Effect of inducers on metabolism of benzo(a)pyrene invivo and invitro: Analysis by high pressure liquid chromatography

Datta, Debjani; Samanta, Timir B.

doi:10.1016/s0006-291x(88)81114-8

Cited by 20 publications

(7 citation statements)

References 16 publications

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“…After filtration and centrifugation, aliquots of 100 l were used to determine the radioactivity of the aqueous ethanolic extracts. The used filters including the fungal biomass were burned in a combustion chamber (Junitek, Turku, Finland) to determine the amount of bound 14 C as evolving 14 CO 2 . The distribution of radioactivity in the ethanol extracts was analyzed by HPLC using the Merck RP-18 column and the conditions described above.…”

Section: Methodsmentioning

confidence: 99%

“…The effect of Tween 20 and Tween 80 on the MnP-catalyzed conversion was studied by using [ 14 MnP 1 oxidized the labeled BaP in the presence of both surfactants, considerable differences were observed depending on the particular type of Tween (Fig. 5B, D (Fig.…”

Section: Conversion Of Bap By Whole Fungal Cultures S Coronilla Conmentioning

confidence: 99%

“…The crude MnP was more efficient than the purified MnP 1, which could be attributed either to additional activities in the crude enzyme (other MnPs or laccase) or to its higher stability. Fungal metabolism of BaP has been shown for several molds (deuteromycetes and zygomycetes), among others Aspergillus ochraceae (14), Cunninghamella elegans (11), and a Penicillium sp. (32), which hydroxylate BaP via cytochrome P-450-dependent mono-oxygenases (1).…”

Section: Conversion Of Bap By Whole Fungal Cultures S Coronilla Conmentioning

confidence: 99%

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Degradation of Benzo[ a ]pyrene by the Litter-Decomposing Basidiomycete Stropharia coronilla : Role of Manganese Peroxidase

Steffen

Hatakka

Hofrichter

2003

Appl Environ Microbiol

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The litter-decomposing basidiomycete Stropharia coronilla, which preferably colonizes grasslands, was found to be capable of metabolizing and mineralizing benzo Benzo[a]pyrene (BaP), a polycyclic aromatic hydrocarbon (PAH) consisting of five fused benzene rings, is known to be carcinogenic and mutagenic (10,12). Together with other PAHs, BaP is commonly formed during the pyrolysis and incomplete combustion of biological material and organic compounds and is found in various concentrations in coal tar, petroleum, and oil-based fuels (42). Thus, soils from gasworks sites and carbochemical plants as well as refineries and filling stations are often contaminated with BaP. This causes an obvious health risk that has raised the public interest in the fate and the removal of BaP and similar compounds in and from the environment.PAHs with more than four rings are considered highly recalcitrant and resistant to microbial degradation (10). In addition, the higher their molecular weight is, the lower is their water solubility and thus their bioavailability (54). While lowermolecular-mass PAHs-e.g., naphthalene, anthracene, and phenanthrene-are readily degraded by a number of aerobic bacteria, which can utilize these compounds as carbon sources (10,12,33), BaP and other PAHs with high molecular masses are cometabolically degraded by only a few bacterial species (Mycobacterium spp. and Sphingomonas spp.) and mixed microbial cultures (33).In addition to certain bacteria, wood-colonizing basidiomycetes such as Phanerochaete chrysosporium and Bjerkandera sp. strain BOS55 have been shown to metabolize BaP in liquid culture and soil (9, 24, 36). These white-rot fungi produce extracellular ligninolytic oxidoreductases, namely, manganese peroxidase (MnP), lignin peroxidase (LiP), and laccase, which attack aromatic substances, including PAHs, via the formation of free radicals (25,26,35). Since these fungi specialize in colonizing compact wood (timber and stumps) and cannot compete in soil for a prolonged time, their actual contribution to the removal of recalcitrant PAHs under natural conditions seems to be limited. There is, however, a second ecophysiological group of ligninolytic basidiomycetes-the litter-decomposing fungi-which have recently been shown to possess a ligninolytic enzyme system similar to that of white-rot fungi (48,49). Furthermore, screening tests have demonstrated that litter-decomposing fungi are capable of metabolizing PAHs, including BaP, to some extent (21,22,47,56).In the present study, we report the degradation of BaP by the litter-decomposing fungus Stropharia coronilla, which preferably inhabits grasslands. The species is a typical grass dweller that colonizes pastures, meadows, and waysides and is found in both Europe and North America (8,40). Particular attention is paid to the role of MnP, the predominant ligninolytic enzyme of this fungus, and the results indicate its crucial role in the degradation of PAH. MATERIALS AND METHODSOrganism, culture conditions, and enzyme preparation. The litter-decompos...

