Effect of IGF2 intron3-g.3072G&gt;A on intramuscular fat (IMF) content in pigs raised in Poland

Oczkowicz, Maria; Tyra, M.; Ropka‐Molik, Katarzyna; Mucha, A.; Żukowski, Kacper

doi:10.1016/j.livsci.2012.06.021

Cited by 15 publications

(16 citation statements)

References 13 publications

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“…For all animals, the following carcass characteristic traits were measured: weight of loin (kg), loin “eye” area (cm) and lean meat percentage (%). Furthermore, several meat quality traits were measured for selected animals: IMF content (the Soxhlet method) [ 20 ] and meat color (redness, a*; yellowness, b*; and lightness, L* parameters; Minolta Chroma Meter CR-310; Osaka, Japan) (699 pigs). The determination of meat color was performed using Chroma Meter CR-310 with a pulse xenon arc lamp D65 and 50 mm orifice.…”

Section: Methodsmentioning

confidence: 99%

Screening for candidate genes related with histological microstructure, meat quality and carcass characteristic in pig based on RNA-seq data

Ropka‐Molik

Bereta

Żukowski

et al. 2018

Asian-Australas J Anim Sci

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ObjectiveThe aim of the present study was to identify genetic variants based on RNA-seq data, obtained via transcriptome sequencing of muscle tissue of pigs differing in muscle histological structure, and to verify the variants’ effect on histological microstructure and production traits in a larger pig population.MethodsRNA-seq data was used to identify the panel of single nucleotide polymorphisms (SNPs) significantly related with percentage and diameter of each fiber type (I, IIA, IIB). Detected polymorphisms were mapped to quantitative trait loci (QTLs) regions. Next, the association study was performed on 944 animals representing five breeds (Landrace, Large White, Pietrain, Duroc, and native Puławska breed) in order to evaluate the relationship of selected SNPs and histological characteristics, meat quality and carcasses traits.ResultsMapping of detected genetic variants to QTL regions showed that chromosome 14 was the most overrepresented with the identification of four QTLs related to percentage of fiber types I and IIA. The association study performed on a 293 longissimus muscle samples confirmed a significant positive effect of transforming acidic coiled-coil-containing protein 2 (TACC2) polymorphisms on fiber diameter, while SNP within forkhead box O1 (FOXO1) locus was associated with decrease of diameter of fiber types IIA and IIB. Moreover, subsequent general linear model analysis showed significant relationship of FOXO1, delta 4-desaturase, sphingolipid 1 (DEGS1), and troponin T2 (TNNT2) genes with loin ‘eye’ area, FOXO1 with loin weight, as well as FOXO1 and TACC2 with lean meat percentage. Furthermore, the intramuscular fat content was positively associated (p<0.01) with occurrence of polymorphisms within DEGS1, TNNT2 genes and negatively with occurrence of TACC2 polymorphism.ConclusionThis study’s results indicate that the SNP calling analysis based on RNA-seq data can be used to search candidate genes and establish the genetic basis of phenotypic traits. The presented results can be used for future studies evaluating the use of selected SNPs as genetic markers related to muscle histological profile and production traits in pig breeding.

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Section: Methodsmentioning

confidence: 99%

Screening for candidate genes related with histological microstructure, meat quality and carcass characteristic in pig based on RNA-seq data

Ropka‐Molik

Bereta

Żukowski

et al. 2018

Asian-Australas J Anim Sci

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“…Samples for IMF content determination were taken from the middle cross sectional area of m. longissimus dorsi behind the last rib. Analysis was performed in thawed muscle homogenates by use Gerhardt SOX 406 (Oczkowicz et al, 2012). The pH was estimated 45 min (pH45) and 24 h (pH24) after slaughter in m. longissimus dorsi (last rib) and m. semimembranosus by use pH-Star (Matthäus, Germany).…”

Section: Measured Traitsmentioning

confidence: 99%

Relationship between VRTN gene polymorphism and growth, slaughter and meat quality traits in three polish pig breeds

et al. 2018

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Earlier studies have shown that insertion/deletion polymorphism (g.20311_20312ins291) in vertnin (VRTN) gene is associated with number of vertebrate in pigs. Because vertebral number determines the size of important meat cuts and influences carcass conformation, the aim of this study was to estimate the relationship between VRTN gene polymorphism and growth, slaughter and pork quality traits including texture parameters. Investigations were carried out on 526 pigs represented three breeds: Polish Landrace, Polish Large White and Puławska. VRTN gene polymorphism was determined by PCR methodology. Association study was performed for each breed separately. It was demonstrated that the analyzed polymorphism had significant influence on several economically important porcine traits. Among growth traits VRTN genotypes were associated with test and lifetime daily gains, number of days on test (p≤0.05) and age of slaughter (p≤0.01, p≤0.05). For carcass parameters, relationships were noticed for carcass yield (p≤0.01), weight of loin and its area, average backfat thickness and weight of primary cuts (p≤0.05). Taking into consideration meat quality, VRTN polymorphism influenced intramuscular fat content, meat colour (yellowness) and pH24 (p≤0.05). For texture parameters, it was correlated with toughness, cohesiveness, chewiness (p≤0.01, p≤0.05) and firmness (p≤0.05) measured in M. longissimus dorsi and M. semimembranosus. Only intramuscular fat content, chewiness of ham, carcass yield and cohesiveness of loin showed unequivocal tendency. In case of the first trait, each breed Wt/Wt genotype was associated with its highest values (p≤0.05), while the second and third traits, Q/Q genotype was correlated with its lowest values (p≤0.01, p≤0.05). Highest loin cohesiveness was observed in animals with Wt/Q genotype (p≤0.01, p≤0.05).

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“…The estimated meat quality features were as follows: meat color (L* -lightness, a* -redness and b* -yellowness parameters; Minolta CR-310 spectrophotometer), pH measured in longissmimus dorsi and semimembranosus muscles at 45 min (pH 45 ) and 24 h (pH 24 ) after slaughter, water holding capacity -WHC (the Graua-Hamma method), and intramuscular fat content -IMF (the Soxhlet method) (Oczkowicz et al, 2012).…”

Section: Animalsmentioning

confidence: 99%

Polymorphisms of the membrane-associated ring finger 4, ubiquitin protein ligase gene (MARCH4) and its relationship with porcine production traits

et al. 2015

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Effect of IGF2 intron3-g.3072G>A on intramuscular fat (IMF) content in pigs raised in Poland

Cited by 15 publications

References 13 publications

Screening for candidate genes related with histological microstructure, meat quality and carcass characteristic in pig based on RNA-seq data

Screening for candidate genes related with histological microstructure, meat quality and carcass characteristic in pig based on RNA-seq data

Relationship between VRTN gene polymorphism and growth, slaughter and meat quality traits in three polish pig breeds

Polymorphisms of the membrane-associated ring finger 4, ubiquitin protein ligase gene (MARCH4) and its relationship with porcine production traits

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