Effect of endo‐xylanase‐containing enzyme preparations and laccase on the solubility of rye bran arabinoxylan

Abstract

Three commercial enzyme preparations with endo-xylanase activity, namely Bio-Feed Wheat, Bio-Feed Plus and Grindamyl H 640, and laccase have been tested for their effects on the solubilisation of arabinoxylan (AX) in rye bran or autoclaved rye bran. Autoclaving efficiently increased the availability of AX for enzymatic degradation. Both Bio-Feed Wheat (a monocomponent enzyme) and Bio-Feed Plus (a multicomponent preparation with different enzymatic activities) efficiently degraded the autoclaved rye bran AX into lower-molecular-weight fragments. As much as 70% of the xylose residues and 58% of the arabinose residues in the autoclaved bran were soluble after treatment with Bio-Feed Plus; the weight-average molecular weight of the detectable portion of these soluble polymers was 104 000 Da. Grindamyl H 640 solubilised only a small fraction of the AX in autoclaved rye bran; the molecular weight of these soluble fragments was higher than that of those released by the Bio-Feed xylanases. Addition of laccase during treatment with Bio-Feed Wheat or Grindamyl H 640 decreased the yield of water-soluble AX.

Blasting extrusion processing: The increase of soluble dietary fiber content and extraction of soluble-fiber polysaccharides from wheat bran

“…The results indicate that WBP contains abundant amounts of arabinose, xylose and glucose, and a small amount of galactose with a molar ratio of 0.76:0.99:1:0.12. A similar study suggested that xylose, glucose and arabinose were the main components in autoclaved rye bran (Andersson et al, 2003). Periodate oxidation and smith hydrolysis results revealed the absence of adjacent three hydroxyl carbon-carbon bonds and 1 ?…”

“…4d. Andersson et al found that autoclaved rye bran had a wide polymodal distribution with indications of three major sections with a weight-average molecular weight of 15.93 Â 10 4 Da without any treatment (Andersson, Eliasson, Selenare, Kamal-Eldin, & Åman, 2003). It was reported that water-soluble hemicellulose was extracted and purified from wheat bran with a molecular mass of around 30-35 Â 10 4 Da (Shiiba, Yamada, Hara, Okada, & Nagao, 1993).…”

“…However, the molecular weight of b-D-glucan from wheat bran was decreased to 20 Â 10 4 Da in the presence of some batches of xylanase (Cui, Wood, Blackwell, & Nikiforuk, 2000). When autoclaved rye bran was extensively hydrolyzed by enzyme, the soluble polysaccharides with lower weight-average molecular weights were released (Andersson et al, 2003).…”

Use of endoxylanase treated cereal brans for development of dietary fiber enriched cakes

“…In addition, EX degrade WE-AXs to lower molecular weight, thereby lowering the viscosity involving properties . As far as the degradability of AXs is concerned, it depends on the distribution of arabinose side groups and the amount of disubstitution, as well on the cell wall ultrastructure (Andersson, Eliasson, Selenare, Kamal-Eldin, & Aman, 2003).…”

Engineering Bifunctional Laccase-Xylanase Chimeras for Improved Catalytic Performance

“…These include the reduction in the levels of chlorine-based oxidants in the paper and pulp bleaching process with the consequent reduction in pollutants (17,18) and the use of these enzymes to improve the biochemical and rheological properties of wheat flour, gluten, rye, oats, and other cereals for the baking industry (19,20). Synergism between laccase and xylanase may also benefit the animal feed sector by increasing the digestibility of enzyme-treated lignocellulosic material in the animal diet (21).…”