Effect of Incubation Temperature on Antibiotic Resistance and Virulence Factors of Acinetobacter baumannii ATCC 17978

Abstract

is a notorious opportunistic pathogen that is prevalent mainly in hospital settings. The ability of to adapt and to survive in a range of environments has been a key factor for its persistence and success as an opportunistic pathogen. In this study, we investigated the effect of temperature on the clinically relevant phenotypes displayed by at 37°C and 28°C. Surface-associated motility was significantly reduced at 28°C, while biofilm formation on plastic surfaces was increased at 28°C. Decreased susceptibility to aztreonam and increased susceptibility to trimethoprim-sulfamethoxazole were observed at 28°C. No differences in virulence, as assayed in a model, were observed. Proteomic analysis showed differential expression of 629 proteins, of which 366 were upregulated and 263 were downregulated at 28°C. Upregulation of the Csu and iron uptake proteins at 28°C was a key finding for understanding some of the phenotypes displayed by at 28°C.

<em>Acinetobacter baumannii</em> biofilms: effects of physicochemical factors, virulence, antibiotic resistance determinants, gene regulation, and future antimicrobial treatments

“…40 A report demonstrated that A. baumannii strains formed biofilm better at 30°C than at 37°C while another showed that biofilm formation on plastic surfaces was high at 28°C due to the up-regulation of certain biofilm-associated proteins (Baps) such as Csu and iron-uptake proteins in A. baumannii . 39 …”

Coping with low water activities and osmotic stress in Acinetobacter baumannii: significance, current status and perspectives

“…A. baumannii is by now suggested to be responsible for 2%–10% of all nosocomial infections worldwide (Antunes et al ., ; De Silva et al ., ; Wong et al ., ; Harding et al ., ). Primarily patients in intensive care units (ICUs) suffer from the opportunistic pathogen, which can cause, amongst others, pneumonia, sepsis, meningitis and infections of the urinary tract or wounds (Allen and Green, ; Dijkshoorn et al ., ; Peleg et al ., ; Howard et al ., ; McConnell et al ., ).…”

The UDP‐GalNAcA biosynthesis genes gna‐gne2 are required to maintain cell envelope integrity and in vivo fitness in multi‐drug resistant Acinetobacter baumannii

“…Considering the potential increase in PNAG production by the a/e2 mutant, it was not surprising to observe that the ∆a/e2 mutant developed a rdar-like morphotype on CR plates under conditions typically unfavorable to biofilm formation ( Fig. 3C) (37°C, unpublished data and (De Silva et al, 2018)). Complementation of the ∆a/e2 mutant restored the WT phenotype, confirming the role of gna-gne2 in biofilm formation.…”