Effect of hypoxia on the proliferation of porcine bone marrow-derived mesenchymal stem cells and adipose-derived mesenchymal stem cells in 2- and 3-dimensional culture

Burian, Egon; Probst, Florian; Palla, Benjamin; Riedel, Christina; Saller, Maximilian Michael; Cornelsen, Matthias; König, Florian; Schieker, Matthias; Otto, Sven

doi:10.1016/j.jcms.2016.12.014

Cited by 20 publications

(14 citation statements)

References 39 publications

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“…Finally, in the present review, calcium deposits maintained the same values compared with the normoxia groups in four studies, after low oxygen availability between 1% to 5% PiO 2 was applied for 14–21 days [33,37,38,41]. In BMSCs, the most severe doses (<2%) showed decreased calcium deposits [27,29,40,41,46,49,51,52,76]. Previous researchers have suggested that there may exist a basal threshold of tissue oxygenation that regulates the deposition of minerals in the extracellular matrix [53].…”

Section: Discussionmentioning

confidence: 56%

“…Effects of hypoxia on osteogenic differentiation may be time-dependent: osteogenesis could be accelerated in the early period, but sustained long-term hypoxia could result in poor osteogenesis [27]. On the other hand, previous studies have reported that under low O 2 conditions, MSCs proliferate faster and for a longer period of time [29]. However, maintaining the exposure during cellular differentiation could maintain the undifferentiated characteristics of these cells [40].…”

Section: Discussionmentioning

confidence: 99%

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Can Hypoxic Conditioning Improve Bone Metabolism? A Systematic Review

Camacho-Cardeñosa

Timón

et al. 2019

IJERPH

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Among other functions, hypoxia-inducible factor plays a critical role in bone–vascular coupling and bone formation. Studies have suggested that hypoxic conditioning could be a potential nonpharmacological strategy for treating skeletal diseases. However, there is no clear consensus regarding the bone metabolism response to hypoxia. Therefore, this review aims to examine the impact of different modes of hypoxia conditioning on bone metabolism. The PubMed and Web of Science databases were searched for experimental studies written in English that investigated the effects of modification of ambient oxygen on bone remodelling parameters of healthy organisms. Thirty-nine studies analysed the effect of sustained or cyclic hypoxia exposure on genetic and protein expression and mineralisation capacity of different cell models; three studies carried out in animal models implemented sustained or cyclic hypoxia; ten studies examined the effect of sustained, intermittent or cyclic hypoxia on bone health and hormonal responses in humans. Different modes of hypoxic conditioning may have different impacts on bone metabolism both in vivo and in vitro. Additional research is necessary to establish the optimal cyclical dose of oxygen concentration and exposure time.

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Section: Discussionmentioning

confidence: 56%

Section: Discussionmentioning

confidence: 99%

Can Hypoxic Conditioning Improve Bone Metabolism? A Systematic Review

Camacho-Cardeñosa

Timón

et al. 2019

IJERPH

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“…However, a variety of studies describes a predominantly positive effect of hypoxia on proliferation and differentiation of MSCs and in particular ADSCs 33 . In a previous work, our study group demonstrated in 2D and 3D cell culture (3D cultivation on TCP-polyhydroxybuturate composite scaffolds) that proliferation capacity of both porcine BMSCs and ADSCs was higher under hypoxic conditions (2% oxygen) in relation to normoxic conditions (21% oxygen) 34 . With regard to osteogenic differentiation, BMMSCs showed highly decreased differentiation capacity under hypoxic conditions while ADSCs had a tendency towards increased osteogenic capacity 34 .…”

Section: Discussionmentioning

confidence: 80%

“…In a previous work, our study group demonstrated in 2D and 3D cell culture (3D cultivation on TCP-polyhydroxybuturate composite scaffolds) that proliferation capacity of both porcine BMSCs and ADSCs was higher under hypoxic conditions (2% oxygen) in relation to normoxic conditions (21% oxygen) 34 . With regard to osteogenic differentiation, BMMSCs showed highly decreased differentiation capacity under hypoxic conditions while ADSCs had a tendency towards increased osteogenic capacity 34 . These results are in line with those of a previous study dealing with hypoxic preconditioning of BMMSCs 35 .…”

Section: Discussionmentioning

confidence: 80%

Bone regeneration of minipig mandibular defect by adipose derived mesenchymal stem cells seeded tri-calcium phosphate- poly(D,L-lactide-co-glycolide) scaffolds

