Effect of<i>Lactobacillus plantarum</i>NCU116 on loperamide-induced constipation in mice

Li, Chuan; Nie, Shaoping; Zhu, Ke‐Xue; Xiong, Tao; Li, Chang; Gong, Joshua; Xie, Mingyong

doi:10.3109/09637486.2015.1024204

Cited by 67 publications

(63 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A similar trend of change was observed in HHPD + atropine model test, however, the data failed to reach significant difference, perhaps due to limited sample size ( n = 8 in each group). The result was consistent with the microscopic observation after H&E staining, which also supported that EXQ effectively increased the integrity of colonic mucosa as compared to the vehicle group [29]. …”

Section: Discussionsupporting

confidence: 87%

See 1 more Smart Citation

The immune-regulating effect of Xiao’er Qixingcha in constipated mice induced by high-heat and high-protein diet

Yang

Zheng³

et al. 2017

BMC Complement Altern Med

View full text Add to dashboard Cite

BackgroundXiao’er Qixingcha (EXQ) has been extensively applied to relieve dyspepsia and constipation in children for hundreds of years in China. However, the therapeutic mechanism underlying its efficacy remained to be defined. The present study aimed to clarify the possible laxative and immune-regulating effects of EXQ on two models of experimental constipation in mice, which mimicked the pediatric constipation caused by high-heat and high-protein diet (HHPD).MethodsThe two models of constipated mice were induced by HHPD or HHPD + atropine respectively. To investigate the laxative and immune-regulating activities of EXQ, animals were treated with three doses of EXQ (0.75, 1.5 and 3 g/kg) for 7 consecutive days. The fecal output parameters (number and weight), weight of intestinal content and, the thymus and spleen indexes were measured. The levels of sIgA, IL-10, TNF-α and LPS in colon and serum were determined by ELISA. Furthermore, the pathological changes of colon tissue were examined after routine H&E staining.ResultsBoth HHPD and HHPD + atropine treatments obviously inhibited the fecal output and reduced the colonic sIgA, prominently increased the levels of IL-10 and TNF-α in colonic tissue and elevated the contents of LPS in serum and colonic tissues. In contrast, oral administration of EXQ significantly improved the feces characters and dose-dependently decreased the intestinal changes in both models. In HHPD model test, EXQ efficaciously boosted the sIgA level in a dose-dependent manner, significantly elicited decreases in TNF-α and IL-10 levels, and evidently decreased the spleen and thymus indexes. In HHPD + atropine model test, EXQ treatment reversed the pathological changes by not only dramatically decreasing the spleen index and the levels of LPS and IL-10, but also markedly elevating the thymus index. Furthermore, microscopic observation revealed that EXQ treatment maintained the integrity of colonic mucosa, and protected the colonic tissues from inflammation in the both models.ConclusionsEXQ exhibited prominent laxative activity and effectively protected the colonic mucosal barrier in two models of constipated mice, of which the mechanism might be closely associated with its propulsive and immune-regulating properties. The current results not only validated the rationale for the clinical application of EXQ in pediatric constipation related symptoms, but also threw new light on the immune-inflammatory responses accompanied with chronic constipation pathology.

show abstract

Section: Discussionsupporting

confidence: 87%

“…Then, the stained slides were mounted in neutral balsam and covered with coverslips [29]. Histopathologic changes were observed under optical microscopy (BX53 Olympus, Japan).…”

Section: Methodsmentioning

confidence: 99%

The immune-regulating effect of Xiao’er Qixingcha in constipated mice induced by high-heat and high-protein diet

Yang

Zheng³

et al. 2017

BMC Complement Altern Med

View full text Add to dashboard Cite

show abstract

“…In the case of SD rats, constipation was successfully induced by subcutaneous injection of 3-5mg/kg Lop for 3-6 continuous days in most experiments [8]. However, constipation phenotypes such as decreased stool number and weight in ICR mice were observed after oral administration of 5 mg/kg Lop [1516]. In the present study, we attempted to induce constipation in ICR mice derived from three different sources using Lop injection.…”