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Section: Methodsmentioning

confidence: 99%

Section: Conversion Of Bap By Whole Fungal Cultures S Coronilla Conmentioning

confidence: 99%

Section: Conversion Of Bap By Whole Fungal Cultures S Coronilla Conmentioning

confidence: 99%

See 1 more Smart Citation

Degradation of Benzo[ a ]pyrene by the Litter-Decomposing Basidiomycete Stropharia coronilla : Role of Manganese Peroxidase

Steffen

Hatakka

Hofrichter

2003

Appl Environ Microbiol

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“…In our study, BaP and pyrene were activated to form many products in the PN/T( p-Cl)PPFeCl model system. We identified their main metabolites and found that, besides the same alcohols and aldehydes as the enzymatic reaction, we could also identify the formation of the specific metabolites nitrobenzo[a]pyrene and nitropyrene [27,28]. Presently, the origin and the metabolic process of nitro-BaP in the environment are still unclear [29].…”

Section: Discussionmentioning

confidence: 99%

Production of polycyclic aromatic hydrocarbon metabolites from a peroxynitrite/iron(III) porphyrin biomimetic model and their mutagenicities

Luo

Dai

Zhong

et al. 2010

Enviro Toxic and Chemistry

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Some polycyclic aromatic hydrocarbons (PAHs) are typical promutagens that require metabolic activation to exhibit their mutagenicities and carcinogenicities. The metabolites of three PAHs, pyrene (PY), fluoranthene (FLU), and benzo[a]pyrene (BaP), produced from the peroxynitrite/T(p-Cl)PPFeCl(peroxynitrite/(chloride)iron(III)tetrakis(p-chlorophenyl)porphyrin) system, have been identified with high-performance liquid chromatography coupled with electron spray ionization tandem mass spectrometry. The results demonstrated that three major metabolites were the quinone group, OH group, and nitro group. In the Ames test, all three PAH metabolites became mutagenic without using the enzymatic activating system, whereas their parents did not show positive results. Cell transformation assay indicated that 1,3-nitro-BaP and BaP metabolites produced from this biomimetic system have more serious effects in inducing cancer than the BaP parent.

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“…Biological degradation of PAHs compounds often results, incomplete degradation, production and accumulation of intermediate products. Intermediate metabolites including dihydrodiols, phenols and areneoxides have been identified as carcinogen and mutagen pose greater risk to the environment than the parent compounds due to their increased polarity, solubility and mobility (Datta and Samanta 1988). In India, oil refineries environment contaminated with PAHs due to poor management, faulty equipment, vehicle exhaust and lack of environmental controls may have significant impact on surrounding environment.…”

Section: Introductionmentioning

confidence: 99%

Isolation of pyrene degrading Achromobacter xylooxidans and characterization of metabolic product

Tiwari

Reddy

Patel

et al. 2010

World J Microbiol Biotechnol

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Polycyclic aromatic hydrocarbons (PAHs) are common ubiquitous pollutants existing in nature with high recalcitrance and toxicity. In this study a bacterium capable of aerobic degradation of high molecular weight PAHs (with special reference to pyrene) was isolated by selective enrichment culture technique from oil refinery effluent sludge. The isolate was characterized as Achromobacter xylooxidans by 16S rRNA gene sequence analysis technique. For the first time it is hereby reported a bacterium capable of effectively degrading pyrene (up to 80%), as evident by reverse phase high performance liquid chromatographic analysis (RP-HPLC). After incubation of Achromobacter xylooxidans in minimal salt medium (MSM) containing pyrene, at concentration of 200 mg/L, as sole source of carbon and energy, there was decrease in pyrene concentration concomitant with increase in bacterial cell protein concentration. RP-HPLC analysis revealed that pyrene was degraded into three metabolites viz. I, II and III. The RP-HPLC eluent fraction were collected from 2.5 to 32.0 min by repeated injection of degraded sample, concentrated and analyzed on gas chromatography mass spectroscopy (GC-MS) for metabolite identification. The fraction shows 1-hydroxypyrene, 1-hydroxy-6-methoxypyrene and 1,6dimethoxypyrene as metabolic product of pyrene degradation, on the basis of their m/z values. On contrary to the reported PAH degradation with reference to pyrene by different isolates till date; the efficient degradation, as evident by RP-HPLC, by this isolate holds a promising potential for planning of bioremediation strategies of contaminated sites.

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Effect of inducers on metabolism of benzo(a)pyrene invivo and invitro: Analysis by high pressure liquid chromatography

Cited by 20 publications

References 16 publications

Degradation of Benzo[ a ]pyrene by the Litter-Decomposing Basidiomycete Stropharia coronilla : Role of Manganese Peroxidase

Degradation of Benzo[ a ]pyrene by the Litter-Decomposing Basidiomycete Stropharia coronilla : Role of Manganese Peroxidase

Production of polycyclic aromatic hydrocarbon metabolites from a peroxynitrite/iron(III) porphyrin biomimetic model and their mutagenicities

Isolation of pyrene degrading Achromobacter xylooxidans and characterization of metabolic product

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