Effect of Indole Acetic Acid on Oxygen Metabolism in Cultured Rat Neutrophil

Melo, Mariza Pires de; Curi, TaniaCristinaPithon; Miyasaka, Célio Kenji; Palanch, A C; Curi, Rui

doi:10.1016/s0306-3623(98)00032-9

Cited by 44 publications

(44 citation statements)

References 22 publications

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“…Urea is the end-product of the protein catabolism and this is confirmed by the decrease in plasma proteins and referred to kidney dysfunction as proven by enlargement of the relative weight of kidney as suggested by Yousef et al (2003). Additionally, the recorded oxidative stress that is indicated by the current elevation in MDA due to GA 3 administration is an agreement with various studies (De Melo et al 1998;Soliman et al 2010). This elevation in MDA may be due to that GA 3 is associated with generation of ROS which interacts with tissues leading to numerous pathophysiological alterations (Yu et al 2008).…”

Section: Discussionsupporting

confidence: 73%

Grape seeds proanthocyanidin extract as a hepatic-reno-protective agent against gibberellic acid induced oxidative stress and cellular alterations

Hassan

Al-Rawi

2012

Cytotechnology

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The present study aims to investigate the heptonephro-protective effect of grape seeds proanthocyanidin extract (GSPE) against the risks induced by gibberellic acid (GA3) in male rats. The results recorded that GA3 caused a significant increase in total lipids, total cholesterol, triglycerides and LDL-C levels in serum, concomitant with a significant decrease in serum HDL-C. A significant increase in serum AST, ALT, urea and creatinine, while, a significant decrease in total protein content in serum was observed in rats given GA3. Hepatic and renal lipid peroxidation product (MDA) was significantly increased, meanwhile, total antioxidant capacity (TAC), glutathione, and catalase levels were significantly decreased. In addition, there was a negative change in liver structure including dilatation in the central veins with degeneration of endothelium cells and cellular injury around the veins as well as in the kidney structure such as lesion in both glomeruli and tubules, detachment of the Malpighian corpuscles from the Bowman's capsule's epithelium, shrinkage in the glomerular capillary network. However, almost all of these adverse effects seemed to be ameliorated by oral administration of GSPE with GA3 to rats for 2 month indicating the protective effect of grape seeds GSPE on GA3 induced oxidative stress in rats.

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Section: Discussionsupporting

confidence: 73%

Grape seeds proanthocyanidin extract as a hepatic-reno-protective agent against gibberellic acid induced oxidative stress and cellular alterations

Hassan

Al-Rawi

2012

Cytotechnology

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“…The cell suspension was centrifuged at 4 C (500 g for 10 min). The number of viable cells (>95% neutrophils) was determined in a Neubauer chamber under an optical microscope by Trypan blue exclusion (Pires de Melo et al 1998, Pithon-Curi et al 2002.…”

Section: Methodsmentioning

confidence: 99%

Diabetes causes marked changes in function and metabolism of rat neutrophils

Alba-Loureiro

Hirabara²,

Mendonça³

et al. 2006

Journal of Endocrinology

152

133

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Several studies have shown impairment of neutrophil function, a disorder that contributes to the high incidence of infections in diabetes. Since glucose and glutamine play a key role in neutrophil function, we investigated their metabolism in neutrophils obtained from the peritoneal cavity of streptozotocin-induced diabetic rats. The activities of hexokinase, glucose-6-phosphate dehydrogenase (G6PDH)

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“…15 Horseradish peroxidase isozyme C (HRPC) oxidizes IAA to produce free radicals that are cytotoxic to mammalian cells. [16][17][18][19] IAA or HRP alone shows no cytotoxic effect in mammalian cells. Only the combination of these two compounds can result in cell death.…”

Section: Introductionmentioning

confidence: 95%

“…Only the combination of these two compounds can result in cell death. [16][17][18][19] One strategy to selectively induce tumor cell death and avoid damage to normal tissues is to target HRP on tumor cells for producing the toxic IAA metabolites. As the high level of alpha-fetoprotein (AFP) is expressed in liver tumor cells, but not in adult normal liver cells, 20,21 AFP has been chosen as a promoter for liver tumor-targeted gene delivery.…”

Section: Introductionmentioning

confidence: 99%

Tumor-targeted gene therapy using Adv-AFP-HRPC/IAA prodrug system suppresses growth of hepatoma xenografted in mice

et al. 2011

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Clinical efficacy of current therapies for hepatocellular carcinoma (HCC) treatment is limited. Indole-3-acetic acid (IAA) is non-toxic for mammalian cells. Oxidative decarboxylation of IAA by horseradish peroxidase (HRP) leads to toxic effects of IAA. The purpose of this study was to investigate the effects of a novel gene-targeted enzyme prodrug therapy with IAA on hepatoma growth in vitro and in vivo mouse hepatoma models. We generated a plasmid using adenovirus to express HRP isoenzyme C (HRPC) with the HCC marker, alpha-fetoprotein (AFP), as the promoter (pAdv-AFP-HRPC). Hepatocellular cells were infected with pAdv-AFP-HRPC and treated with IAA. Cell death was detected using MTT assay. Hepatoma xenografts were developed in mice by injection of mouse hepatoma cells. The size and weight of tumors and organs were evaluated. Cell death in tumors was assessed using hematoxylin and eosin-stained tissue sections. HRPC expression in tissues was detected using Reverse Transcriptase-Polymerase Chain Reaction. IAA stimulated death of hepatocellular cells infected with pAdv-AFP-HRPC, in a dose-and time-dependent manner, but not in control cells. Growth of hepatoma xenografts, including the size and weight, was inhibited in mice treated with pAdv-AFP-HRPC and IAA, compared with that in control group. pAdv-AFP-HRPC/IAA treatment induced cell death in hepatoma xenografts in mice. HRPC gene expressed only in hepatoma, but not in other normal organs of mice. pAdv-AFP-HRPC/IAA treatment did not cause any side effects on normal organs. These findings suggest that pAdv-AFP-HRPC/IAA enzyme/prodrug system may serve as a strategy for HCC therapy.

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Effect of Indole Acetic Acid on Oxygen Metabolism in Cultured Rat Neutrophil

Cited by 44 publications

References 22 publications

Grape seeds proanthocyanidin extract as a hepatic-reno-protective agent against gibberellic acid induced oxidative stress and cellular alterations

Grape seeds proanthocyanidin extract as a hepatic-reno-protective agent against gibberellic acid induced oxidative stress and cellular alterations

Diabetes causes marked changes in function and metabolism of rat neutrophils

Tumor-targeted gene therapy using Adv-AFP-HRPC/IAA prodrug system suppresses growth of hepatoma xenografted in mice

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