Effect of <i>Cinnamomum osmophloeum</i> Kanehira Leaf Aqueous Extract on Dermal Papilla Cell Proliferation and Hair Growth

Wen, Tung Chou; Li, Yuan Sheng; Rajamani, Karthyayani; Harn, Horng Jyh; Lin, Shinn Zong; Chiou, Tzyy Wen

doi:10.1177/0963689717741139

Cited by 24 publications

(21 citation statements)

References 28 publications

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“…Dermal papilla cells were isolated according to the report of Wen et al (2018) , with some modification. Dorsal skin pieces were digested with Dispase II (0.5 mg/ml; Sigma) at 4°C overnight and incubated at 37°C for additional 30 min.…”

Section: Methodsmentioning

confidence: 99%

Epigallocatechin-3-Gallate Promotes the Growth of Mink Hair Follicles Through Sonic Hedgehog and Protein Kinase B Signaling Pathways

et al. 2018

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Background: Hair follicles play an essential role in the growth of hair. Epigallocatechin-3-gallate (EGCG), a catechin polyphenol in green tea, has various bioactivities. The present study aims to evaluate the effect of EGCG on the growth of mink hair follicles and investigate the possible molecular mechanisms.Methods: The length of hair follicles was recorded up to 6 days in presence of 0.1–5 μM EGCG. Primary dermal papilla cells (DPCs) and outer root sheath cells (ORSCs) were treated with 0.25–4 μM EGCG, and their growth was evaluated by MTT assay and cell cycle detection. The levels of key molecules in sonic hedgehog (Shh) and protein kinase B (AKT) signaling pathways were further assessed by quantitative real-time PCR, western blot and immunofluorescence. To determine the involvement of Shh and AKT pathways in EGCG-mediated growth-promotion of ORSCs and DPCs, Shh pathway inhibitors cyclopamine and GANT61 or AKT pathway inhibitor LY294002 were employed, and then cell proliferation and cell cycle were analyzed.Results: Data from ex vivo culture showed that, in presence of 0.5–2.5 μM EGCG, the growth of mink hair follicles was promoted. In vitro, the proliferation of DPCs and ORSCs was enhanced by 0.5–4 μM EGCG treatment. More cells entered S phase upon treatment of EGCG, accompanied with upregulation of cyclin D1 and cyclin E1. Furthermore, when exposed to EGCG, the Shh and AKT signaling pathways were activated in both hair follicles and primary DPCs and ORSCs. Inhibiting either of these two pathways partly reversed the effect of EGCG on proliferation and cell cycle of DPCs and ORSCs.Conclusion: EGCG promotes the growth of mink hair follicles at concentrations of 0.5–2.5 μM. This growth-promoting effect of EGCG may be associated with the increased proliferation of DPCs and ORSCs through activating Shh and AKT signaling pathways.

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Section: Methodsmentioning

confidence: 99%

Epigallocatechin-3-Gallate Promotes the Growth of Mink Hair Follicles Through Sonic Hedgehog and Protein Kinase B Signaling Pathways

et al. 2018

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“…Terpinenes: Borneol; camphene; camphor; limonene; linalool; myrcene; p-cymene; spathulenol; viridiflorol; b-caryophyllene; b-pinene; d-cadinene; ⍺-cadinol; ⍺pinene; ⍺-terpineol [176] [209] Kaempferol glycosides: [210] Anticancer, [130,211] antidiabetic, [212,213] antidyslipidaemic, [214] antiinflammatory, [209,215] antimicrobial [216,217] , antioxidant, [213,218] cardioprotective, [219] cytotoxicity, [220] hair growth, [221] hepatoprotective, [222] hypolipidaemic effect, [223] hypouricaemic effects, [224] immunomodulatory, [225] larvicidal, [29] pancreas-protective effect, [226] tyrosinase, [220,227] wound healing, [227] xanthine oxidase inhibitory [224] Arthritis, [228] cough and cold, [228] diabetes, [228] infection, [222] inflammation, [228] nerve pains, [228] pyrexia [228] Bark Flavonoids: (E)-Cinnamaldehyde; (Z)cinnamaldehyde. [229] Hydrocarbon: Phenol; c-muurolene [229] Flavonoids: cis-Cinnamaldehyde; transcinnamaldehyde [5,230,231] ; cinnamaldehyde.…”

