A field experiment was conducted during kharif season 2021 to study the effect of seed size and bio-fertilizers on growth, yield and yield parameter of crop. The treatments include different categories of seed size viz., mixed (S1), bold (S2) and small seed size (S3) and bio-fertilizer viz., control (B1), Rhizobium + PSB + KMB (B2) and Trichoderma harzianum + Aspergillus niger (B3). Large seed size and bio-fertilizer shows significant effect on growth parameter viz., plant height, number of branches plant-1, number of pegs plant-1 and root nodules plant-1 and yield parameter viz., number of pods plant-1, number of seeds per pod-1, seed index, shelling percentage and seed yield of groundnut except harvest index. Large seed size shows significant increase in number of pegs plant-1 and pods plant-1 which increase the yield of groundnut. In case of bio-fertilizer, Rhizobium + PSB + KMB shows significant effect on growth and yield parameter viz., plant height, number of branches plant-1, number of pegs plant-1, root nodules plant-1, yield parameter viz., number of pods plant-1, number of seeds per pod-1, seed index, shelling percentage and seed yield of groundnut except harvest index.