Effect of in-ovo injection of glucose and egg white protein on the production performance and gut histomorphometry of broiler chicken

Abstract

A biological trial was carried out to investigate the effect of in ovo injection of glucose and egg white protein on the production performance and gut histomorphometry of broilers. 500 hatching eggs with uniform weight (68±1g) were randomly allotted to five treatment groups each with five replicates of 20 eggs each and incubated. The eggs were injected with one of the following nutrient solutions on 18th day of incubation viz. 0.5 ml of 25 % glucose (T3), 0.5 ml of 12% egg white protein (T4), 0.5 ml of 25 % glucose + egg white protein 12% (T5) into the amnion along this100 eggs served as non injected control (T1) and 100 eggs injected with normal saline (T2) which also served as sham control. The hole was sealed and the period of incubation was continued till 21st day. After hatching, 400 chicks were randomized in to five treatments of five replicates each with 16 chicks. Data on hatchability, hatch weight, bi-weekly body weight, weight gain, feed consumption, FCR and villi height, width, crypt depth of duodenum, jejunum and ileum were recorded and analysed statistically for significant difference. Results revealed that in ovo injection of glucose, egg white protein and their combination significantly (P less than 0.05) increased hatchability, chick weight, body weight and livability. However, the feed consumption was significantly (P less than 0.01) higher in glucose, egg white protein and glucose + egg white protein injected birds compared to non injected control and sham groups. The FCR was significantly (P less than 0.05) improved in all in ovo injected groups compared to control and sham. In ovo feeding significantly (P less than 0.05) increased the duodenal, jejuna and ileal villi height, width and crypt depth (P less than 0.01) compared to control birds. In ovo injection of glucose and EWP either alone or in combination improved the productive performance, gut histomorphology and net profit of the broilers.

Impact of In-Ovo Injection of Folic Acid and Glucose on Hatchability and Post-Hatching Performance of Broiler Chicken

“…Salmanzadeh (2012) reported that the in-ovo injection of 0.5 ml of 25 % glucose into the albumen on day seven of incubation did not affect feed intake. Kanagaraju and Rathnapraba (2019) found that the treatment of in-ovo injection of 0.5 ml of 25 % glucose on day 18 of incubation into the amnion significantly increased feed intake compared to the negative control and positive groups. This discrepancy in the results might be due to the differences in injection time.…”

“…reported that body weight was significantly increased by the age of 42 days. Salmanzadeh (2012), Kanagaraju and Rathnapraba (2019) also indicated that in-ovo injection with 0.5 ml of 25 % glucose (on day-7 of incubation in the albumen and on day-18 of incubation in the amnion, respectively) improved body weight. Zhai et al (2011c) found that injected chicken embryo in the amnion on day 19 of incubation with 0.1, 0.4, 0.7, or 1.0 mL of various carbohydrates (Glucose, fructose, sucrose, maltose, and dextrin) associated with chicken's body weight.…”

“…An increases in the body weight of in-ovo-injected broiler embryos with (FA + Glu) could be viewed as a consequence of the improvement in enteric development and a subsequent enhancement in nutrient absorption (Zhai et al 2011c), or as a good nutrient for better use of the energy by the embryos Uni et al (2005). Kanagaraju and Rathnapraba (2019) also stated that the in-ovo injection of 0.5 ml of 25 % glucose into the amnion on day 18 of incubation significantly improved duodenal, jejunal and ileal histomorphology (villi height, width, crypt depth, and villi surface area) of broilers, which led to the enhancement of digestion and absorption of nutrients. On other hand, Zhang et al (2016) indicated that the individual injection of 0.4 mL glucose (25 mg) on day 18 of the incubation did not affect hatching weight and growth performance of the chickens during the first week of post-hatching.…”

“…However, Nouri et al (2018) stated that the FCR in broilers that had been injected with 120 µg folic acid in albumen on day 7 of incubation was significantly improved on 0-42 days compared to the control treatment. On the other hand, Salmanzadeh (2012), Kanagaraju and Rathnapraba (2019) found that chickens that were injected with glucose in-ovo had a higher FCR than chickens hatched from the control group and the positive group.…”

Effect of In Ovo Ornithine-á-Ketoglutarate Feeding on Early Body Weight and Pectoral Muscle Development of Chicks

“…Due to the streamlined nature of many metabolic processes, the lack of nutrients in the later stages of embryonic development may have long-term irreversible effects on poultry. In ovo feeding involves the injection of exogenous nutrients into hatching eggs, which can correct the nutrient deficiency at the later stages of embryo development (Kanagaraju and Rathnapraba, 2019) and promote the growth and development of poultry during incubation and after hatching (Al-Daraji et al, 2012;Bhanja and Mandal, 2005;Coskun et al, 2017;Ohta and Kidd, 2001;Salmanzadeh, 2012;Uni and Farket, 2004).…”