Effect of Lepidium sativum L. on renal glucose reabsorption and urinary TGF‐β1 levels in diabetic rats

Abstract

The purpose of this study was to determine the mechanism underlying the hypoglycaemic activity of the aqueous extract perfusion of Lepidium sativum L. (LS) in normal and streptozotocin-induced diabetic rats. The aqueous LS extract was administered intravenously and the blood glucose levels were determined within 4 h of treatment. Plasma insulin concentrations and glycosuria were determined. The 24 h urinary transforming growth factor-β β β β β 1 (ELISA) was evaluated in diabetic and control rats 15 days after oral treatment with the aqueous LS extract at a dose of 20 mg/kg.The study showed that LS at a dose of 10 mg/kg/h reduced blood glucose levels both in normal and diabetic rats ( p < < < < < 0.001). At the same time as a potent increase of glycosuria was observed both in normal and diabetic rats (p < < < < < 0.001). In addition, oral administration of LS for 15 days normalized glycaemia (p < < < < < 0.001), enhanced glycosuria ( p < < < < < 0.05 vs diabetic control) and decreased the amount of urinary TGF-β β β β β 1 (p < < < < < 0.01) in diabetic rats. It is concluded that the aqueous LS extract caused a potent inhibition of renal glucose reabsorption which in turn reduced blood sugar. This renal effect is at least one mechanism explaining the observed hypoglycaemic activity of this plant in normal and diabetic rats.

Ethnobotanical Survey of Medicinal Plants Used by Traditional Healers to Treat Diabetes in the Taza Region of Morocco

“…Eddouks et al [ 70 ] showed that the oral administration of C. humilis leaf aqueous extract at 10 mg/kg decreased plasma glucose levels in normal and obese-hyperglycemic-hyperlipidemic (OHH) Meriones shawi rats. Moreover, another study showed that, at the dose of 20 mg/kg, the hypoglycemia effect was also accompanied by the potent inhibition of renal glucose reabsorption [ 71 ]. However, in vitro study using short-circuit current technique revealed that the aqueous extract caused significant inhibition of the electrogenic intestinal absorption of glucose [ 75 ].…”

Complementary and alternative medicine for type 2 diabetes mellitus: Role of medicinal herbs

“…Whole plant inhibits renal glucose reabsorption (Eddouks and Maghrani, 2008) Mangifera indica (mango) Leaves Contains 3β-taraxerol, which enhances insulin induced glucose uptake through translocation of the glucose transporter, GLUT 4 (Sangeetha et al, 2010) Morus indica Leaves Increases tissue glucose uptake and utilization (Andallu et al, 2002) Momordica charantia (Bitter melon, karela)…”

The nutraceutical potential ofLepidium sativum L.seed flavonoid-rich extract in managing metabolic syndrome components

“…Since oxidative stress is highly involved in glucose metabolism imbalance, flavonoids, via their antioxidant action, could also improve glucose tolerance and insulin resistance preventing associated metabolic diseases (Qusti et al, ). Several researchers noted this fact in diabetic rats treated with LS seed powder, aqueous and alcoholic extracts as well as the alkaloid‐rich fraction (Chauhan et al, ; Eddouks & Maghrani, ; Shukla et al, ). The beneficial effect of the LS flavonoid group on carbohydrate metabolism has not been recognized yet.…”