Effect of<i>Withania somnifera</i>(Ashwagandha) root extract on amelioration of oxidative stress and autoantibodies production in collagen-induced arthritic rats

Khan, Mahmood Ahmad; Subramaneyaan, Mythily; Arora, Vinod; Banerjee, Basu Dev; Ahmed, Rafat S.

doi:10.1515/jcim-2014-0075

Cited by 54 publications

(26 citation statements)

References 25 publications

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“…Ashwaganda has beneficial effects on diseases of cardiovascular nature (Ravindran et al, 2015a), obesity, diabetes, infection, cancer (Choi and Kim, 2015, Khazal et al, 2014, Rai et al, 2016), arthritis (Gupta and Singh, 2014, Khan et al, 2015) and gastric inflammation (Kim et al, 2016). The major constituent in Ashwaganda, identified as Withaferin A, itself is an inhibitor of HMG-CoA, angiotensinogen-converting enzyme, (Ravindran et al, 2015b) can lower total cholesterol, triglycerides, higher HDL/LDL ratios (Shukla et al, 2014) and reduce severity or incidence of myocardial infarction, (Khalil et al, 2015) stroke (Ahmad et al, 2015, Raghavan and Shah, 2015, Sood et al, 2015) and hypertension.…”

Section: Discussionmentioning

confidence: 99%

Natural product HTP screening for attenuation of cytokine-induced neutrophil chemo attractants (CINCs) and NO2− in LPS/IFNγ activated glioma cells

Mazzio

Bauer

Mendonca

et al. 2017

Journal of Neuroimmunology

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Chronic or acute central nervous system (CNS) inflammation is carried out by glial cells, which can contribute to neurological injuries associated with head trauma, stroke, and infection, Parkinson’s or Alzheimer ’s disease. The process of aging combined with inflammation are also risk factors for developing glioblastoma multiforme (GBM) as well as perpetuating its malignant aggression. With growing public curiosity in complementary and alternative medicines (CAMs), in this work we conduct a high throughput (HTP) screening of >1400 natural herbs, plants and over the counter (OTC) products for anti-inflammatory effects on lipopolysaccharide (LPS)/interferon gamma (IFNγ) activated C6 glioma cells. Validation studies suggest a pro-inflammatory profile mediated by LPS [3μg/ml/IFNγ 3ng/ml] is consistent with elevation [> 8.5 fold] of MCP-1 and cytokine-induced neutrophil chemo-attractants (CINC) 1, CINC 2a and CINC3. The data show no evidence of changes to the following, IL-13, TNF-a, fracktaline, leptin, LIX, GM-CSF, ICAM1, L-Selectin, activin A, agrin, IL-1α, MIP-3a, B72/CD86, NGF, IL-1b, MMP-8, IL-1 R6, PDGF-AA, IL-2, IL-4, prolactin R, RAGE, IL-6, Thymus Chemokine-1, CNTF,IL-10 or TIMP-1. The data also show a LPS/IFNγ mediated rise in iNOS and NO2− as often reported. A HTP screening was conducted, where we employ an in vitro efficacy index (iEI) defined as the ratio of toxicity (LC50)/anti-inflammatory potency (IC50) to ensure biological effects were occurring in fully viable cells (ratio > 3.8). Using NO2− as a guideline molecule, the data show that 1.77 % (25 of 1410 tested) (at <250μg/ml) had anti-inflammatory effects with an iEI ratio >3.8. These include reference drugs (hydrocortisone, dexamethasone N6-(1-iminoethyl)-L-lysine and NSAIDS : diclofenac, tolfenamic acid), a histone deacetylase inhibitor (apicidin) and the following natural products; Ashwaganda (Withania somnifera), Elecampagne Root (Inula helenium), Feverfew (Tanacetum parthenium), Green Tea (Camellia sinensis), Turmeric Root (Curcuma Longa) Ganthoda (Valeriana wallichii), Tansy (Tanacetum vulgare), Maddar Root (Rubia tinctoria), Red Sandle wood (Pterocarpus santalinus), Bay Leaf (Laurus nobilis, Lauraceae), quercetin, cardamonin, fisetin, EGCG, biochanin A, galangin, apigenin and curcumin. The herb with the largest iEI was Ashwaganda where the IC50/LC50 was 11.1/>1750.0 μg/mL, and the compound with the greatest iEI was quercetin where the IC50/LC50 was 10.0/>363.6 ug/mL. These substances also downregulate the production of iNOS expression and attenuate CINC-3 release. In summary, this HTP screening provides guideline information as to efficacy of natural products that in the long term, could prevent inflammatory processes associated with neurodegenerative disease and aggressive glioma tumor growth.