Probst

Fliefel

Burian

et al. 2020

Sci Rep

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Reconstruction of bone defects represents a serious issue for orthopaedic and maxillofacial surgeons, especially in extensive bone loss. Adipose-derived mesenchymal stem cells (ADScs) with tri-calcium phosphates (tcp) are widely used for bone regeneration facilitating the formation of bone extracellular matrix to promote reparative osteogenesis. The present study assessed the potential of cell-scaffold constructs for the regeneration of extensive mandibular bone defects in a minipig model. Sixteen skeletally mature miniature pigs were divided into two groups: Control group and scaffolds seeded with osteogenic differentiated pADSCs (n = 8/group). TCP-PLGA scaffolds with or without cells were integrated in the mandibular critical size defects and fixed by titanium osteosynthesis plates. After 12 weeks, ADSCs seeded scaffolds (n = 7) demonstrated significantly higher bone volume (34.8% ± 4.80%) than scaffolds implanted without cells (n = 6, 22.4% ± 9.85%) in the micro-CT (p < 0.05). Moreover, an increased amount of osteocalcin deposition was found in the test group in comparison to the control group (27.98 ± 2.81% vs 17.10 ± 3.57%, p < 0.001). In conclusion, ADSCs seeding on ceramic/polymer scaffolds improves bone regeneration in large mandibular defects. However, further improvement with regard to the osteogenic capacity is necessary to transfer this concept into clinical use. Maxillofacial bone defects, which occur due to trauma, craniofacial deformities, tumour or infection can lead to facial deformities and severe maxillofacial dysfunctions provoking a dramatic decrease in the quality of life of the patients 1,2. The reconstruction of large bone defects poses many challenges in oral and maxillofacial surgery. Although autologous bone grafts are considered the gold standard in bone defect repair, there are some concerns related to the limited supply and donor site morbidity 3. Several alternatives as allografts, xenografts or synthetic bone substitutes have been brought by researchers and clinicians to restore the function and architecture of the defective bone but still cannot solve the problem due to various limitations. The search of new treatment alternatives has emerged enormously in the past few

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“…This surgical choice is characterized by its simplicity, low associated complications, mechanical stability, and good vascularization. Moreover, fat grafts contain stem cells which can differentiate into different cell types and therefore can promote tissue healing, including bone tissue healing (Burian et al, ; Farre‐Guasch, Marti‐Page, Hernadez‐Alfaro, Klein‐Nulend, & Casals, ). Melville et al () and Rotaru et al () reported 23 and 10 cases of MRONJ effectively treated with BFPF, respectively.…”

Section: Discussionmentioning

confidence: 99%

Surgical treatment of medication‐related osteonecrosis of the upper jaw: Case series

et al. 2018

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Purpose The management of maxillary medication‐related osteonecrosis of the jaw (MRONJ) is challenging. Therefore, identifying the proper treatment is important. This study aimed to evaluate the surgical treatment of maxillary MRONJ using single‐layer closure with mucoperiosteal flap and double‐layer closure with buccal fat pad flap (BFPF) and mucoperiosteal flap and to find the outcomes after rehabilitation with obturators. Methods A retrospective analysis was conducted and included all surgically treated and followed‐up maxillary MRONJ cases in a single center. Demographics and clinical data, stage of MRONJ, surgical treatment, and treatment outcome were collected. Results Seventy‐nine lesions were included. Removal of necrotic bone was followed by coverage with mucoperiosteal flap in 60 lesions and BFPF in 14 lesions. Seven lesions (five primarily and two following unsuccessful treatment with BFPF) underwent necrectomy and were reconstructed with obturators. Complete mucosal healing was achieved in 76.7% of the lesions covered with mucoperiosteal flap. BFPF led to complete mucosal healing in 85.7% of the lesions. No complications were observed in the defects rehabilitated with obturators. Conclusion Removal of necrotic bone followed by closure with mucoperiosteal flap is reliable for MRONJ treatment. BFPF is effective for closure of MRONJ‐related oroantral communications (OACs).

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Effect of hypoxia on the proliferation of porcine bone marrow-derived mesenchymal stem cells and adipose-derived mesenchymal stem cells in 2- and 3-dimensional culture

Cited by 20 publications

References 39 publications

Can Hypoxic Conditioning Improve Bone Metabolism? A Systematic Review

Can Hypoxic Conditioning Improve Bone Metabolism? A Systematic Review

Bone regeneration of minipig mandibular defect by adipose derived mesenchymal stem cells seeded tri-calcium phosphate- poly(D,L-lactide-co-glycolide) scaffolds

Surgical treatment of medication‐related osteonecrosis of the upper jaw: Case series

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