Effect of <i>Bupleuri Radix</i> Extracts on the Toxicity of 5‐Fluorouracil in HepG2 Hepatoma Cells and Normal Human Lymphocytes

Kang, Su Jin; Lee, Young Joon; Kim, Byeong Mo; Kim, Yang Jee; Woo, Hae Dong; Jeon, Hye Kyoung; Chung, Hai Won

doi:10.1111/j.1742-7843.2008.00280.x

Cited by 43 publications

(24 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In hepatoma cells, 5-FU induced the up-regulation of Bax, p53, and p21 and triggered the collapse of the mitochondria membrane potential [28]. 5-FU also induced expression of the pro-apoptotic proteins (caspases-8, -9, -3, and Bax) in HeLa cervical carcinoma [29].…”

Section: Discussionmentioning

confidence: 93%

Mechanism of 5-Fluorouracil-Induced Apoptosis on Cultured Corneal Endothelial Cells

Hong¹,

Wu²,

Lai³

et al. 2014

OJApo

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 93%

Mechanism of 5-Fluorouracil-Induced Apoptosis on Cultured Corneal Endothelial Cells

Hong¹,

Wu²,

Lai³

et al. 2014

OJApo

View full text Add to dashboard Cite

show abstract

“…Jurkat T-cells were exposed to PEI or PAMAM polymers for 6 hours and then incubated with DiOC 6 (40 nM) for 30 minutes at 37°C. Cells were washed twice with PBS and analyzed by using flow cytometry (FACSCalibur; Becton-Dickinson) (Kang et al, 2008). All experiments were performed at least three times.…”

Section: Flow Cytometrymentioning

confidence: 99%

Comparative studies on the genotoxicity and cytotoxicity of polymeric gene carriers polyethylenimine (PEI) and polyamidoamine (PAMAM) dendrimer in Jurkat T-cells

Choi

Kang

Kim

et al. 2010

Drug and Chemical Toxicology

Self Cite

View full text Add to dashboard Cite

A safe alternative to the viral system used in gene therapy is a nonviral gene delivery system. Although polyethylenimine (PEI) and polyamidoamine (PAMAM) dendrimer are among the most promising gene-carrier candidates for efficient nonviral gene delivery, safety concerns regarding their toxicity remain. The aim of this study was to scrutinize the underlying mechanism of the cytotoxicity and genotoxicity of PEI (25 kDa) and PAMAM (G4). To our knowledge, this is the first study to explore the genotoxic effect of polymeric gene carriers. To evaluate cell death by PEI and PAMAM, we performed propidium-iodide staining and lactate-dehydrogenase release assays. The genotoxicity of the polymers was measured by comet assay and cytokinesis-block micronucleus assay. PEI- and PAMAM-treated groups induced both necrotic and apoptotic cell death. In the comet assay and micronuclei formation, significant increases in DNA damage were observed in both treatments. We conclude that PEI and PAMAM dendrimer can induce not only a relatively weak apoptotic and a strong necrotic effect, but also a moderate genotoxic effect.

show abstract

“…Chaihu, Danggui, and Shanzhi are the three identical herbs used in both TCHYS and LDSGT. Extracts from Chaihu (Bupleuri chinese) may be effective as a therapeutic agent to treat hepatomas only, because it increased mitochondria membrane potential collapse in HepG2 cells, but not in lymphocytes 18 . Danggui (Angelicae sinensis) has been reported to possess immunomodulatory functions, as the treatment of Danggui-containing TCM to cancer patients, who were receiving chemotherapy and/or radiotherapy, showed improvement in their immune functions 5 .…”

Section: Discussionmentioning

confidence: 99%

Two Chinese Herbal Regimens Safe for the Elderly on Inhibiting Liver and Bladder Tumor Cell Growth and Regulating Gene Expression

Chen¹,

Lü²,

Wang³

et al. 2011

International Journal of Gerontology

View full text Add to dashboard Cite

Background: Cancers have become one of the most lethal diseases in elders. In traditional Chinese medicine, Tan-Chih-Hsiao-Yao-San (TCHYS) and Long-Daan-Shiah-Gan-Tang (LDSGT) are used to treat cancers. However, the growth-inhibitory effects and gene expression profiles of these drugs on cancer cells are still unclear. Methods: This study assessed the effects of TCHYS and LDSGT on viability of liver and bladder tumor cells, and bladder TCCSUP cells were further subjected to profile gene expression patterns with microarray technology for identifying gene candidates that may be involved in the tumorigenesis. Results: The results revealed that both drugs significantly eliminated the growth of Chang liver and three hepatoma cells. On the contrary, the embryonic liver WRL68 cells showed less response to the treatments, whereas the control agent genistein had much higher inhibitory effect in WRL68 cells than in the other hepatoma cells. Both TCHYS and LDSGT, as well as cisplatin and paclitaxel, exhibited dose-dependent suppression on the viability of all bladder cancer cells. To characterize the possible regulation for such effects, the profiling of gene expression was performed with complementary DNA chips. When bladder transitional cell carcinoma (TCC) TCCSUP cells were treated with TCHYS, 29 upregulated and 28 downregulated genes were detected; whereas 54 genes were upregulated in the same cells treated with LDSGT. Moreover, the detected gene expression patterns were also confirmed by using the reverse transcription-polymerase chain reaction assay. Conclusion: This study initiates the evaluations of drug efficacies and gene expression profiles of traditional Chinese medicines, which may provide important information and identify useful biomarkers for treating cancers. Copyright (C) 2011, Taiwan Society of Geriatric Emergency & Critical Care Medicine. Published by Elsevier Taiwan LLC. All rights reserved

show abstract

Effect of Bupleuri Radix Extracts on the Toxicity of 5‐Fluorouracil in HepG2 Hepatoma Cells and Normal Human Lymphocytes

Abstract: Despite the excellent chemotherapeutic effect of 5-fluorouracil, its cytotoxicity and genotoxicity in normal cells remain a major problem.

Cited by 43 publications

References 41 publications

Mechanism of 5-Fluorouracil-Induced Apoptosis on Cultured Corneal Endothelial Cells

Mechanism of 5-Fluorouracil-Induced Apoptosis on Cultured Corneal Endothelial Cells

Comparative studies on the genotoxicity and cytotoxicity of polymeric gene carriers polyethylenimine (PEI) and polyamidoamine (PAMAM) dendrimer in Jurkat T-cells

Two Chinese Herbal Regimens Safe for the Elderly on Inhibiting Liver and Bladder Tumor Cell Growth and Regulating Gene Expression

Contact Info

Product

Resources

About