Effect of Helicobacter pylori infection and its eradication on the expression of tight junction proteins in the gastric epithelium in relation to gastric carcinogenesis

Abstract

Background Tight junction proteins (TJPs) play a role in epithelial defense mechanisms. However, the effect of Helicobacter pylori (Hp) on TJPs remains unclear. This study aimed to evaluate the expression of TJPs in relation to Hp infection and eradication in gastric carcinogenesis. Methods In total, 510 subjects (284 controls and 226 gastric cancer [GC] patients) were prospectively enrolled in the study. The expression of claudin‐1 and ‐2 (CLDN‐1, ‐2), occludin (OCLN), and tight junction protein 1 (TJP1) was measured based on their Hp infection status in normal corpus mucosa and evaluated following Hp eradication using quantitative real‐time polymerase chain reaction (qPCR) and immunohistochemistry (IHC). Results The expression of TJP1 in Hp+ controls was significantly lower than that in Hp− controls (p = 0.006), whereas it was higher in Hp+ than in Hp− GC patients (p = 0.001). Moreover, the increased expression of TJP1 in Hp+ GC patients was reduced to levels in Hp−within a year after Hp eradication and was maintained for more than 5 years. Furthermore, IHC results for TJP1 were similar to qPCR results. In particular, the higher IHC staining intensity of TJP1 in the cytosol of GC patients (p = 0.019) decreased after Hp eradication (p = 0.040). Conclusion Hp infection affects TJP expression. The high expression of TJP1 in Hp+ GC patients was restored to control levels after Hp eradication, suggesting that TJP1 plays a role in gastric carcinogenesis.

Lactobacillus acidophilus CICC 6075 Attenuates HFD-induced Obesity by Improving Gut Microbiota Composition and Histidine Biosynthesis

“…Additionally, HFD-induced obesity reduces Ileibacterium spp., and the abundance of these bacteria is negatively correlated with chronic in ammatory diseases such as obesity, atherosclerosis, and aging [40][41][42]. It has been reported that Helicobacter negatively correlates with intestinal tight junction protein expression, which increases after HFD feeding [43]. Furthermore, we described the association between HFD-induced obesity and controversial Helicobacter, whose abundance did not increase signi cantly after HFD feeding [44,45].…”

Innovative Nomogram Approach to Enhance the Prediction of Helicobacter pylori Infection in Children via Magnetic-Controlled Capsule Endoscopy

“…This retrospective study assembled a cohort of 474 subjects who underwent magnetic-controlled capsule endoscopy coupled with a 13 C urea breath test at the Digestive Endoscopy Center, Children's Hospital a liated with Shanghai Jiao Tong University School of Medicine, spanning January through December 2023. Inclusion criteria encompassed individuals aged 6-18 who had the 13 C urea breath test conducted within a fortnight before or after magnetic-controlled capsule endoscopy. Exclusion criteria included those previously treated for HP infection or those administered proton pump inhibitors, bismuth compounds, or antibiotics within one month prior to the 13 C urea breath test; subjects with a history of gastric surgery; patients with past or present gastric or duodenal ulcers; and cases with incomplete data.…”

“…Diagnosis of Helicobacter pylori (HP) infection within the study cohort was ascertained using the 13 C urea breath test. Participants manifesting positive results were a rmed to harbor HP infection, while those yielding negative outcomes, devoid of any antecedent HP eradication interventions, were adjudged HP-negative.…”

Analysis of Characteristics and Risk Factors of Patients with Single Gastric Cancer and Synchronous Multiple Gastric Cancer among 14,603 Patients

“… 9 TME is related to chronic inflammation and Helicobacter pylori infection of the gastric epithelium is a major risk factor for GC. 10 H. pylori -associated gastritis could promote TME including destruction of tight junction protein. 10 This is supported by the beneficial effects of H. pylori eradication in reducing the risk of primary GC incidence 11 , 12 and prevention effect of metachronous GC with a hazard ratio (HR) of 0.32 (95% confidence interval [CI], 0.15 to 0.66; p=0.002).…”

“… 10 H. pylori -associated gastritis could promote TME including destruction of tight junction protein. 10 This is supported by the beneficial effects of H. pylori eradication in reducing the risk of primary GC incidence 11 , 12 and prevention effect of metachronous GC with a hazard ratio (HR) of 0.32 (95% confidence interval [CI], 0.15 to 0.66; p=0.002). 13 Therefore, we hypothesized that the risk factors and survival rates of SMGC are different from those of single GC.…”