Effect of Veillonella parvula on the antimicrobial resistance and gene expression of Streptococcus mutans grown in a dual‐species biofilm

Luppens, S.B.I.; Kara, Duygu; Bandounas, Luaine; Jonker, Martijs J.; Wittink, Floyd R.A.; Bruning, Oskar; Breit, Timo M.; Cate, J.M. ten; Crielaard, Wim

doi:10.1111/j.1399-302x.2007.00409.x

Cited by 97 publications

(77 citation statements)

References 39 publications

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“…Moreover, the mixed four-species biofilm displayed markedly higher resistance to hydrogen peroxide than any of the singlespecies biofilms. Indeed, numerous studies have demonstrated that multi-species biofilms are generally more resistant to disinfection than mono-species biofilms (Luppens et al 2008;Simoes et al 2009Simoes et al , 2010; Van der Veen and Abee 2010). Unfortunately, the mechanisms involved remain unclear.…”

Section: Pathogen Protection In Multispecies Biofilmsmentioning

confidence: 99%

Resistance of bacterial biofilms to disinfectants: a review

et al. 2011

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A biofilm can be defined as a community of microorganisms adhering to a surface and surrounded by a complex matrix of extrapolymeric substances. It is now generally accepted that the biofilm growth mode induces microbial resistance to disinfection that can lead to substantial economic and health concerns. Although the precise origin of such resistance remains unclear, different studies have shown that it is a multifactorial process involving the spatial organization of the biofilm. This review will discuss the mechanisms identified as playing a role in biofilm resistance to disinfectants, as well as novel anti-biofilm strategies that have recently been explored.

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Section: Pathogen Protection In Multispecies Biofilmsmentioning

confidence: 99%

Resistance of bacterial biofilms to disinfectants: a review

et al. 2011

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“…Moreover, it has become clear that in multispecies biofilms polymicrobial interactions contribute to resistance to antimicrobials and to host immunity [Burmølle et al, 2006;Kara et al, 2006;Luppens et al, 2008;Ramsey and Whiteley, 2009].…”

mentioning

confidence: 99%

Different Response to Amine Fluoride by Streptococcus mutans and Polymicrobial Biofilms in a Novel High-Throughput Active Attachment Model

Exterkate

Crielaard

Cate³

2010

Caries Res

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160

186

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Background/Aims: The antimicrobial resistance of microorganisms in biofilms and the polymicrobial interactions in these biofilms that modulate resistance require novel strategies to evaluate the efficacy of caries-preventive compounds. The current study aimed to evaluate the effects of a caries-preventive agent in Streptococcus mutans and polymicrobial biofilms. Methods: We developed a novel high-throughput active attachment model. The model consisted of a custom-designed lid containing glass discs that fit on top of standard 24-well plates. Biofilms were formed using either S. mutans C180-2 or saliva. At the end of biofilm formation (up to 96 h) the biofilms were treated with amine fluoride (AmF) solutions. The viability of the biofilms was determined by CFU counts, and metabolic activity was measured via lactate production. Results: The effect of AmF on the viability of the polymicrobial biofilms was significantly less than that on the S. mutans biofilms, indicating a higher resistance in the complex biofilms. Both types of biofilms became more resistant to AmF with age. The higher resistance of the polymicrobial biofilms was not reflected in metabolic activity; in dose-response experiments AmF reduced lactate production in both types of biofilms to the same extent. Moreover, the age-induced increased resistance in the polymicrobial biofilms was less pronounced in terms of the inhibition of metabolic activity. Conclusions: This study clearly shows that when evaluating the efficacy of caries-preventive compounds it is essential to use appropriate polymicrobial biofilm models, and more importantly that efficacy needs to be judged based on the reduction of acid formation (i.e. cariogenic potential) as well as on bacterial viability.

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“…This might be of clinical relevance, as P. aeruginosa forms bacterial aggregates in the chronically infected CF lung and it has previously been demonstrated that in oral cavities, V. parvula plays an essential role within multispecies biofilms. where the presence of V. parvula leads to a higher resistance of cocci, such as Streptococcus mutans, against chemical antiseptics (54,55).…”

Section: Discussionmentioning

confidence: 99%

Contribution of Veillonella parvula to Pseudomonas aeruginosa-Mediated Pathogenicity in a Murine Tumor Model System

et al. 2015

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fThe recent finding that high numbers of strict anaerobes are present in the respiratory tract of cystic fibrosis (CF) patients has drawn attention to the pathogenic contribution of the CF microbiome to airway disease. In this study, we investigated the specific interactions of the most dominant bacterial CF pathogen, Pseudomonas aeruginosa, with the anaerobic bacterium Veillonella parvula, which has been recovered at comparable cell numbers from the respiratory tract of CF patients. In addition to growth competition experiments, transcriptional profiling, and analyses of biofilm formation by in vitro studies, we used our recently established in vivo murine tumor model to investigate mutual influences of the two pathogens during a biofilm-associated infection process. We found that P. aeruginosa and V. parvula colonized distinct niches within the tumor. Interestingly, significantly higher cell numbers of P. aeruginosa could be recovered from the tumor tissue when mice were coinfected with both bacterial species than when mice were monoinfected with P. aeruginosa. Concordantly, the results of in vivo transcriptional profiling implied that the presence of V. parvula supports P. aeruginosa growth at the site of infection in the host, and the higher P. aeruginosa load correlated with clinical deterioration of the host. Although many challenges must be overcome to dissect the specific interactions of coinfecting bacteria during an infection process, our findings exemplarily demonstrate that the complex interrelations between coinfecting microorganisms and the immune responses determine clinical outcome to a much greater extent than previously anticipated.

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Effect of Veillonella parvula on the antimicrobial resistance and gene expression of Streptococcus mutans grown in a dual‐species biofilm

Cited by 97 publications

References 39 publications

Resistance of bacterial biofilms to disinfectants: a review

Resistance of bacterial biofilms to disinfectants: a review

Different Response to Amine Fluoride by <i>Streptococcus mutans</i> and Polymicrobial Biofilms in a Novel High-Throughput Active Attachment Model

Contribution of Veillonella parvula to Pseudomonas aeruginosa-Mediated Pathogenicity in a Murine Tumor Model System

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