Section: Leavesmentioning

confidence: 99%

Pharmacological properties and their medicinal uses of Cinnamomum: a review

Kumar

Kumari

Mishra

2019

Journal of Pharmacy and Pharmacology

110

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Objectives Cinnamomum (Family Lauraceae) is traditionally used for flavouring food and in pharmaceutical preparations against various ailments. Detailed literature on the ethnobotanical and pharmacological properties of Cinnamomum is segregated and not present in well-documented form. In the present review, we have been trying to gather its detailed medicinal as well as pharmacological properties. The ethnobotanical and pharmacological properties of Cinnamomum were collected by searching several scientific databases, that is PubMed, Elsevier, Google Scholar, Science Direct and Scopus. Key findings The plant extracts have been reported to possess astringent, warming stimulant, carminative, blood purifier, digestive, antiseptic, antifungal, antiviral, antibacterial, antioxidant, anti-inflammatory and immunomodulatory properties and also help to reduce cholesterol and blood sugar levels. A wide range of phytochemical compounds including aldehydes,

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“…The research on herbs and active constituents as hair growth promoters has been driven by their less toxicity, easy availability, low‐cost, patient compliance and more than one mode of biochemical action . Specific effects of herbal extracts, polyherbal formulations, flavonoids and natural compounds on DPCs have been examined . In vivo hair growth promoting effects as proof of concept are also mentioned.…”

Section: Hair Growth Promoting Effectmentioning

confidence: 99%

Review of Hair Follicle Dermal Papilla cells asin vitroscreening model for hair growth

Madaan

Verma

Singh

et al. 2018

Intern J of Cosmetic Sci

125

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Hair disorders such as hair loss (alopecia) and androgen dependent, excessive hair growth (hirsutism, hypertrichosis) may impact the social and psychological well-being of an individual. Recent advances in understanding the biology of hair have accelerated the research and development of novel therapeutic and cosmetic hair growth agents. Preclinical models aid in dermocosmetic efficacy testing and claim substantiation of hair growth modulators. The in vitro models to investigate hair growth utilize the hair follicle Dermal Papilla cells (DPCs), specialized mesenchymal cells located at the base of hair follicle that play essential roles in hair follicular morphogenesis and postnatal hair growth cycles. In this review, we have compiled and discussed the extensively reported literature citing DPCs as in vitro model to study hair growth promoting and inhibitory effects. A variety of agents such as herbal and natural extracts, growth factors and cytokines, platelet-rich plasma, placental extract, stem cells and conditioned medium, peptides, hormones, lipid-nanocarrier, light, electrical and electromagnetic field stimulation, androgens and their analogs, stress-serum and chemotherapeutic agents etc. have been examined for their hair growth modulating effects in DPCs. Effects on DPCs' activity were determined from untreated (basal) or stress induced levels. Cell proliferation, apoptosis and secretion of growth factors were included as primary end-point markers. Effects on a wide range of biomolecules and mechanistic pathways that play key role in the biology of hair growth were also investigated. This consolidated and comprehensive review summarizes the up-to-date information and understanding regarding DPCs based screening models for hair growth and may be helpful for researchers to select the appropriate assay system and biomarkers. This review highlights the pivotal role of DPCs in the forefront of hair research as screening platforms by providing insights into mechanistic action at cellular level, which may further direct the development of novel hair growth modulators.

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Effect of Cinnamomum osmophloeum Kanehira Leaf Aqueous Extract on Dermal Papilla Cell Proliferation and Hair Growth

Cited by 24 publications

References 28 publications

Epigallocatechin-3-Gallate Promotes the Growth of Mink Hair Follicles Through Sonic Hedgehog and Protein Kinase B Signaling Pathways

Epigallocatechin-3-Gallate Promotes the Growth of Mink Hair Follicles Through Sonic Hedgehog and Protein Kinase B Signaling Pathways

Pharmacological properties and their medicinal uses of Cinnamomum: a review

Review of Hair Follicle Dermal Papilla cells asin vitroscreening model for hair growth

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