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Abstract:In this paper, ZnO Nanorods (ZNR) have been synthesized over Platinum (Pt) coated glass substrate with in-situ addition KMnO 4 during hydrothermal growth process. Significant variation in ZnO nanostructures was observed by KMnO 4 addition during the growth. Glucose oxidase was later immobilized over ZNRs. The asprepared ZNRs were further utilized for glucose detection by employing amperometric electrochemical transduction method. In order to optimize the performance of the prepared biosensor two different immobilization techniques i.e. physical adsorption and cross linking have been employed and compared. Further investigations suggest that immobilization via cross linking method resulted in the improvement of the biosensor performance, thereby significantly affecting the sensitivity and linear range of the fabricated biosensor. Among the two types of biosensors fabricated using ZNR, the best performance was shown by cross linked electrodes. The sensitivity for the same was found to be 17.7 mA-cm -2 -M -1 , along with a wide linear range of 0.5-8.5 mM.

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