Effect of inhibitors of two isoprenoid biosynthetic pathways on physiological and biosynthetic characteristics of Dioscorea deltoidea cell suspension culture

Suspension Cell Culture of Dioscorea deltoidea—A Renewable Source of Biomass and Furostanol Glycosides for Food and Pharmaceutical Industry

“…accumulate predominantly SG of the furostanol type [47]. The content and ratio of individual glycosides in cell culture vary significantly depending on both physical (gas exchange, temperature, bioreactor type) and chemical (medium composition, growth regulators) factors [24,43]. In the present study, FG in Dioscorea cell culture was mainly represented by protodioscin with only a trace amount of deltoside, which may be specific to cell cultures of this species.…”

“…Accumulation of diosgenin in cell suspension cultures of Dioscorea zingiberensis reached its maximum of 5.25 mg/L after the elicitation treatment using fungal oligosaccharide [46]. Lower FG content determined in the present study for bioreactor-cultured cells compared to shake flask culture was, most likely, associated with mechanical damage and reduced oxygen concentration in a large volume of nutrient medium, which affected cells' respiratory metabolism [24].…”

“…Earlier we reported the development of several strains of suspension cell culture of D. deltoidea as an alternative source of FG protodioscin and deltoside (protodeltonin) specific for this species [23]. The content of protodioscin and deltoside in the cell cultures varied in the range of 3-12% of the dry cell weight depending on culture conditions, which is higher than the average FG content in plants [23][24][25]. One of the strains, DM-05-03, was shown to contain mainly protodioscin and only trace amounts of deltoside which suggests a more specific biological activity [23].…”

Antihypoxic Action of Panax Japonicus, Tribulus Terrestris and Dioscorea Deltoidea Cell Cultures: In Silico and Animal Studies

“… Panax japonicas var. repens – Ginsenosides: Rg1, Malonyl‐Rg1, Rb1, Malonyl‐Rb1, Rb2, Malonyl‐Rb2, Rc, Malonyl‐Rc, Re, Rd, Malonyl‐Rd, Rf, R0, Rh1, Rg2, Notoginsenoside R1, Chikusetsusaponin IVa, Zingibroside R1 [17,18] Dioscorea deltoidea – 25( S )‐Deltoside (25( S )‐protodeltonin); Deltoside (protodeltonin); 25( S )‐Protodioscin (protoneodioscin); Protodioscin [19,20] Tribulus terrestris – Pentahexosyl ‐ hydroxy‐diosgenin (furostanol type); Pentandroside F; 25( R )‐pentandroside F; Hexosyl‐25( S )‐terrestrosin H; Hexosyl‐25( R )‐terrestrosin H; 25( S )‐prototerrestrosin B; 25( R )‐prototerrestrosin B; Terrestrinin B; 25( R )‐terrestrinin B [21] …”

“…Dioscorea deltoidea – 25( S )‐Deltoside (25( S )‐protodeltonin); Deltoside (protodeltonin); 25( S )‐Protodioscin (protoneodioscin); Protodioscin [19,20] …”