The research was aimed at determining the physicochemical and sensory properties of the m. longissimus lumborum at DanAvl Hybrid (Landrace – Yorkshire) fatteners and derived from DanAvl Hybrid sows and DanAvl Duroc and Pulawska boars. Fatteners derived from DanAvl Duroc boars were characterized by better meat quality compared to DanAvl Hybrids and their hybrids with Pulawska breed as evidenced by higher pH in 24–96 h post mortem (p.m.), higher sensory evaluation of juiciness, tenderness and flavor as well as lower shear force at lower content of total protein and higher content of intramuscular fat (IMF). However, DanAvl Hybrid fatteners were characterized by poorer meat quality, i.e. higher color lightness (L*) and higher drip loss both from fresh and thawed meat compared to those derived from DanAvl Duroc and Pulawska boars. Meat of fatteners after Pulawska breed was characterized by the highest redness (a*), and that after DanAvl Duroc breed, the lowest yellowness (b*) and saturation (C*).
The aim of this study was to estimate the relationship between prolactin receptor (PRLR) gene polymorphism and litter size in pigs of synthetic line 990 (n = 374). Prolactin receptor gene variants were determined by PCR-RFLP method. It was demonstrated that the analyzed polymorphism had significant influence (P ≤ 0.05) on reproduction traits in the first parity.
the aim of this study was to analyze the association of polymorphisms in alpha-ketoglutarate-dependent dioxygenase (FTO) and perilipin 2 (PLIN2) genes with carcass and meat quality traits in pigs reared in poland. the research covered 578 sows that belong to the following breeds: Duroc, Hampshire, Polish Landrace, Pietrain, Puławska and Polish Large White. FTO (fm244720:g.400c>g) and PLIN2 (gu461317:g.98g>A) genes variants were determined by means of pcr-rflp and Acrs-pcr methods respectively. Association between individual genotypes and analyzed traits was calculated by means of glm procedure for polish landrace, polish Large White and Puławska breeds separately and for all six breeds together in case of FTO gene. the results showed that FTO variants were associated with weight of loin without backfat and skin (WL), loin eye area (AL) and meat percentage (MP) in Polish Large White (P≤0.05), mean backfat thickness from 5 measurements (Bft) and ph measured 45 min after slaughter in m. longissimus dorsi (pH24 ld) as well as with water holding-capacity (WHC) in Puławska breed (P≤0.01). PLIN2 genotypes, however were correlated with WL and height of the loin eye (HL) in Polish Large White and Puławska (P≤0.05), AL in Polish Large White (P≤0.01) as well as luminosity (L*) in Puławska (P≤0.05) pigs. We observed most consistent relationships of PLIN2 snp with intramuscular fat content (IMF) and WHC. In 3 analyzed breeds GG genotype was connected with highest values of these traits (P≤0.05).
Estrogen receptor gene (ESR) which is localized in the swine chromosome 1, has been recognized as a "candidate" gene of reproductive traits. The aim of this study was to determine the mutations in the ESR/AvaI (ESR1) and ESR/PvuII (ESR2) gene in boars kept at the AI Station and its effect on selected quantitative and qualitative characters of the semen. The study included 217 boars maintained at the AI Station. The ESR genotypes were determined with PCR-RFLP. Two alleles ESR1 were identified: A (109 and 76 bp), B (76, 62 and 47 bp) and also two alleles von ESR2. Die ermittelten Ergebnisse wiesen eine signifikante (P≤0,01) Überlegenhei der Eber mit den Genotypen ESR1 und ESR2 hinsichtlich alles untersuchten Spermamerkmale auf.
The aim of the experiment was to detect polymorphism in the GPX5 gene and to determine associations between individual genotypes and selenium concentration in liver and kidney of wild boars from West Pomerania province in Poland. The polymorphism in GPX5 gene was detected using the PCR-RFLP method with specific primers and the restriction enzyme HinfI. Two different alleles of the GPX5 gene were identified -allele 1B (0.37) and 2B (0.63). The relationships between the GPX5 genotypes and concentrations of selenium in liver and kidney were analyzed, revealing statistically significant (P≤0.05) differences between wild boars carrying different GPX5 genotypes in both genders. Obtained results indicated that wild boars with the 2B2B genotype had the highest selenium concentration in livers and kidneys compared to 1B1B and 1B2B animals and these differences were statistically significant (P≤0.05).
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