Section: Discussionmentioning

confidence: 99%

“…Several strains of rodents have been utilized to conduct most experiments in studies of Lop-induced constipation, including SD rats, Wistar rats, Balb/c mice, ICR mice and ddY mice [811121314], although ICR mice have only been used in two studies [1516]. However, most studies conducted to date have used only one strain of one animal species to produce animal models for constipation and to evaluate the anti-constipation activity of drugs and extracts from herbal medicines.…”

mentioning

confidence: 99%

Characterization the response of Korl:ICR mice to loperamide induced constipation

et al. 2016

View full text Add to dashboard Cite

Animal models of constipation induced with drugs and diet have been widely employed to investigate therapeutic effects and the action mechanism of drugs against this disease. ICR mice were selected to produce this disease model through oral administration of loperamide (Lop), even though SD rats are commonly utilized in studies of constipation. To compare the responses of ICR mice obtained from three different sources to constipation inducers, alterations in stool number, histopathological structure, mucin secretion and opioid-receptor downstream signaling pathway were measured in Korl:ICR (Korea FDA source), A:ICR (USA source) and B:ICR (Japan source) injected with low and high concentrations of Lop (LoLop and HiLop). The number, weight and moisture content of stools decreased significantly in the Lop treated group of all ICR relative to the Vehicle treated group. Additionally, decreased mucosa layer thickness, muscle thickness, and mucin secretion were observed in the transverse colon of Lop treated ICR mice, while a similar number of goblet cells and crypt of lieberkuhn were detected in the same group. Furthermore, a similar change in the level of Gα expression and PKC phosphorylation was detected in the Lop treated group relative to the vehicle treated group, while some differences in the change pattern were observed in the B:ICR group. Therefore, these results of the present study provide strong additional evidence that Korl:ICR, A:ICR and B:ICR derived from different sources have a similar overall response to constipation induced by Lop injection, although there were a few differences in the magnitude of their responses.

show abstract

“…13,14 Although the exact mode of action of probiotics and these potential antibiotic alternatives remains elusive, emerging studies have suggested that probiotics and dietary nutrients are capable of not only modulating gut microbiome, but also modulating immunity at local and systemic levels via the gut-brain-endocrine-immune axis; thereby promoting intestinal health and/or preventing diseases such as IBS. 12,13,[15][16][17] Although there have been many studies evaluating the potential use of probiotics in IBS and/or constipation, 18 limited attempts have been made to incorporate probiotics into chocolate to evaluate potential anti-constipation effects.…”

Section: Introductionmentioning

confidence: 99%

Prophylactic use of probiotic chocolate modulates intestinal physiological functions in constipated rats

et al. 2019

View full text Add to dashboard Cite

BACKGROUND This study investigated the in vivo prophylactic effect of probiotic chocolate on constipation. Rats were administered chocolate containing 2.5 × 1010 CFU g−1 of probiotics daily for 4 weeks and treated with loperamide (5 mg kg−1) daily at the fourth week of treatment. RESULTS Probiotic chocolate treatment significantly (P < 0.05) increased the intestinal motility, colon length, fecal moisture content and number of excreted fecal pellets in constipated rats. Moreover, quantitative real‐time polymerase chain reaction data and histological images also revealed that both probiotic chocolate LYC and BB12 treatments were capable of upregulating the mRNA expression levels of colonic ZO‐1, occludin and AQP8, leading to the maintenance of the defensive barrier function in the constipated rats compared with the negative controls. Interestingly, these treatments also modulated gut bacterial populations by increasing the abundance levels of Lactobacillus and Bifidobacterium, as well as reducing the abundance level of Enterobacteriaceae. CONCLUSION The present study demonstrated that probiotic chocolate LYC and BB12 could potentially be used as alternative agents for prophylactic constipation. © 2018 Society of Chemical Industry

show abstract

Effect ofLactobacillus plantarumNCU116 on loperamide-induced constipation in mice

Cited by 67 publications

References 34 publications

The immune-regulating effect of Xiao’er Qixingcha in constipated mice induced by high-heat and high-protein diet

The immune-regulating effect of Xiao’er Qixingcha in constipated mice induced by high-heat and high-protein diet

Characterization the response of Korl:ICR mice to loperamide induced constipation

Prophylactic use of probiotic chocolate modulates intestinal physiological functions in constipated rats

Contact Info

Product

Resources

About