Effect of immobilization technique on performance ZnO nanorods based enzymatic electrochemical glucose biosensor

Abstract

Abstract:In this paper, ZnO Nanorods (ZNR) have been synthesized over Platinum (Pt) coated glass substrate with in-situ addition KMnO 4 during hydrothermal growth process. Significant variation in ZnO nanostructures was observed by KMnO 4 addition during the growth. Glucose oxidase was later immobilized over ZNRs. The asprepared ZNRs were further utilized for glucose detection by employing amperometric electrochemical transduction method. In order to optimize the performance of the prepared biosensor two different immobilization techniques i.e. physical adsorption and cross linking have been employed and compared. Further investigations suggest that immobilization via cross linking method resulted in the improvement of the biosensor performance, thereby significantly affecting the sensitivity and linear range of the fabricated biosensor. Among the two types of biosensors fabricated using ZNR, the best performance was shown by cross linked electrodes. The sensitivity for the same was found to be 17.7 mA-cm -2 -M -1 , along with a wide linear range of 0.5-8.5 mM.

Progress of Enzymatic and Non-Enzymatic Electrochemical Glucose Biosensor Based on Nanomaterial-Modified Electrode

“…They reported that APS gave the highest sensitivity of 17.72 μAcm −2 mM −1 compared with other samples because of high GOx enzyme loading in APS coupling agents and low electron transfer resistance for efficient electrocatalytic activity with glucose. Shukla et al [ 102 ] studied the glucose sensor performance via two different immobilization methods (physical adsorption and cross-linking). They found that the sensitivity and linear range of sensors improve when the cross-linking method is used, which is due to an increase in enzyme loading onto the ZnO nanorod (NR) surface and less enzyme leaching compared with the physical adsorption immobilization.…”