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Section: Discussionmentioning

confidence: 99%

Natural product HTP screening for attenuation of cytokine-induced neutrophil chemo attractants (CINCs) and NO2− in LPS/IFNγ activated glioma cells

Mazzio

Bauer

Mendonca

et al. 2017

Journal of Neuroimmunology

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“…The parts of medicinal plant W. somnifera consist of bioactive compounds like withanolides and withaferin A and thereby exhibit antioxidant and anti‐inflammatory property used as antirheumatoid. [ 185,186 ] The preclinical screening concluded that the powder extract of root part tends to have active phytoconstituents attributing an anti‐arthritic effect at a dose of 600 mg/kg and suppressing the rheumatoid symptoms. Therefore, the plant extract can be used as an adjuvant therapy.…”

Section: The Various Herbal Plants Used In the Treatment Of Rheumatoimentioning

confidence: 99%

Medicinal plants used against various inflammatory biomarkers for the management of rheumatoid arthritis

Singh

Mahajan

et al. 2020

Journal of Pharmacy and Pharmacology

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Objectives Rheumatoid arthritis is a chronic autoimmune disease manifested clinically by polyarthralgia associated with joint dysfunction triggering the antibodies targeting against the self‐neoepitopes determined by autoimmune responses associated with chronic arthritic attacks. The activation of macrophages and other defence cells in response to self‐epitopes as biomarkers in RA provides a better understanding of pathogenesis of disease and has led to the development of novel therapeutic approaches acting as potent inhibitors of these cells. Key findings The current review retrieved the various medicinal plants possessing an active phytoconstituents with anti‐inflammatory and antioxidant properties, which tends to be effective alternative approach over the synthetic drugs concerned with high toxic effects. The current available literature provided an evident data concluding that the active constituents like fatty acids, flavonoids, terpenes and sesquiterpene lactones attenuate the RA symptoms by targeting the inflammatory biomarkers involved in the pathogenesis of RA. Summary Despite the various synthetic treatment approaches targeting immune cells, cytokines improved the quality of life but still the drug management is challenging due to toxic and chronic teratogenic effects with anti‐arthritic drugs. The current review has elaborated the selected traditionally used herbal medicinal plants with phytoconstituents possessing anti‐inflammatory activity by suppressing the inflammatory biomarkers with lesser side effects and providing the future exploration of natural drug therapy for rheumatoid arthritis.

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“…The aqueous extract of Withania somnifera Dunal, a common ingredient of antiarthritic polyherbal formulations, reduces the levels of systemic oxidative stress in collagen-induced arthritis in rats: it diminishes the lipid peroxidation levels and glutathione-S-transferase activity and increases the glutathione content in plasma [ 137 ]. This plant extract diminishes the arthritic index and the production of C-reactive protein and antinuclear antibodies in an extent comparable to methotrexate [ 137 ]. The lignan eleutheroside E ((+)-syringaresinol diglucoside), the main active constituent of Acanthopanax senticosus , ameliorates arthritis severity in mice by inhibiting NF- κ B activity, inflammatory cell infiltration, pannus formation, cartilage damage, and bone erosion.…”

Section: The Use Of Natural Products As Therapeutic Adjuvants To Tmentioning

confidence: 99%

Fcγand Complement Receptors and Complement Proteins in Neutrophil Activation in Rheumatoid Arthritis: Contribution to Pathogenesis and Progression and Modulation by Natural Products

Paoliello-Paschoalato

Marchi

Andrade

et al. 2015

Evidence-Based Complementary and Alternative Medicine

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Rheumatoid arthritis (RA) is a highly disabling disease that affects all structures of the joint and significantly impacts on morbidity and mortality in RA patients. RA is characterized by persistent inflammation of the synovial membrane lining the joint associated with infiltration of immune cells. Eighty to 90% of the leukocytes infiltrating the synovia are neutrophils. The specific role that neutrophils play in the onset of RA is not clear, but recent studies have evidenced that they have an important participation in joint damage and disease progression through the release of proteolytic enzymes, reactive oxygen species (ROS), cytokines, and neutrophil extracellular traps, in particular during frustrated phagocytosis of immune complexes (ICs). In addition, the local and systemic activation of the complement system contributes to the pathogenesis of RA and other IC-mediated diseases. This review discusses (i) the participation of Fcγ and complement receptors in mediating the effector functions of neutrophils in RA; (ii) the contribution of the complement system and ROS-dependent and ROS-independent mechanisms to joint damage in RA; and (iii) the use of plant extracts, dietary compounds, and isolated natural compounds in the treatment of RA, focusing on modulation of the effector functions of neutrophils and the complement system activity and/or activation.

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Effect ofWithania somnifera(Ashwagandha) root extract on amelioration of oxidative stress and autoantibodies production in collagen-induced arthritic rats

Abstract: The results showed that WSAq exhibited antioxidant and anti-arthritic activity and reduced inflammation in CIA rats and suggests the potential use of this plant in the treatment of arthritis.

Cited by 54 publications

References 25 publications

Natural product HTP screening for attenuation of cytokine-induced neutrophil chemo attractants (CINCs) and NO2− in LPS/IFNγ activated glioma cells

Natural product HTP screening for attenuation of cytokine-induced neutrophil chemo attractants (CINCs) and NO2− in LPS/IFNγ activated glioma cells

Medicinal plants used against various inflammatory biomarkers for the management of rheumatoid arthritis

Fcγand Complement Receptors and Complement Proteins in Neutrophil Activation in Rheumatoid Arthritis: Contribution to Pathogenesis and Progression and Modulation by Natural Products

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