at least partly, the etiology of the WB myopathy.

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“…It is also suggested that mGSCs preferentially reside in hypoxic regions of the ST, which facilitates metabolic pathways that are integral for the ongoing regenerative capacity of the cells [23]. Though bene cial effects of hypoxic culture on proliferation and differentiation potentials of speci c cell lines have been suggested by several groups [12,27,19], other studies showed contrasting results with negative or no effects of hypoxia on stem cell proliferation and differentiation [2,21]. These discrepancies in the results may be due to the variation in the O 2 level, the duration of trial, range of biological characteristics compared with type and number of stem cell population, and the variation in the sensitivity of system that was used to regulate the O 2 level.…”

Section: Discussionmentioning

confidence: 99%

Low oxygen tension potentiates proliferation and stemness but not multilineage differentiation of caprine male germline stem cells

et al. 2021

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The milieu of testicular germline stem cells (mGSCs) is characterized as low oxygen (O 2 ) environment, whereas, there in-vitro expansion is typically performed under normoxia (20-21% O 2 ). Here, we evaluated and compared the culture and multilineage differentiation characteristics of enriched (through differential platting and percoll density centrifugation) caprine mGSCs (cmGSCs) under hypoxic (5% O 2 ) and normoxic (21% O 2 ) culture conditions. For this, in addition to growth characteristics and populationdoubling time (PDT); viability, proliferation, senescence, and expression of key-markers of adhesion (βintegrin and E-Cadherin) and stemness (OCT-4, THY-1 and UCHL-1) were evaluated and compared under normoxia and hypoxia. Moreover, the extent of multilineage differentiation (neurogenic, adipogenic, and chondrogenic differentiation) was assessed. The survival, viability and proliferation were signi cantly promoted and PDT was reduced (p < 0.05), thus yielding a higher number of viable cells with larger colonies under hypoxia. Furthermore, expression of stemness and adhesion markers was distinctly increased under lowered O 2 condition. Conversely, the presence of differentiated regions and expression of differentiation speci c key genes [C/EBPα (adipogenic), nestin and β-tubulin (neurogenic), and COL2A1 (chondrogenic)] were signi cantly (p < 0.05) reduced under hypoxic conditions. These data demonstrate that culturing cmGSCs under hypoxia augments the growth characteristics, and stemness but not the multilineage differentiation potential of cmGSCs as compared with normoxia. These data are important for the development of robust methodologies for ex-vivo expansion and lineage-committed differentiation of cmGSCs for clinical applications.

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Effect of Hypoxia on the Differentiation and the Self-Renewal of Metanephrogenic Mesenchymal Stem Cells

Cited by 9 publications

References 50 publications

Establishment of Insulin-Producing Cells From Human Embryonic Stem Cells Underhypoxic Condition for Cell Based Therapy

Establishment of Insulin-Producing Cells From Human Embryonic Stem Cells Underhypoxic Condition for Cell Based Therapy

Hypoxia further exacerbates woody breast myopathy in broilers via alteration of satellite cell fate

Low oxygen tension potentiates proliferation and stemness but not multilineage differentiation of caprine male germline stem cells

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